本文關鍵詞： 血管內皮生長因子 血漿濃度 多態(tài)性 強直性脊柱炎　出處：《南京醫(yī)科大學》2017年博士論文　論文類型：學位論文

【摘要】：目的:強直性脊柱炎是一種自身免疫性炎癥性疾病,以腰痛,骶髂關節(jié)炎,和脊柱強直為臨床特點。強直性脊柱炎的發(fā)病機制尚不清楚,與血管生成和遺傳背景相關的各種生物標志物在強直性脊柱炎在發(fā)病過程中起重要作用。血管內皮生長因子是一種促進血管內皮細胞遷移和增殖的血管生成因子,具有增加血管通透性和介導炎癥的作用。血管生成在多種風濕性疾病,包括類風濕關節(jié)炎,銀屑病性關節(jié)炎,強直性脊柱炎,系統(tǒng)性硬化癥中發(fā)揮作用,表明抑制血管生成可能是一個有用的治療方法。血管內皮生長因子是最有力的促血管生成分子,并導致強直性脊柱炎的血管生成。我們假設,血管內皮生長因子多態(tài)性和血漿水平可能有助于強直性脊柱炎的易感性。方法:對強直性脊柱炎患者208例和412例住院其他患者對照組的病例對照研究,檢驗強直性脊柱炎風險性,VEGF基因型,中國人群中血管內皮生長因子的血漿水平之間的關聯(lián)。使用基質輔助激光解吸電離飛行時間質譜進行基因分型。對其中120例患者和120名對照組患者,使用酶聯(lián)免疫吸附測定法測定血漿VEGF濃度水平。結果:我們發(fā)現(xiàn),血管內皮生長因子rs3025039 C/T基因多態(tài)性增加強直性脊柱炎的患病風險,在強直性脊柱炎患者組中,血管內皮生長因子的平均血漿濃度相比對照組顯著較高。在強直性脊柱炎患者組中,男性血管內皮生長因子水平明顯高于女性。分層后,rs3025039 CC基因型強直性脊柱炎患者的平均血漿濃度明顯高于對照組。血漿VEGF水平與年齡、HLA-B27、CRP或骶髂關節(jié)評分,在患者組和對照組之間沒有顯著差異。結論:這項研究證據(jù)表明,有可能血管內皮生長因子rs3025039多態(tài)性,血管內皮生長因子血漿濃度和強直性脊柱炎患病風險之間存在聯(lián)系。然而,我們的結果是從一個中等大小的樣品,因此這是一個初步的結論。需在一個更大和更多樣化的種族樣本中驗證,以便確認這些發(fā)現(xiàn)。
[Abstract]:Objective: ankylosing spondylitis is an autoimmune inflammatory disease characterized by lumbago sacroiliac arthritis and spinal ankylosis. Various biomarkers associated with angiogenesis and genetic background play an important role in the pathogenesis of ankylosing spondylitis. Vascular endothelial growth factor (VEGF) is an angiogenic factor that promotes the migration and proliferation of vascular endothelial cells. Angiogenesis plays a role in many rheumatoid diseases, including rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, systemic sclerosis. This suggests that inhibition of angiogenesis may be a useful treatment. Vascular endothelial growth factor is the most potent angiogenic molecule and causes angiogenesis in ankylosing spondylitis. Vascular endothelial growth factor polymorphism and plasma levels may contribute to the susceptibility of ankylosing spondylitis. Methods: a case-control study was conducted in 208 patients with ankylosing spondylitis and 412 other hospitalized controls. To test the genotype of VEGF in the risk of ankylosing spondylitis, Association between plasma levels of vascular endothelial growth factor in Chinese population. Genotyping using matrix assisted laser desorption ionization time of flight mass spectrometry. 120 patients and 120 controls. Results: we found that the polymorphism of vascular endothelial growth factor (rs3025039) C / T gene increased the risk of ankylosing spondylitis in patients with ankylosing spondylitis. The average plasma concentration of vascular endothelial growth factor was significantly higher than that of the control group. After stratification, the mean plasma concentration of VEGF in male was significantly higher than that in female. The average plasma concentration in patients with ankylosing spondylitis genotype rs3025039CC was significantly higher than that in control group. The plasma VEGF level and age of HLA-B27C-CRP or sacroiliac joint score were significantly higher than those in control group. There was no significant difference between the patient group and the control group. Conclusion: the evidence in this study suggests the possibility of vascular endothelial growth factor rs3025039 polymorphism. There is a link between the plasma concentration of vascular endothelial growth factor and the risk of ankylosing spondylitis. So this is a preliminary conclusion. It needs to be tested in a larger and more diverse ethnic sample in order to confirm these findings.
【學位授予單位】：南京醫(yī)科大學
【學位級別】：博士
【學位授予年份】：2017
【分類號】：R593.23

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本文編號：1500530