本文關(guān)鍵詞： 型糖尿病 下肢動(dòng)脈硬化閉塞癥 蛋白組學(xué) 同位素標(biāo)記 二維液相色譜串聯(lián)質(zhì)譜 差異性蛋白　出處：《山東醫(yī)藥》2017年30期 　論文類型：期刊論文

【摘要】：目的通過(guò)蛋白組學(xué)研究,鑒定合并2型糖尿病的下肢動(dòng)脈硬化閉塞癥(DLASO)患者與不合并2型糖尿病下肢動(dòng)脈硬化閉塞癥(LASO)患者血清的差異性蛋白,為防治2型糖尿病患者發(fā)生LASO提供依據(jù)。方法收集DLASO、LASO各15例患者的血清,對(duì)蛋白進(jìn)行提取、定量和酶解,應(yīng)用同位素標(biāo)記相對(duì)和絕對(duì)定量(iTRAQ)結(jié)合二維液相色譜串聯(lián)質(zhì)譜(2DLC-MS/MS)技術(shù),鑒定DLASO、LASO患者血清中的蛋白,篩選出DLASO患者與LASO患者的差異表達(dá)蛋白。利用PANTHER數(shù)據(jù)庫(kù)對(duì)鑒定得到的差異性蛋白進(jìn)行生物學(xué)過(guò)程、分子作用和細(xì)胞組分分析。結(jié)果共鑒定出蛋白質(zhì)338種,篩選出差異性蛋白27種,其中DLASO患者出現(xiàn)表達(dá)上調(diào)蛋白8種、表達(dá)下調(diào)蛋白19種。在生物學(xué)過(guò)程方面,差異性蛋白主要為參與細(xì)胞反應(yīng)(48.1%)、代謝過(guò)程(48.1%)和生物黏附(29.6%)的蛋白;在分子作用方面,差異性蛋白主要為參與細(xì)胞結(jié)合作用(33.3%)、催化活性作用(29.6%)和結(jié)構(gòu)分子活性作用(18.5%)的蛋白;在細(xì)胞組分方面,差異性蛋白主要為位于細(xì)胞區(qū)域(14.8%)、細(xì)胞器(14.8%)和細(xì)胞外區(qū)域(14.8%)的蛋白。結(jié)論發(fā)現(xiàn)DLASO與LASO患者血清中的差異性蛋白27種,這些差異性蛋白可作為防治DLASO的潛在生物學(xué)靶點(diǎn)。
[Abstract]:Objective to study on proteomics. To identify the differential proteins in the serum of patients with type 2 diabetes mellitus (DLASO) and patients without type 2 diabetes mellitus (LASO) of lower extremity arteriosclerosis obliterans (LASO). Methods Serum samples of 15 patients with type 2 diabetes mellitus and 15 patients with DLASOLASO were collected to extract, quantify and hydrolyze the protein. The serum proteins of DLASOLASO patients were identified by isotope labeling and absolute quantitative iTRAQ combined with 2D liquid chromatography tandem mass spectrometry (2DLC-MS / MS) technique. The differentially expressed proteins were screened between DLASO patients and LASO patients. The differential proteins were identified by PANTHER database. Results 338 kinds of proteins were identified and 27 kinds of differential proteins were screened, among which 8 kinds of up-regulated proteins were found in patients with DLASO. There are 19 down-regulated proteins. In the biological process, the differential proteins are mainly involved in the cellular reaction (48.1g), the metabolic process (48.1) and the biological adhesion (29.610). In the aspect of molecular action, the differential proteins were mainly involved in cell binding, catalytic activity, catalytic activity and structural molecular activity, respectively. In terms of cell components, the difference protein is mainly located in the cell area of 14.8%. Conclusion there are 27 different proteins in the serum of patients with DLASO and LASO. These differential proteins can be used as potential biological targets for the prevention and treatment of DLASO.
【作者單位】： 南通大學(xué)附屬醫(yī)院;復(fù)旦大學(xué)附屬中山醫(yī)院;
【基金】：上海市科學(xué)技術(shù)發(fā)展基金資助項(xiàng)目(11ZR1406900)
【分類號(hào)】：R543.5;R587.1
【正文快照】： 全球范圍內(nèi)近2億人口患有2型糖尿病,并且其患病率正在快速增加[1]。下肢動(dòng)脈硬化閉塞癥(LASO)是因動(dòng)脈粥樣硬化累及下肢動(dòng)脈,導(dǎo)致動(dòng)脈狹窄或閉塞而引起的肢體缺血慢性疾病,可以顯著增加心肌梗死、中風(fēng)等嚴(yán)重疾病的發(fā)生率和病死率。合并2型糖尿病時(shí)能加速動(dòng)脈粥樣硬化的進(jìn)展,并，

本文編號(hào)：1486792