發(fā)布時間：2018-02-03 07:25

  本文關鍵詞： 型糖尿病 下肢動脈硬化閉塞癥 蛋白組學 同位素標記 二維液相色譜串聯(lián)質譜 差異性蛋白　出處：《山東醫(yī)藥》2017年30期 　論文類型：期刊論文

【摘要】：目的通過蛋白組學研究,鑒定合并2型糖尿病的下肢動脈硬化閉塞癥(DLASO)患者與不合并2型糖尿病下肢動脈硬化閉塞癥(LASO)患者血清的差異性蛋白,為防治2型糖尿病患者發(fā)生LASO提供依據(jù)。方法收集DLASO、LASO各15例患者的血清,對蛋白進行提取、定量和酶解,應用同位素標記相對和絕對定量(iTRAQ)結合二維液相色譜串聯(lián)質譜(2DLC-MS/MS)技術,鑒定DLASO、LASO患者血清中的蛋白,篩選出DLASO患者與LASO患者的差異表達蛋白。利用PANTHER數(shù)據(jù)庫對鑒定得到的差異性蛋白進行生物學過程、分子作用和細胞組分分析。結果共鑒定出蛋白質338種,篩選出差異性蛋白27種,其中DLASO患者出現(xiàn)表達上調蛋白8種、表達下調蛋白19種。在生物學過程方面,差異性蛋白主要為參與細胞反應(48.1%)、代謝過程(48.1%)和生物黏附(29.6%)的蛋白;在分子作用方面,差異性蛋白主要為參與細胞結合作用(33.3%)、催化活性作用(29.6%)和結構分子活性作用(18.5%)的蛋白;在細胞組分方面,差異性蛋白主要為位于細胞區(qū)域(14.8%)、細胞器(14.8%)和細胞外區(qū)域(14.8%)的蛋白。結論發(fā)現(xiàn)DLASO與LASO患者血清中的差異性蛋白27種,這些差異性蛋白可作為防治DLASO的潛在生物學靶點。
[Abstract]:Objective to study on proteomics. To identify the differential proteins in the serum of patients with type 2 diabetes mellitus (DLASO) and patients without type 2 diabetes mellitus (LASO) of lower extremity arteriosclerosis obliterans (LASO). Methods Serum samples of 15 patients with type 2 diabetes mellitus and 15 patients with DLASOLASO were collected to extract, quantify and hydrolyze the protein. The serum proteins of DLASOLASO patients were identified by isotope labeling and absolute quantitative iTRAQ combined with 2D liquid chromatography tandem mass spectrometry (2DLC-MS / MS) technique. The differentially expressed proteins were screened between DLASO patients and LASO patients. The differential proteins were identified by PANTHER database. Results 338 kinds of proteins were identified and 27 kinds of differential proteins were screened, among which 8 kinds of up-regulated proteins were found in patients with DLASO. There are 19 down-regulated proteins. In the biological process, the differential proteins are mainly involved in the cellular reaction (48.1g), the metabolic process (48.1) and the biological adhesion (29.610). In the aspect of molecular action, the differential proteins were mainly involved in cell binding, catalytic activity, catalytic activity and structural molecular activity, respectively. In terms of cell components, the difference protein is mainly located in the cell area of 14.8%. Conclusion there are 27 different proteins in the serum of patients with DLASO and LASO. These differential proteins can be used as potential biological targets for the prevention and treatment of DLASO.
【作者單位】： 南通大學附屬醫(yī)院;復旦大學附屬中山醫(yī)院;
【基金】：上海市科學技術發(fā)展基金資助項目(11ZR1406900)
【分類號】：R543.5;R587.1
【正文快照】： 全球范圍內近2億人口患有2型糖尿病,并且其患病率正在快速增加[1]。下肢動脈硬化閉塞癥(LASO)是因動脈粥樣硬化累及下肢動脈,導致動脈狹窄或閉塞而引起的肢體缺血慢性疾病,可以顯著增加心肌梗死、中風等嚴重疾病的發(fā)生率和病死率。合并2型糖尿病時能加速動脈粥樣硬化的進展,并，

本文編號：1486792