本文關(guān)鍵詞： sLR11基因多態(tài)性 2型糖尿病 頸動脈IMT　出處：《延邊大學》2016年碩士論文　論文類型：學位論文

【摘要】：研究目的：探討延邊地區(qū)朝鮮族與漢族2型糖尿病患者可溶性低密度脂蛋白受體11(Soluble loW- ensity lipoprotein recptor fami— ly member11, sLR11).單核苷酸多態(tài)性與動脈硬化之間的相關(guān)性。研究對象：選擇2012年12月至2015年12月在延邊大學附屬醫(yī)院內(nèi)分泌門診及住院治療2型糖尿病患者350例,根據(jù)所選研究對象及頸動脈IMT值分為頸動脈粥樣硬化組(CAL組,n=163例,2型糖尿病頸動脈IMT=1.0mm伴斑塊組)和無頸動脈動脈粥樣硬化組(NCAL組,n=187例,2型糖尿病頸動脈IMT1.0mm組)。正常對照組(NGT組,n=172例,健康人)。研究方法：通過彩色超聲診斷儀測定頸動脈IMT及斑塊。利用LDR-PCR技術(shù)進行SNP分型及測序。運用Hardy-Weinberg平衡定律,確定研究樣本的群體代表性。多組間計量資料的比較采用one-way ANOVA,其中兩兩比較采用LSD法比較,應(yīng)用Logistic回歸分析篩選頸動脈粥樣硬化的危險因素,以P0.05為有統(tǒng)計學意義。結(jié)果：1.延邊地區(qū)CAL組、NCAL組、NGT組等位基因A、T頻率分別為54.3%,45.7%; 56.7%,43.3%； 58.1%,41.9%。基因型AA.AT.TT頻率分別為27.6%,53.4%,1 9.0%；32.1%,49.2%,18.7%；30.2%,55.8%,14.0%。經(jīng)檢驗CAL組與NGT組、NCAL組與NGT組、CAL組與NCAL組基因型及等位基因頻率分布均無統(tǒng)計學差異,P0.05。并進行顯性、隱性模式分析,但未見各基因型頻率在三組間比較存在顯著差異,P0.05。2.在CAL組朝鮮族、漢族人群等位基因A、T基因頻率分別為50.7%,49.3％:55.3％,44.7％；AA.AT.TT基因型頻率分別為24.3%,52.7%,23.0%；27.1%,56.5%,16.5%。NCAL組朝鮮族、漢族人群等位基因A、T基因頻率分別為53.3%,46.7%；58.5%,41.5%AA、AT、TT基因型頻率分別為23.7%,59.2%,17.1%;36.8%,43.4%,19.8%。NGT組朝鮮族、漢族人群等位基因A、T基因頻率分別53.8%,46.2%；60.5%,39.5%；AA、AT、TT基因型頻率分別為26.2%,55.4%,18.5%；33.0%,55.0%,12.0%。經(jīng)檢驗CAL組、NCAL組、NGT組中朝鮮族與漢族人群基因型頻率分布均無統(tǒng)計學差異,(P0.05)。3. SBP, LDL-C, HbAlc的回歸系數(shù)分別為-0.024,-0.585, -0.251,回歸系數(shù)與頸動脈硬化成正相關(guān),由此可知,SBP, LDL-C, HbAlc是頸動脈粥樣硬化的危險因素。4.對2型糖尿病頸動脈硬化組中三組基因型進行組間年齡及各代謝指標進行統(tǒng)計學分析,CHO在AT基因型組中高于AA基因型組高于TT基因型組,AT組與AA組、AT組與TT組有統(tǒng)計學差異,P0.05。TG在AT基因型組中高于TT基因型組,兩組之間有統(tǒng)計學差異,P0.01。LDL-C在TT基因型組中高于AT基因型組高于AA基因型組,兩兩比較均有統(tǒng)計學差異,P0.01。結(jié)論：1.sLR11基因rs3824968多態(tài)性與延邊地區(qū)2型糖尿病無明顯相關(guān)性。2.sLR11基因rs3824968多態(tài)性在延邊地區(qū)朝鮮族與漢族人群中無種族差異。3.sLR11基因rs3824968多態(tài)T因子可能通過影響LDL的降解,從而增加T2DM罹患頸動脈粥樣硬化的風險。4. SBP,LDL-C,HbAlc為頸動脈粥樣硬化的危險性因素。
[Abstract]:Objective: to study the soluble low density lipoprotein receptor (sLDLR) 11 in Korean and Han patients with type 2 diabetes mellitus in Yanbian area. Soluble low-ensity lipoprotein recptor fami- ly member11. SLR11). Association of single nucleotide polymorphisms with atherosclerosis. Subjects:. From December 2012 to December 2015, 350 patients with type 2 diabetes mellitus were treated in the endocrine clinic and inpatient department of Yanbian University affiliated Hospital. According to the selected subjects and carotid IMT value, the patients were divided into carotid atherosclerosis group (n = 163) and carotid atherosclerosis group (n = 163). Type 2 diabetic carotid artery IMT=1.0mm with plaque group (n = 187) and no carotid atherosclerosis group (n = 187). Type 2 diabetes mellitus (IMT1.0mm) group and normal control group (n = 172). Healthy persons). Methods: carotid IMT and plaque were determined by color ultrasound. SNP typing and sequencing were performed by LDR-PCR. Hardy-Weinberg equilibrium law was used. One-way ANOVA was used to compare the multi-group measurement data, and the LSD method was used to compare the two groups. Logistic regression analysis was used to screen the risk factors of carotid atherosclerosis, with P0.05 as the statistical significance. Results: 1. Yanbian area CAL group NCAL group. In NGT group, the frequency of Agna T was 54.3% and 45.7%, respectively. 56.7 and 43.3; The frequency of genotype AA.AT.TT was 27.6 and 53.4%, respectively. 32.1 there are 49.2 and 18.7; 30.2NCAL and NGT groups were examined in CAL and NGT groups. There was no significant difference in genotype and allele frequency distribution between CAL group and NCAL group (P 0.05). The dominant and recessive patterns were analyzed. However, there was no significant difference in genotype frequencies among the three groups (P0.05.2). In CAL group, the allelic alleles of Agna T gene were 50.7% in Korean nationality and Han nationality, respectively. 49.3: 55.3and 44.7; The frequencies of AA.AT.TT genotypes were 24. 3% and 52. 7% and 23. 0%, respectively. The frequencies of allelic allele A T gene were 53.33,46.7 in the Korean nationality and the Han nationality in the 56.5and 16.5. NCAL groups, respectively. The frequencies of ATT TT genotypes were 23.7and 59.2and 17.1respectively. The frequencies of allelic allele A T gene in the NGT group were 53.8% and 46.2%, respectively. 60.5 and 39.5; The frequencies of ATT TT genotypes were 26.2and 55.4%, respectively. There was no significant difference in genotype frequency distribution between Korean and Han population in CAL group. The regression coefficients of SBP, LDL-C and HbAlc were -0.024 ~ 0.585 and -0.251 respectively. The regression coefficient was positively correlated with carotid atherosclerosis, which showed that SBP, LDL-C. HbAlc is the risk factor of carotid atherosclerosis. (4) the age and metabolic indexes of three genotypes in type 2 diabetes mellitus with carotid atherosclerosis were analyzed statistically. CHO was higher in AT genotype group than in AA genotype group than in TT genotype group. There was statistical difference between AT group and AA group and AT group and TT group. P0.05.TG in AT genotype group was higher than TT genotype group, there was statistical difference between two groups. P0.01. LDL-C was higher in TT genotype group than in AT genotype group than in AA genotype group. P0.01. conclusion:. 1. The rs3824968 polymorphism of sLR11 gene is not associated with type 2 diabetes mellitus in Yanbian area. 2.sLR11 gene rs3824968 polymorphism in Korean and Han nationality in Yanbian area. The rs3824968 polymorphism T factor of sLR11 gene may influence the degradation of LDL. Therefore, the risk of carotid atherosclerosis in T2DM was increased. 4. SBP LDL-Con HbAlc was the risk factor of carotid atherosclerosis.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R587.2

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