本文關(guān)鍵詞：受體相互作用蛋白140對吡格列酮改善胰島β細(xì)胞糖脂毒性損傷的影響　出處：《中國糖尿病雜志》2016年12期 　論文類型：期刊論文

  更多相關(guān)文章： 受體相互作用蛋白 吡格列酮 胰島β細(xì)胞 糖脂毒性

【摘要】：目的探討過氧化物酶增殖活化受體-γ(PPAR-γ)激動劑吡格列酮對胰島β細(xì)胞糖脂毒性損傷的保護(hù)及受體相互作用蛋白140(RIP140)在其中的介導(dǎo)機(jī)制。方法將胰島β細(xì)胞株MIN6細(xì)胞分為NC組、高糖高脂組、吡格列酮干預(yù)組。穩(wěn)定過表達(dá)RIP140的MIN6細(xì)胞(O-RIP140-MIN6)和過表達(dá)綠色熒光蛋白(GFP)的MIN6細(xì)胞(GFP-MIN6)。分別予高糖高脂(25 rmmol/L葡萄糖+500μmol/L棕櫚酸)和/或10/μmol/L吡格列酮干預(yù)。利用MTT分別檢測各組細(xì)胞增殖率、流式細(xì)胞儀檢測凋亡率、RT-PCR檢測RIP140 mRNA、Western blot檢測B淋巴細(xì)胞瘤-2(Bcl-2)的表達(dá)、硫代巴比妥酸法檢測丙二醛(MDA)水平及黃嘌呤氧化酶法檢測超氧化合物歧化酶(SOD)含量。結(jié)果 NC組、高糖高脂組及吡格列酮干預(yù)組MTT吸光值分別為:24 h(1.80±0.04)、(0.95±0.04)及(0.97±0.03);48 h(2.70±0.11)、(1.04±0.06)及(1.30±0.03)。NC組與高糖高脂組比較,差異有統(tǒng)計(jì)學(xué)意義(24 h:t=25.94,P0.01,48 h:t=24.00,P0.01)。高糖高脂組與吡格列酮干預(yù)組比較,差異有統(tǒng)計(jì)學(xué)意義(48 h:t=9.37,P0.01)。各組間的24 h凋亡率分別為(2.93±0.66)%、(48.08±3.95)%(vs NC組,t=19.54,P0.01)及(31.38±3.92)%(vs高糖高脂組,t=5.20,P0.01)。Bcl-2相對表達(dá)量分別為(1.14±0.06)、(0.42±0.02)(vs NC組,t=20.52,P0.01)及(0.86±0.04)(vs高糖高脂組,t=17.71,P0.01)。RIP140表達(dá)量分別為(1.13±0.11)、(2.34±0.21)(vs NC組,t=9.69,P0.01)及(1.63±0.13)(vs高糖高脂組(t=5.03,P0.01);高糖高脂組與NC組比較,MDA[(10.13±0.47vs(5.00±0.26)nmol/mg,t=16.57,P0.01]、SOD[(5.15±1.07)協(xié)(12.25±1.25)nmol/mg,t=7.51,P0.01]比較,差異均有統(tǒng)計(jì)學(xué)意義。高糖高脂組與吡格列酮干預(yù)組比較,MDA[(10.13±0.47)vs(7.83±0.36)nmol/mg,t=6.77,P0.01]、SOD[(5.15±1.07)v5(8.74±0.59)nmol/mg,t=5.16,P0.01)差異有統(tǒng)計(jì)學(xué)意義。O-RIP140-MIN6和GFP-MIN6細(xì)胞分別給予高糖高脂及吡格列酮處理后,兩組MTT吸光值:24 h(1.04±0.07)vs(1.40±0.16)(t=5.01,P0.01),48 h(1.16±0.13)vs(1.98±0.14)(t=10.73,P0.01)。凋亡率為(41.95±4.88)%vs(31.26±2.86)%(t=2.97,P0.05)、Bcl-2相對表達(dá)為(0.22±0.04)vs(0.76±0.03)(t=21.54,P0.01),SOD為(7.53±0.71)vs(9.62±0.43)nmol/mg(t=4.36,P0.05),MDA為(10.23±0.28)vs(8.15±0.38)nmol/mg(t=7.63,P0.01)。結(jié)論高糖高脂促進(jìn)胰島β細(xì)胞損傷,吡格列酮通過下調(diào)RIP140表達(dá)來抑制高糖高脂對胰島β細(xì)胞的損傷。
[Abstract]:Objective to investigate the peroxisome proliferator activated receptor gamma (PPAR- gamma) receptor agonist pioglitazone and protection of pancreatic islet beta cell glucolipotoxicity injury interacting protein 140 (RIP140) in which the dielectric guide mechanism. Methods MIN6 cells of islet beta cells were divided into NC group, high-fat group, pioglitazone intervention group is stable. Over expression of RIP140 MIN6 cells (O-RIP140-MIN6) and the expression of green fluorescent protein (GFP) MIN6 cells (GFP-MIN6). Were given high fat and high glucose (25 rmmol/L glucose +500 mol/L palmitic acid) and / or 10/ mol/L. Pioglitazone intervention using MTT were detected in cells proliferation rate, the rate of apoptosis was detected by flow cytometry detection of RIP140 mRNA RT-PCR cells, Western blot detection of B lymphocyte in -2 (Bcl-2) expression were measured by thiobarbituric acid (MDA) and xanthine oxidase method to detect superoxide dismutase compounds (SOD) content. The results of N C緇，

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