【摘要】：目的探討青蒿素聯(lián)合羥基氯喹對IgA腎病大鼠的藥理作用。方法采用聯(lián)合牛血清白蛋白(bovine serum album,BSA)+脂多糖(Lipopolysaccharides,LPS)+四氯化碳(carbon tetrachloride,CCl4)方法建立Ig A腎病大鼠模型,選取尿總蛋白較高大鼠共50只,隨機分成5組,即模型對照組、青蒿素與羥基氯喹1∶1組(16.7±16.7)mg·kg~(-1)·d~(-1)、青蒿素聯(lián)合羥基氯喹1∶3組(8.3±25)mg·kg~(-1)·d~(-1)、血尿膠囊組(0.625 g·kg~(-1)·d~(-1))、地塞米松片組(0.078 mg·kg~(-1)·d~(-1)),每組10只,灌胃給藥90 d。另取10只大鼠,不做任何處理,正常飼養(yǎng)作為正常對照組。給藥90 d后,觀察大鼠的一般情況,檢測尿蛋白、血尿、血液生化、腎組織IgA熒光及電鏡情況。結(jié)果與正常對照組比較,模型對照組尿蛋白、血尿及血清肌酐(GRE)、總蛋白(TP)、甘油三酯(TG)均顯著增高(P0.05,P0.01),而血清尿素(UREA)明顯降低(P0.01),血清IgA水平顯著上升(P0.01),腎組織IgA熒光強度(++)~(+++),電鏡檢測腎小球發(fā)生明顯病變。與模型對照組比較,青蒿素聯(lián)合羥基氯喹1∶3組蛋白尿、血清IgA水平顯著降低(P0.01),腎組織IgA熒光強度(+)顯著降低,腎小球病變明顯改善。結(jié)論運用BSA+LPS+CCl4方法造模15周可建立IgA腎病動物模型。青蒿素聯(lián)合羥基氯喹可改善IgA腎病大鼠腎小球濾過膜的屏障功能,可調(diào)節(jié)Ig A腎病大鼠機體免疫反應,降低血清IgA水平,減少免疫復合物IgA沉積,改善IgA腎病大鼠腎臟病理損傷。
[Abstract]:Objective to investigate the pharmacological effects of artemisinin combined with hydroxychloroquine on IgA nephropathy rats. Methods the rat model of Ig A nephropathy was established by combining bovine serum albumin (bovine serum album,BSA lipopolysaccharide (Lipopolysaccharides,LPS) carbon tetrachloride (carbon tetrachloride,CCl4). A total of 50 rats with high urinary total protein were randomly divided into 5 groups. That is, the model control group, artemisinin and hydroxychloroquine 1:1 group (16.7 鹵16.7) mg kg~ (- 1) d ~ (- 1), artemisinin combined with hydroxychloroquine group (8.3 鹵25) mg kg~ (- 1) d ~ (- 1), In the hematuria capsule group (0.625 g kg~ (- 1) d ~ (- 1), dexamethasone tablets group (0.078 mg kg~ (- 1) d ~ (- 1), 10 rats in each group were given intragastrically for 90 days. Another 10 rats were taken without any treatment and fed normally as the normal control group. After 90 days of administration, the general situation of rats was observed, urine protein, hematuria, blood biochemistry, IgA fluorescence and electron microscope in renal tissue were detected. Results compared with the normal control group, the levels of urinary protein, hematuria and serum creatine (GRE), total protein (TP), (TG) in the model control group were significantly higher than those in the normal control group (P0.05, P01). However, serum urea (UREA) decreased significantly (P01), serum IgA level increased significantly (P01). Renal IgA fluorescence intensity () ~ (), electron microscope detected obvious pathological changes in glomeruli. Compared with the model control group, the serum IgA level in artemisinin combined with hydroxychloroquine 1:3 group was significantly lower than that in the model control group (P 0.01), the fluorescence intensity of IgA in renal tissue was significantly decreased, and the Glomerulopathy was significantly improved in artemisinin combined with hydroxychloroquine 1:3 group. Conclusion the animal model of IgA nephropathy can be established by BSA LPS CCl4 method for 15 weeks. Artemisinin combined with hydroxychloroquine can improve the barrier function of renal filtration membrane in rats with IgA nephropathy, regulate the immune response, decrease the level of serum IgA and decrease the deposition of immune complex IgA in rats with IgA nephropathy. To improve the pathological injury of kidney in rats with IgA nephropathy.
【作者單位】： 廣州中醫(yī)藥大學;廣州中醫(yī)藥大學科技產(chǎn)業(yè)園;廣東新南方青蒿科技有限公司;
【基金】：廣東省科技計劃項目(2013B090800013,2013B090800024,2014B040404066,2014YT02S008)
【分類號】：R692.31
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本文編號：2496803