【摘要】：目的比較分析骨髓間充質(zhì)干細(xì)胞(BMSC)經(jīng)腎動脈途徑單次或多次移植于阿霉素腎病大鼠后對其腎功能的改善作用。方法以阿霉素經(jīng)尾靜脈輸入構(gòu)建大鼠慢性腎病模型32只,隨機分為M0、M1、M2、M3組(4×n,n=8),另選8只為正常對照組(N組)。以2×106個/ml、單次0.5 ml分別移植BMSC細(xì)胞0次(M0組)、1次(M1組)、2次(M2組)、3次(M3組),不進(jìn)行BMSC移植組別每次操作以L-DMEM培養(yǎng)基0.5 ml作為安慰劑對照,移植間隔為1周。移植前及末次移植后1、2周末檢測大鼠血尿素氮、血肌酐、24 h尿蛋白、24 h尿微量蛋白,末次移植1周末制作病理片并于激光聚焦顯微鏡下觀察大鼠腎臟病理和BMSC細(xì)胞在腎臟內(nèi)分布情況。結(jié)果 M0、M1、M2、M3組在各觀察點血尿素氮、血肌酐、24 h尿蛋白總量、24 h尿微量蛋白均明顯高于N組(P0.001),末次移植第1周末M2、M3組24 h尿蛋白總量、24 h尿微量蛋白明顯低于M1組(P0.05),M2、M3組間差異無統(tǒng)計學(xué)意義(P=0.063)。結(jié)論 BMSC經(jīng)腎動脈途徑移植2次治療大鼠阿霉素腎病效果最宜。
[Abstract]:Aim to compare the effects of bone marrow mesenchymal stem cells (BMSC) on renal function after transplantation of bone marrow mesenchymal stem cells (MSCs) through renal artery in rats with adriamycin nephropathy. Methods Thirty-two rats were randomly divided into three groups: M _ 0, M _ 1, M _ 2, M _ 3 (4 脳 n, n = 8) and control group (n = 8). BMSC cells were transplanted 0 times (M _ 0 group), 1 time (M _ 1 group), 2 times (M _ 2 group) and 3 times (M _ 3 group) with 2 脳 10 ~ 6 cells / ml, for 0.5 ml. No BMSC transplantation group was performed with L-DMEM medium 0.5 ml as placebo control, the interval of transplantation was 1 week. Blood urea nitrogen, serum creatinine, 24-hour urine protein and 24-hour urine microprotein were measured before transplantation and 1 and 2 weeks after the last transplantation. At the end of the first week after the last transplantation, pathological slices were made and the pathological changes and distribution of BMSC cells in the kidney of rats were observed under laser focusing microscope. Results the levels of blood urea nitrogen, serum creatinine, 24 h urinary protein and 24 h urine microprotein in M0, M1, M2 and M3 groups were significantly higher than those in N group at the end of the first week of transplantation (P0.001). At the end of the first week after the last transplantation, the total urine protein content in M3 group and M3 group was significantly higher than that in N group (P0.001). 24 h urinary microprotein was significantly lower than that of M1 group (P0.05), while there was no significant difference between M2 and M3 groups (P < 0. 063). Conclusion Transplantation of BMSC through renal artery has the best effect on adriamycin nephropathy in rats.
【作者單位】： 昆明醫(yī)科大學(xué)第二附屬醫(yī)院放射治療科;
【分類號】：R692

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 海泉;陳烽烽;周誠;陳向東;高雪華;趙令卉;孫雯;王小燕;李俊;陳靜嫻;趙峻;;臍帶間充質(zhì)干細(xì)胞臨床前安全性考察[J];四川醫(yī)學(xué);2016年03期

2 祁兵;翁孝剛;王濤;曹毓磷;;不同劑量人臍帶間充質(zhì)干細(xì)胞對1型糖尿病大鼠的影響[J];中國衛(wèi)生標(biāo)準(zhǔn)管理;2014年09期

3 Hua-Lian Hang;Qiang Xia;;Role of BMSCs in liver regeneration and metastasis after hepatectomy[J];World Journal of Gastroenterology;2014年01期

4 白彝華;王家平;王劍松;連希艷;蔣紅櫻;李龍;;不同輸注途徑移植骨髓間充質(zhì)干細(xì)胞對阿霉素腎病大鼠療效初探[J];天津醫(yī)藥;2013年12期

5 仙淑麗;劉英莉;方均燕;張薇;;bMSC對腎小管上皮細(xì)胞的保護(hù)作用及其作用機制[J];中華全科醫(yī)學(xué);2013年11期

6 劉韻璐;廖志航;陳東輝;羅雅琪;王愿;郭文;周黎琴;;兩種造模方法致大鼠實驗性腎病模型的腎功能及病理變化比較[J];中藥藥理與臨床;2011年03期

7 程慶;白志明;;間充質(zhì)干細(xì)胞歸巢的研究進(jìn)展[J];中國現(xiàn)代醫(yī)學(xué)雜志;2011年09期

8 王共先;符彥基;;骨髓間充質(zhì)干細(xì)胞移植修復(fù)腎損傷[J];中國組織工程研究與臨床康復(fù);2008年38期

9 許衛(wèi)國;楊建勇;李鶴平;陳偉;莊文權(quán);;建立大鼠肝動脈插管途徑的方法學(xué)研究[J];介入放射學(xué)雜志;2007年04期

10 孫軍輝;滕皋軍;;干細(xì)胞移植治療終末期腎病的實驗研究[J];介入放射學(xué)雜志;2006年10期

相關(guān)碩士學(xué)位論文 前1條

1 蔡文淵;不同劑量干細(xì)胞移植治療急性心肌梗死的薈萃分析[D];大連醫(yī)科大學(xué);2014年

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 邢藝苑;王家平;陸發(fā)承;簡遠(yuǎn)熙;萬珊杉;韓磊;;骨髓間充質(zhì)干細(xì)胞經(jīng)腎動脈途徑不同移植次數(shù)對大鼠阿霉素腎病的修復(fù)作用比較[J];介入放射學(xué)雜志;2017年07期

2 溫智稀;黃晶晶;王振常;熊明鵬;;中醫(yī)藥干預(yù)間充質(zhì)干細(xì)胞治療肝硬化的進(jìn)展[J];遼寧中醫(yī)雜志;2017年06期

3 陸發(fā)承;王家平;邢藝苑;萬珊杉;韓磊;;腎動脈移植骨髓間充質(zhì)干細(xì)胞治療大鼠慢性腎病結(jié)果分析[J];介入放射學(xué)雜志;2017年04期

4 段星祥;王洋;賀菁菁;彭瑩瑩;余娟;孫懿;;干細(xì)胞治療終末期肝病的進(jìn)展[J];中南大學(xué)學(xué)報(醫(yī)學(xué)版);2017年04期

5 謝樹才;張劍權(quán);蔣錫麗;周帥;;骨髓間充質(zhì)干細(xì)胞誘導(dǎo)分化為肝細(xì)胞的方法及機制研究與進(jìn)展[J];中國組織工程研究;2016年50期

6 鄭欣桐;劉欽;張t熛，

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