【摘要】：目的探討沉默信息調(diào)節(jié)因子2-相關(guān)酶3(SIRT 3)對(duì)腎透明細(xì)胞癌細(xì)胞系786-O中錳型超氧化物歧化酶(MnSOD)的去乙�；饔眉捌鋵�(duì)786-O細(xì)胞增殖、凋亡的影響。方法Western blotting和免疫共沉淀(IP)測(cè)定786-O中MnSOD乙�；乃�;MnSOD酶活性試劑盒、四甲基偶氮唑鹽(MTT)和Hoechst熒光染色分別檢測(cè)腎癌細(xì)胞中MnSOD酶活性、細(xì)胞增殖和凋亡。結(jié)果與空白載體(pcDNA3.0)相比,SIRT 3(轉(zhuǎn)染pcDNA3.0-sirt3)使MnSOD乙�；接�1.29±0.16降低為0.74±0.07(t=7.21,P0.001),其酶活性由(1.47±0.17)U/mg增加至(2.53±0.31)U/mg(t=6.70,P0.001)、細(xì)胞增殖率由(25.28±5.75)%增加至(48.86±7.47)%(t=5.60,P0.001),而細(xì)胞凋亡率由(4.53±0.51)%變?yōu)?4.45±0.59)%,差異無(wú)統(tǒng)計(jì)學(xué)意義(t=0.24,P=0.82)。結(jié)論腎透明細(xì)胞癌786-O中MnSOD的去乙�；鰪�(qiáng)其細(xì)胞增殖能力,MnSOD或其乙�；赡苁悄I透明細(xì)胞癌治療的一個(gè)潛在靶點(diǎn)。
[Abstract]:Objective to investigate the deacetylation of manganese-type superoxide dismutase (MnSOD) in renal clear cell carcinoma cell line 786-O by silencing information regulatory factor 2-associated enzyme 3 (SIRT 3) and its effect on proliferation and apoptosis of 786-O cell line. Methods Western blotting and co-immunoprecipitation (IP) were used to detect the level of MnSOD acetylation in 786-O cells, and MnSOD enzyme activity kit, (MTT) and Hoechst fluorescence staining were used to detect MnSOD activity, cell proliferation and apoptosis, respectively. Results compared with blank vector (pcDNA3.0), SIRT 3 (transfection of pcDNA3.0-sirt3) reduced the acetylation level of MnSOD from 1.29 鹵0.16 to 0.74 鹵0.07 (T7.21mP0.001). The enzyme activity increased from (1.47 鹵0.17) U/mg to (2.53 鹵0.31) U/mg (tchr 6.70) P0.001, and the cell proliferation rate increased from (25.28 鹵5.75)% to (48.86 鹵7.47)% (t 5.60 P0.001). However, the rate of apoptosis changed from (4.53 鹵0.51)% to (4.45 鹵0.59)%, and there was no significant difference between them. Conclusion the deacetylation of MnSOD in renal clear cell carcinoma 786-O enhances its proliferative ability. MnSOD or acetylation may be a potential target for the treatment of renal clear cell carcinoma.
【作者單位】： 山東大學(xué)附屬千佛山醫(yī)院小兒外科;山東大學(xué)齊魯醫(yī)學(xué)部基礎(chǔ)醫(yī)學(xué)院癌癥研究中心;山東大學(xué)附屬省立醫(yī)院泌尿外科;
【基金】：山東省科技重大專項(xiàng)(2015ZDXX0802A02) 山東省醫(yī)藥衛(wèi)生科技發(fā)展計(jì)劃(2016WS0481)
【分類號(hào)】：R737.11

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