發(fā)布時(shí)間：2018-11-28 19:45

【摘要】：目的：探討在前列腺癌(PCa)及前列腺上皮內(nèi)瘤(PIN)中上皮特異性粘附分子(EpCAM)的表達(dá)及其臨床意義；探討高NLR值是否與PCa的惡性度及骨轉(zhuǎn)移相關(guān)。 方法：采用免疫組織化學(xué)染色法觀察EpCAM在63例PCa、46例PIN及58例癌旁良性組織的上皮細(xì)胞及間質(zhì)細(xì)胞中的表達(dá)情況；采集110例PCa患者術(shù)前外周靜脈血,計(jì)算NLR值,分析術(shù)前NLR值與患者民族、年齡、血小板計(jì)數(shù)、PCa病理類型、骨轉(zhuǎn)移、血清PSA值之間的關(guān)系。 結(jié)果：(1) EpCAM在PCa和癌旁PIN的上皮細(xì)胞中陽(yáng)性表達(dá)率高于癌旁良性腺體(P0.01)。(2) EpCAM在PCa的間質(zhì)細(xì)胞中陽(yáng)性表達(dá)率高于在癌旁PIN(P0.01)。(3) EpCAM在PCa高中分化組和低分化組間的表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。(4) EpCAM在有骨轉(zhuǎn)移組和無(wú)骨轉(zhuǎn)移組的間質(zhì)細(xì)胞表達(dá)差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。(5)血清PSA值在高NLR組與低NLR組之間的差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論：EpCAM在PCa的間質(zhì)細(xì)胞中的表達(dá)與浸潤(rùn)及骨轉(zhuǎn)移有關(guān),有望成為PCa骨轉(zhuǎn)移的預(yù)測(cè)指標(biāo),進(jìn)一步研究EpCAM在EMT過(guò)程中的作用,為PCa的靶向基因治療提供理論依據(jù)。
[Abstract]:Objective: to investigate the expression and clinical significance of epithelial-specific adhesion molecule (EpCAM) in prostate cancer (PCa) and prostatic intraepithelial tumor (PIN), and to explore whether high NLR value is related to the malignancy of PCa and bone metastasis. Methods: immunohistochemical staining was used to observe the expression of EpCAM in epithelial cells and interstitial cells of 63 cases of PCa,46 and 58 cases of benign tissues adjacent to carcinoma. The peripheral venous blood was collected from 110 patients with PCa before operation and the NLR value was calculated. The relationship between preoperative NLR value and patient's nationality, age, platelet count, PCa pathological type, bone metastasis and serum PSA value was analyzed. Results: (1) the positive expression rate of EpCAM in the epithelial cells of PCa and adjacent PIN was higher than that in the stromal cells of PCa (P0.01). (2) EpCAM was higher than that in adjacent PIN (P0.01). (3). There was no significant difference in the expression of EpCAM between PCa high differentiation group and low differentiation group (P0.05). (4) the expression of EpCAM in interstitial cells of bone metastasis group and non-bone metastasis group had statistical significance (P0.05). (). 5) the difference of serum PSA between high NLR group and low NLR group was statistically significant (P0.05). Conclusion: the expression of EpCAM in the interstitial cells of PCa is related to invasion and bone metastasis, which is expected to be a predictor of PCa bone metastasis. Further study on the role of EpCAM in the process of EMT provides a theoretical basis for PCa targeted gene therapy.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.25

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