【摘要】：目的：探討在前列腺癌(PCa)及前列腺上皮內(nèi)瘤(PIN)中上皮特異性粘附分子(EpCAM)的表達及其臨床意義；探討高NLR值是否與PCa的惡性度及骨轉(zhuǎn)移相關(guān)。 方法：采用免疫組織化學染色法觀察EpCAM在63例PCa、46例PIN及58例癌旁良性組織的上皮細胞及間質(zhì)細胞中的表達情況；采集110例PCa患者術(shù)前外周靜脈血,計算NLR值,分析術(shù)前NLR值與患者民族、年齡、血小板計數(shù)、PCa病理類型、骨轉(zhuǎn)移、血清PSA值之間的關(guān)系。 結(jié)果：(1) EpCAM在PCa和癌旁PIN的上皮細胞中陽性表達率高于癌旁良性腺體(P0.01)。(2) EpCAM在PCa的間質(zhì)細胞中陽性表達率高于在癌旁PIN(P0.01)。(3) EpCAM在PCa高中分化組和低分化組間的表達差異無統(tǒng)計學意義(P0.05)。(4) EpCAM在有骨轉(zhuǎn)移組和無骨轉(zhuǎn)移組的間質(zhì)細胞表達差異具有統(tǒng)計學意義(P0.05)。(5)血清PSA值在高NLR組與低NLR組之間的差異具有統(tǒng)計學意義(P0.05)。 結(jié)論：EpCAM在PCa的間質(zhì)細胞中的表達與浸潤及骨轉(zhuǎn)移有關(guān),有望成為PCa骨轉(zhuǎn)移的預(yù)測指標,進一步研究EpCAM在EMT過程中的作用,為PCa的靶向基因治療提供理論依據(jù)。
[Abstract]:Objective: to investigate the expression and clinical significance of epithelial-specific adhesion molecule (EpCAM) in prostate cancer (PCa) and prostatic intraepithelial tumor (PIN), and to explore whether high NLR value is related to the malignancy of PCa and bone metastasis. Methods: immunohistochemical staining was used to observe the expression of EpCAM in epithelial cells and interstitial cells of 63 cases of PCa,46 and 58 cases of benign tissues adjacent to carcinoma. The peripheral venous blood was collected from 110 patients with PCa before operation and the NLR value was calculated. The relationship between preoperative NLR value and patient's nationality, age, platelet count, PCa pathological type, bone metastasis and serum PSA value was analyzed. Results: (1) the positive expression rate of EpCAM in the epithelial cells of PCa and adjacent PIN was higher than that in the stromal cells of PCa (P0.01). (2) EpCAM was higher than that in adjacent PIN (P0.01). (3). There was no significant difference in the expression of EpCAM between PCa high differentiation group and low differentiation group (P0.05). (4) the expression of EpCAM in interstitial cells of bone metastasis group and non-bone metastasis group had statistical significance (P0.05). (). 5) the difference of serum PSA between high NLR group and low NLR group was statistically significant (P0.05). Conclusion: the expression of EpCAM in the interstitial cells of PCa is related to invasion and bone metastasis, which is expected to be a predictor of PCa bone metastasis. Further study on the role of EpCAM in the process of EMT provides a theoretical basis for PCa targeted gene therapy.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.25

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