【摘要】：目的:研究大鼠左側(cè)精索靜脈曲張(VC)模型及其高位結(jié)扎術(shù)后睪丸生精細(xì)胞凋亡及白細(xì)胞介素-1(IL-1)和一氧化氮(NO)含量的變化。方法:選用雄性SD大鼠60只,均選擇左側(cè)精索靜脈作為研究對象,建立VC模型。將大鼠隨機(jī)分為3組:假手術(shù)組(SO)15只,VC后高位結(jié)扎組(VCT)組15只和VC模型對照組30只。模型對照組中隨機(jī)選取15只大鼠作為VC1組,余下15只大鼠作為VC2組,分別測定VC1組和SO組、VC2組和VCT組大鼠精液質(zhì)量及睪丸組織中IL-1和NO的含量并加以比較,采用TUNEL檢測睪丸生精細(xì)胞凋亡情況。結(jié)果:所有大鼠均建模成功,VC1組精子濃度[(1.54±1.16)×10~6/ml]和精子活力[(44.23±15.46)%]均顯著低于SO組[2.80±1.62)×10~6/ml、(72.34±12.62)%](P0.05),VCT組精子濃度[1.82±1.34)×10~6/ml]和精子活力[(51.21±12.62)%]較VC2組有顯著提高[(1.04±1.21)×10~6/ml、(39.23±13.21)%](P0.05)。大鼠左側(cè)睪丸NO[(0.172±0.030)ng/ml]、IL-1[(1.468±0.080)mg/ml]含量VC1組明顯高于SO組[(0.134±0.021)ng/ml、(0.782±0.079)mg/ml](P0.05),VC2組左側(cè)睪丸NO[(0.198±0.020)ng/ml]、IL-1[(1.994±0.090)mg/ml]含量明顯高于VCT組[(0.141±0.010)ng/ml、(0.781±0.036)mg/ml](P0.05),而右側(cè)睪丸2組比較差異無顯著性(P0.05),而且NO與IL-1含量之間呈正相關(guān)關(guān)系(r=0.492,P0.01)。VC1組大鼠雙側(cè)睪丸生精細(xì)胞大量凋亡,左、右側(cè)睪丸生精細(xì)胞凋亡指數(shù)差異有統(tǒng)計學(xué)意義(P0.05),SO組左、右側(cè)睪丸生精細(xì)胞凋亡指數(shù)無明顯差異(P0.05),2組間同側(cè)睪丸生精細(xì)胞凋亡指數(shù)差異顯著,均有統(tǒng)計學(xué)意義(P0.01);VCT組大鼠左、右側(cè)睪丸生精細(xì)胞凋亡指數(shù)差異有統(tǒng)計學(xué)意義(P0.05);VC2組左、右側(cè)睪丸生精細(xì)胞凋亡指數(shù)無明顯差異(P0.05);2組間同側(cè)睪丸生精細(xì)胞凋亡指數(shù)差異顯著,均有統(tǒng)計學(xué)意義(P0.01)。VC2組、VCT組組內(nèi)同側(cè)睪丸生精細(xì)胞凋亡指數(shù)無明顯差異(P0.05),但VC2組、VCT組2組間同側(cè)睪丸生精細(xì)胞凋亡指數(shù)差異顯著,均有統(tǒng)計學(xué)意義(P0.01)。結(jié)論:VC致睪丸組織中NO和IL-1含量升高,并加重睪丸生精細(xì)胞凋亡,可能是其致睪丸損傷、影響睪丸生精功能障礙的原因之一。
[Abstract]:Aim: to study the changes of testicular spermatogenic cell apoptosis and the contents of interleukin-1 (IL-1) and nitric oxide (no) (NO) in the left varicocele (VC) model of rats after high ligation. Methods: 60 male SD rats were selected and the left spermatic vein was selected as the study object to establish VC model. The rats were randomly divided into three groups: sham operation group (SO) (n = 15), VC post high ligation group (VCT) (n = 15) and VC model control group (n = 30). In the model control group, 15 rats were randomly selected as VC1 group and the remaining 15 rats as VC2 group. The semen quality and the contents of IL-1 and NO in testis of VC1 group and SO group, VC2 group and VCT group were measured and compared respectively. Apoptosis of testicular spermatogenic cells was detected by TUNEL. Results: the sperm concentration and sperm motility in VC1 group [(1.54 鹵1.16) 脳 10~6/ml] and sperm motility [(44.23 鹵15.46)%] were significantly lower than those in SO group [2.80 鹵1.62) 脳 10 ~ (-6) / ml, respectively. (72.34 鹵12.62)%] (P0.05) sperm concentration [1.82 鹵1.34) 脳 10~6/ml] and sperm motility [(51.21 鹵12.62)%] in), VCT group were significantly higher than those in VC2 group [(1.04 鹵1.21) 脳 10 ~ (-6) / ml]. (39.23 鹵13.21)%] (P0.05). The contents of NO [(0.172 鹵0.030) ng/ml] and IL-1 [(1.468 鹵0.080) mg/ml] in left testis of rats in VC1 group were significantly higher than those in SO group [(0.134 鹵0.021) ng/ml, (0.782 鹵0.079) mg/ml] (P0.05). The contents of NO [(0.198 鹵0.020) ng/ml] and IL-1 [(1.994 鹵0.090) mg/ml] in left testis of VC2 group were significantly higher than those in VCT group [(0.141 鹵0.010) ng/ml, (0.781 鹵0.036) mg/ml] (P0.05). However, there was no significant difference between the two groups in the right testis (P0.05), and there was a positive correlation between the content of NO and IL-1 (r = 0.492, P0.01). The number of spermatogenic cells in the testis of the VC1 group was significantly higher than that in the control group (P < 0.05). The apoptosis index of testicular spermatogenic cells in the right testis was significantly different (P0.05) between the), SO group and the right side group (P0.05), but there was significant difference between the two groups in the ipsilateral testicular spermatogenic cell apoptosis index (P0.05). All had statistical significance (P0.01). The apoptosis index of spermatogenic cells in the left and right testis of VCT group was significantly different (P0.05), while that of the left and right testicular spermatogenic cells in VC2 group had no significant difference (P0.05). The apoptotic index of testicular spermatogenic cells in VC2 group and VCT group had no significant difference (P0.05), but in VC2 group, there was no significant difference between the two groups (P0.01), but there was no significant difference between the two groups in the apoptosis index of testicular spermatogenic cells (P0.01). The apoptosis index of testicular spermatogenic cells in VCT group was significantly different (P0.01). Conclusion: VC can increase the contents of NO and IL-1 in testis and aggravate the apoptosis of testicular spermatogenic cells, which may be one of the causes of testicular injury and the dysfunction of testicular spermatogenesis.
【作者單位】： 南京醫(yī)科大學(xué)鼓樓臨床醫(yī)學(xué)院男科;南京軍區(qū)南京總醫(yī)院泌尿外科;
【基金】：國家自然科學(xué)基金資助(81270694) 南京軍區(qū)南京總醫(yī)院立項課題(2015011)~~
【分類號】：R697.24

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