發(fā)布時(shí)間：2018-11-11 08:40

【摘要】：目的研究含碘造影劑是否能夠?qū)е履I小管上皮細(xì)胞壞死。方法體外培養(yǎng)人腎小管上皮細(xì)胞,分為對照組、碘海醇組(75 mg I·m L~(-1))、泛影葡胺組(75 mg I·m L~(-1))。以碘海醇和泛影葡胺干預(yù)細(xì)胞2 h以及藥物移除5,22 h后,用噻唑藍(lán)(MTT)法測定不同時(shí)間點(diǎn)細(xì)胞活力,以乳酸脫氫酶試劑盒測定細(xì)胞上清液乳酸脫氫酶(LDH)釋放;用高內(nèi)涵成像系統(tǒng)對Hoechst33342/PI雙染結(jié)果進(jìn)行定量分析,計(jì)算細(xì)胞凋亡率和死亡率。結(jié)果與對照組相比,碘海醇和泛影葡胺干預(yù)2 h后導(dǎo)致細(xì)胞活力降低,分別為(52.98±6.75)%,(47.16±4.18)%,差異有統(tǒng)計(jì)學(xué)意義(P0.01);同時(shí),還導(dǎo)致LDH釋放增加分別為(201.31±11.69),(256.21±19.82)U·L~(-1),差異有統(tǒng)計(jì)學(xué)意義(P0.01),且藥物移除后5,22 h時(shí)這種影響仍存在。碘海醇組和泛影葡胺組的細(xì)胞凋亡率分別為(23.09±4.25)%,(38.64±8.32)%,細(xì)胞壞死率分別為(19.69±2.13)%,(24.07±6.18)%,與對照組相比,差異有統(tǒng)計(jì)學(xué)意義(P0.01);泛影葡胺導(dǎo)致細(xì)胞壞死更顯著,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論含碘造影劑能夠誘導(dǎo)人腎小管上皮細(xì)胞壞死。
[Abstract]:Objective to study whether iodized contrast agent can cause renal tubular epithelial cell necrosis. Methods Human renal tubular epithelial cells were cultured in vitro and divided into control group and 75 mg I m L ~ (-1), meglumine group (75 mg I m L ~ (-1). The cell viability was determined by thiazolyl (MTT) assay and lactate dehydrogenase (LDH) release from supernatant of cells was determined by lactate dehydrogenase kit after 2 h treatment with iodohydrin and meglumine diatrizoate and 522 h after drug removal. The Hoechst33342/PI double staining results were quantitatively analyzed by high content imaging system, and the apoptosis rate and death rate were calculated. Results compared with the control group, the cell viability was decreased after 2 hours of treatment with iodohexol and meglumine diatrizoate (52.98 鹵6.75)% and (47.16 鹵4.18)%, respectively (P0.01). At the same time, the LDH release was increased by (201.31 鹵11.69), (鹵19.82) U L ~ (-1), the difference was statistically significant (P0.01), and the effect was still present at 5h after drug removal. The apoptotic rate and necrosis rate were (23.09 鹵4.25)%, (38.64 鹵8.32)%, (19.69 鹵2.13)% and (24.07 鹵6.18)%, respectively, in iodohexol group and meglumine diatrizoate group. The difference was statistically significant (P0.01). Diatrizoate meglumine caused more significant cell necrosis, the difference was statistically significant (P0.05). Conclusion Iodine-containing contrast agent can induce the necrosis of human renal tubular epithelial cells.
【作者單位】： 中南大學(xué)湘雅三醫(yī)院藥學(xué)部;
【基金】：教育部新世紀(jì)優(yōu)秀人才支持計(jì)劃基金資助項(xiàng)目(NCET-13-0605) 中南大學(xué)研究生自主探索創(chuàng)新基金資助項(xiàng)目(2016zzts569)
【分類號】：R692

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