【摘要】：目的:探討PDGF-DD/PDGFR-β信號(hào)通路激活對(duì)大鼠腎成纖維細(xì)胞增殖、轉(zhuǎn)化、細(xì)胞外基質(zhì)表達(dá)的影響。方法:體外培養(yǎng)正常大鼠腎成纖維細(xì)胞(NRK-49F),按照PDGF-DD刺激濃度分為對(duì)照組、1ng/ml組、10ng/ml組、50ng/ml組、100ng/ml組,然后根據(jù)50ng/ml PDGF-DD刺激不同時(shí)間分為對(duì)照組、12h組、24h組、48h組,CCK-8方法檢測(cè)不同PDGF-DD濃度及PDGF-DD作用不同時(shí)間對(duì)NRK-49F增殖的影響;Real-time PCR及Western blot檢測(cè)各組PDGFR-β、α-SMA、collagen typeⅠ、FN、MMP-2、TIMP-1 m RNA、蛋白表達(dá)變化。結(jié)果:與對(duì)照組相比,PDGF-DD可明顯刺激NRK-49F增殖,呈現(xiàn)劑量依賴(lài)性(各濃度組細(xì)胞增殖率分別為8.17%,19.17%,31.37%,30.77%,P0.05)及時(shí)間依賴(lài)性(各時(shí)間組細(xì)胞增殖率分別為12.70%,25.07%,39.79%,P0.05)。另外,PDGF-DD可劑量依賴(lài)性刺激PDGF-βR、α-SMA、collagen typeⅠ、FN、TIMP-1在m RNA水平及蛋白水平上的表達(dá)增加,下調(diào)MMP-2的m RNA及蛋白表達(dá)。結(jié)論:PDGF-DD/PDGFR-β信號(hào)通路的激活可顯著刺激NRK-49F的增殖、轉(zhuǎn)化及細(xì)胞外基質(zhì)的沉積,從而導(dǎo)致腎纖維化。
[Abstract]:Aim: to investigate the effects of activation of PDGF-DD/PDGFR- 尾 signaling pathway on proliferation, transformation and extracellular matrix expression of rat renal fibroblasts. Methods: normal rat renal fibroblasts (NRK-49F) were cultured in vitro. According to the concentration of PDGF-DD stimulation, they were divided into control group (1 ng / ml) and 10ng / ml group (50ng / ml). Then CCK-8 was used to detect the changes of PDGFR- 尾, 偽 -SMA-collagen type 鈪，

本文編號(hào)：2202043