本文選題：IgAN + SphK1�。� 參考：《川北醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:檢測(cè)鞘氨醇激酶-1(SphK1)、1-磷酸鞘氨醇受體(S1PR)及α-平滑肌肌動(dòng)蛋白(α-SMA)在IgA腎病(IgAN)患者腎組織中的表達(dá),探討SphK1信號(hào)通路在IgAN發(fā)生發(fā)展中的作用,為IgAN臨床治療提供實(shí)驗(yàn)依據(jù)。方法:選取18例腎穿刺活檢確診為IgAN患者腎組織為實(shí)驗(yàn)組;5例腎腫瘤患者手術(shù)切除遠(yuǎn)離病變部位的正常組織為對(duì)照組。收集兩組患者的臨床資料,如24小時(shí)尿蛋白、血肌酐、尿素氮等。采用免疫組織化學(xué)方法檢測(cè)腎組織中SphK1、S1PR1、S1PR2及α-SMA的表達(dá)。采用Image-ProPlus圖像分析軟件分析光密度值代替相應(yīng)指標(biāo)表達(dá)量。相關(guān)分析法分析SphK1與α-SMA和臨床資料的相關(guān)性。所有數(shù)據(jù)采用spass18.0軟件進(jìn)行統(tǒng)計(jì)分析,計(jì)量資料以均數(shù)±標(biāo)準(zhǔn)差((?)±s)表示,實(shí)驗(yàn)組和對(duì)照組比較采用獨(dú)立樣本t檢驗(yàn),相關(guān)性分析使用Pearson相關(guān)檢驗(yàn),p0.05提示差異有統(tǒng)計(jì)學(xué)意義。結(jié)果:1.在IgAN患者腎組織中SphK1、S1PR2及α-SMA表達(dá)增加,S1PR1/S1PR2的比值降低,與對(duì)照組比較,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);S1PR1表達(dá)水平與對(duì)照組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。2.SphK1與α-SMA表達(dá)水平、24小時(shí)尿蛋白及血肌酐呈正相關(guān)(p0.01或p0.05);SphK1與尿素氮無(wú)直線(xiàn)相關(guān)關(guān)系(P0.05)。結(jié)論:1.SphK1信號(hào)通路可能參與了IgAN發(fā)病機(jī)制;2.SphK1信號(hào)通路在IgAN中的作用可能與α-SMA引起系膜細(xì)胞表型改變有關(guān);3.1-磷酸鞘氨醇(S1P)與其受體亞型結(jié)合過(guò)程中可能存在S1PR1/S1PR2差異性轉(zhuǎn)導(dǎo);4.腎組織中SphK1表達(dá)水平在一定程度上可反映腎臟損害程度;
[Abstract]:Objective: to investigate the expression of sphingosine kinase 1 (SphK1) -1-sphingosine receptor (S1PR) and 偽 -smooth muscle actin (偽 -SMA) in renal tissue of patients with IgA nephropathy (IgAN), and to explore the role of SphK1 signal pathway in the pathogenesis and development of IgAN, and to provide experimental evidence for the clinical treatment of IgAN. Methods: 18 patients with IgAN confirmed by renal biopsy were selected as control group. The clinical data of the two groups were collected, such as 24 hours urine protein, serum creatinine, urea nitrogen, etc. Immunohistochemical method was used to detect the expression of SphK1, S1PR1, S1PR2 and 偽 -SMA in renal tissues. Image-ProPlus image analysis software was used to analyze the optical density instead of the corresponding index expression. The correlation between SphK1 and 偽 -SMA and clinical data was analyzed by correlation analysis. All the data were statistically analyzed by spass18.0 software. The measured data were expressed as mean 鹵standard deviation (?) 鹵s). The experimental group and the control group were compared with the control group by using independent sample t test. The correlation analysis using Pearson correlation test suggested that the difference was statistically significant. The result is 1: 1. The expression of SphK1P1PR2 and 偽 -SMA in renal tissue of IgAN patients increased and the ratio of S1PR1 / S1PR2 decreased. The difference was statistically significant compared with the control group (P0.05) and the expression level of S1PR1 was higher than that of the control group. There was no significant difference between SphK1 and 偽 -SMA expression (P0.05). There was no linear correlation between SphK1 and urea nitrogen (P0.05). Conclusion: 1. SphK1 signaling pathway may be involved in the pathogenesis of IgAN. Secondly, the role of SphK1 signaling pathway in IgAN may be related to the phenotypic changes of Mesangial cells induced by 偽 -SMA. There may be S1PR1 / S1PR2 differential transduction during the binding of sphingosine (S1P) to its receptor subtype. The expression level of SphK1 in renal tissue can reflect the degree of renal damage to some extent.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R692.31

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