發(fā)布時間：2018-06-20 09:02

  本文選題：鹽霉素 + 腫瘤干細胞��； 參考：《實用醫(yī)學(xué)雜志》2017年13期

【摘要】：目的探討鹽霉素對前列腺癌DU145干細胞的影響及其可能的機制,為鹽霉素的臨床應(yīng)用提供理論依據(jù)。方法鹽霉素處理DU145細胞,以ALDH為腫瘤干細胞標記物,用流式細胞儀檢測處理后ALDH陽性的DU145干細胞的百分比。鹽霉素處理DU145細胞后,用Western Blot法檢測mTOR信號通路相關(guān)蛋白m-TOR、p-70s6k、p-p70s6、p-s6等的表達變化。鹽霉素結(jié)合mTOR信號通路抑制劑雷帕霉素處理DU145細胞,利用流式細儀檢測ALDH陽性的DU145干細胞百分比。結(jié)果鹽霉素顯著性抑制ALDH陽性的DU145干細胞(抑制率為77.78%),是紫杉醇的2倍(抑制率為38.64%)。鹽霉素抑制mTOR信號通路相關(guān)分子m-TOR、p-70s6k、p-p70s6、p-s6等蛋白的表達,具有時間依賴性和劑量依賴性。鹽霉素結(jié)合雷帕霉素能夠抑制ALDH陽性的DU145干細胞比例(抑制率為77.95%)。結(jié)論鹽霉素可能通過抑制mTOR通路信號來發(fā)揮抑制腫瘤干細胞的作用。
[Abstract]:Objective to investigate the effect of salinomycin on prostate cancer DU145 stem cells and its possible mechanism, and to provide theoretical basis for the clinical application of salinomycin. Methods DU145 cells were treated with salinomycin and ALDH was used as the marker of tumor stem cells. Flow cytometry was used to detect the percentage of ALDH-positive DU145 stem cells. After treated with salinomycin, the expression of mTOR signaling pathway related protein m-TORp-70s6kN, p-p70s6, p-s6 and so on was detected by Western Blot. DU145 cells were treated with salinomycin combined with rapamycin, an inhibitor of mTOR signaling pathway, and the percentage of ALDH positive DU145 stem cells was detected by flow cytometry. Results Salinomycin significantly inhibited ALDH-positive DU145 stem cells (inhibition rate was 77.78%, twice as much as paclitaxel) (inhibition rate was 38.64%). Salinomycin inhibited the expression of mTOR signaling pathway related molecules m-TOR-p-70s6knp-p70s6p-s6 in a time-and dose-dependent manner. Salinomycin combined with rapamycin could inhibit the percentage of ALDH-positive DU145 stem cells (inhibition rate was 77.95%). Conclusion Salinomycin may inhibit tumor stem cells by inhibiting the signal of mTOR pathway.
【作者單位】： 南華大學(xué)附屬第二醫(yī)院;湖南省岳陽市第一人民醫(yī)院泌尿外科;粵北人民醫(yī)院醫(yī)院泌尿外科;南華大學(xué)第二臨床學(xué)院;
【基金】：國家自然科學(xué)基金資助(編號:81241090) 衡陽市科技計劃項目資助(編號:2016KJ33) 南華大學(xué)“蒸湘學(xué)者”基金資助
【分類號】：R737.25

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1 吳濤;鹽霉素對DU145干細胞的抑制作用及其分子機制[D];南華大學(xué);2016年

2 張郭娟;鹽霉素通過調(diào)節(jié)β-catenin和mTOR信號通路抗前列腺癌干細胞的機理[D];南華大學(xué);2014年

3 歐仁杰;鹽霉素對人膀胱癌5637細胞的生長抑制作用[D];吉林大學(xué);2014年

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本文編號：2043736