本文選題：常規(guī) + 放射�。� 參考：《浙江省醫(yī)學(xué)科學(xué)院》2014年碩士論文

【摘要】：研究目的：電離輻射對人類生殖系統(tǒng)的影響一直備受關(guān)注,已有的大量研究結(jié)果已證實(shí)大劑量的電離輻射對生殖系統(tǒng)有確定的損傷效應(yīng),但低劑量電離輻射的影響仍尚未明確。醫(yī)療照射被認(rèn)為是公眾所接受的最大人工電離輻射照射來源,其中以常規(guī)放射檢查最為常見,但尚未發(fā)現(xiàn)有常規(guī)放射檢查對人類生殖健康的影響研究的報(bào)道。另外,冷凍精液標(biāo)本在長期儲存以及運(yùn)輸過程中可能受到的電離輻射影響仍未明確。因此本研究希望通過觀察常規(guī)放射檢查對人類新鮮精液的精子活力、精子存活率、精子運(yùn)動特征及精子DNA完整性等指標(biāo)的影響,初步探討男性受到低劑量X射線照射后的生殖安全性。 研究方法：21份新鮮精液標(biāo)本來源于浙江省人類精子庫的供精志愿者,禁欲3-5d,手淫取精,精液置37℃恒溫箱液化后將每份精液分成3組(對照組、1mGy組、50mGy組),1mGy組、50mGy組的精液樣本分別接受1mGy(相當(dāng)于普通平片的劑量)和50mGy(相當(dāng)于CT檢查的劑量)的X線照射。x線照射后分別對3組精液樣本的精子活力、精子存活率、精子運(yùn)動特征及精子DNA損傷等指標(biāo)進(jìn)行檢測,精子活力按《世界衛(wèi)生組織人類精液檢查與處理實(shí)驗(yàn)室手冊》(第5版)的標(biāo)準(zhǔn)操作程序,制備濕片后在相差顯微鏡下進(jìn)行評估；精子存活率使用伊紅-苯胺黑染料拒染法檢測,每份樣本觀察的精子數(shù)至少在200個(gè)以上；精子運(yùn)動特征參數(shù)用計(jì)算機(jī)輔助精子分析(CASA)進(jìn)行分析,每份樣本至少要分析200個(gè)活動精子的運(yùn)動軌跡;精子DNA完整性檢測使用基于吖啶橙染色的精子核完整性染色試劑盒,通過流式細(xì)胞儀進(jìn)行檢測。通過配對t檢驗(yàn)比較不同組別指標(biāo)間的差異。 研究結(jié)果： 1、人類新鮮精液經(jīng)不同低劑量X射線照射后精子活力沒有受影響。50mGy組、1mGy組的精子活力(43.52%±27.61%、46.24%±25.24%)與對照組的精子活力(43.14%±28%)沒有顯著性差異,P0.05;50mGy組、1mGy組沒有顯著性差異,P0.05。 2、人類新鮮精液經(jīng)不同低劑量X射線照射后精子存活率沒有受影響。50mGy組、1mGy組的精子活力(84.95%±8.07%、84.33%±8.64%)與對照組的精子活力(84.61%±7.76%)沒有顯著性差異,P0.05;50mGy組、1mGy組沒有顯著性差異,P0.05。 3、人類新鮮精液經(jīng)不同低劑量X射線照射后精子運(yùn)動特征參數(shù)沒有受影響。50mGy組、1mGy組以及對照組平均的直線速率(VCL)、曲線速率(VSL)、平均路線速率(VAP)、精子頭側(cè)擺幅度(ALH)、直線性(LIN)、前向行(STR)、鞭打頻率(BCF)均沒有統(tǒng)計(jì)學(xué)差異,P0.05。 4、人類新鮮精液經(jīng)不同低劑量X射線照射后精子DNA碎片指數(shù)(DFI)沒有受影響。50mGy組、1mGy組的DFI(15.75%±10.91%、16.29%±12.76%)與對照組的DFI(16.48%±13.37%)沒有顯著性差異,P0.05；50mGy組、1mGy組沒有顯著性差異,P0.05。 5、人類新鮮精液經(jīng)不同低劑量X射線照射后精子高DNA可染性(HDS)沒有受影響。50mGy組、1mGy組的HDS(9.26%±4.23%、9.14%±4.25%)與對照組的HDS(9.29%±3.59%)沒有顯著性差異,P0.05；50mGy組、1mGy組沒有顯著性差異,P0.05。 研究結(jié)論：我們的研究表明50mGy及以下的低劑量X射線對人類新鮮精液的精子活力、精子存活率、精子運(yùn)動特征參數(shù)以及精子DNA完整性等指標(biāo)沒有影響,因此,我們認(rèn)為常規(guī)的放射學(xué)檢查(平片、CT)對男性離體成熟的精子是相對安全的,對評估冷凍精液在長途運(yùn)輸中接受X射線安檢的影響具有參考意義。但有關(guān)低劑量X射線對男性整體生育功能是否有影響,還需進(jìn)一步深入研究。
[Abstract]:The effects of ionizing radiation on the reproductive health of human beings have been paid attention to . The effects of high doses of ionizing radiation on reproductive health have not been clarified .

The sperm motility , sperm viability , sperm motility and sperm DNA damage were measured in 3 groups ( control group , 1 mGy group , 50 mGy group ) , 1 mGy group and 50 mGy group respectively .
Sperm viability was detected using the eosin - aniline black dye exclusion assay , and the number of sperm observed per sample was at least 200 ;
The sperm motility parameters were analyzed by computer - assisted sperm analysis ( CASA ) , at least 200 active sperm motility trajectories were analyzed for each sample , and sperm DNA integrity testing was performed using an acridine orange stain - based sperm nucleus integrity staining kit , which was detected by flow cytometry . Differences between different groups were compared by paired t tests .

Results of the study :

1 . There was no significant difference between the sperm motility ( 43.52 % 鹵 27.61 % , 46.24 % 鹵 25.24 % ) and the sperm motility ( 43.14 % 鹵 28 % ) in the 50 mGy group and 1 mGy group . There was no significant difference in sperm motility ( 43.14 % 鹵 28 % ) in the control group .

There was no significant difference in sperm motility ( 84.95 % 鹵 8.07 % , 84.33 % 鹵 8.64 % ) in 50 mGy group and 1 mGy group ( 84.33 % 鹵 8.64 % ) and control group ( 84.61 % 鹵 7.76 % ) .

3 . The parameters of sperm motility were not influenced by human fresh semen after X - ray irradiation at different doses . The average linear velocity ( VCL ) , curve rate ( VCL ) , average route rate ( vap ) , head - side swing amplitude ( ALH ) , linearity ( LIN ) , forward line ( STR ) , and whips frequency ( Bp ) in 50 mGy group , 1 mGy group and control group were not statistically significant , P0.05 .

4 . There was no significant difference in the sperm DNA fragment index of human fresh semen after low dose X - ray irradiation ( 15.75 % 鹵 10.91 % , 16.29 % 鹵 12.76 % ) in the 50mGy group and 1 mGy group compared with the control group ( 16.48 % 鹵 13.37 % ) , P0.05 ;
There was no significant difference between the 50mGy group and the 1mGy group ( P0.05 ) .

5 . There was no significant difference between the HDS ( 9.26 % 鹵 4.23 % , 9.14 % 鹵 4.25 % ) and HDS ( 9.29 % 鹵 3.59 % ) of the control group ( P0.05 ) .
There was no significant difference between the 50mGy group and the 1mGy group ( P0.05 ) .

Conclusion : Our study shows that 50 mGy and lower dose X - rays have no influence on sperm motility , sperm viability , sperm motility , sperm DNA integrity , and so on . Therefore , we believe that routine radiological examination ( plain film , CT ) is relatively safe for male ex vivo sperm . However , it is of reference to evaluate the effect of low - dose X - ray on the whole reproductive function of men .
【學(xué)位授予單位】：浙江省醫(yī)學(xué)科學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R698.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 徐志榮;徐汀;李晶;楊舒波;李朝偉;;多層螺旋CT電離輻射的探討[J];中國醫(yī)學(xué)裝備;2011年12期

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本文編號：2002530