本文選題：絕經(jīng)期女性 + 泌尿道菌群��； 參考：《青島大學(xué)》2017年碩士論文

【摘要】：目的:通過16S rRNA基因高通量測序的非培養(yǎng)檢測方法,研究伴有尿路感染癥狀的絕經(jīng)期女性和健康絕經(jīng)期女之間泌尿道細菌結(jié)構(gòu)的差異。方法:收集就診于青島大學(xué)附屬醫(yī)院腎病內(nèi)科門診的有尿路感染癥狀的絕經(jīng)期女性14例,留取其尿液,將尿液樣本分為兩份,一份進行細菌基因組DNA提取,應(yīng)用16S rRNA高通量測序的方法檢測尿液樣本中細菌的種類;另外一份送至細菌室進行尿培養(yǎng)檢查。收集同時期于青島大學(xué)附屬醫(yī)院體檢中心健康體檢的絕經(jīng)期女性,共14例,留取其尿液,同樣進行細菌基因組DNA提取及16SrRNA測序。分析兩組女性的泌尿道細菌結(jié)構(gòu)之間差異。結(jié)果:(1)測序結(jié)果顯示兩組女性的尿液菌群構(gòu)成均呈多樣化,但個體差異較明顯。在所有的尿液樣本中,共檢測出385個屬的細菌類型,平均每個尿液樣本包含25個屬的細菌。其中檢測到最豐富的細菌前四位分別是是厚壁菌門、放線菌門、變形菌門和擬桿菌門。在屬的水平上,檢測最多的前十位細菌為乳酸桿菌屬、加德納菌屬、棒狀桿菌屬、腸桿菌科、葡萄球菌屬、鏈球菌屬、厭氧球菌屬、嗜胨菌屬、普氏菌屬、放線菌屬。(2)有尿路感染癥狀的絕經(jīng)期女性尿液中加德納菌屬、氣球菌屬的檢出率顯著高于正常對照組,乳酸桿菌的檢出率顯著低于正常對照組,差異有統(tǒng)計學(xué)意義(P0.05)。其他細菌如腸桿菌科、厭氧球菌屬、放線棒菌屬、放線菌屬、纖毛菌屬、普氏菌屬、解脲寡源桿菌屬等在實驗組中被檢測到的比例較對照組高,在對照組中比例較高的菌屬有鏈球菌屬、產(chǎn)黃桿菌屬、嗜胨菌屬、雙歧桿菌屬等,但因本實驗樣本量少等原因,以上兩組的差異不具有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:絕經(jīng)期女性的尿液菌群構(gòu)成呈多樣化,檢測到最豐富的細菌是厚壁菌門、放線菌門、變形菌門和擬桿菌門。樣本中以乳酸桿菌為優(yōu)勢菌的最多,其次是加德納菌。有尿路感染癥狀的絕經(jīng)期女性患者泌尿道菌群表現(xiàn)為乳酸桿菌顯著減少,而加德納菌屬、氣球菌數(shù)量增加。提示伴隨尿路感染癥狀的絕經(jīng)期女性泌尿道菌群結(jié)構(gòu)發(fā)生了改變,可能是引起絕經(jīng)期婦女尿路感染發(fā)病率升高的重要原因。
[Abstract]:Objective: to study the difference of urinary tract bacterial structure between menopausal women with urinary tract infection and healthy menopausal women by using 16s rRNA gene high-throughput sequencing method. Methods: fourteen postmenopausal women with urinary tract infection symptoms were collected from the Department of Nephrology, affiliated Hospital of Qingdao University. Urine samples were collected and divided into two parts. One sample was extracted from bacterial genome DNA. The bacterial species in urine samples were detected by 16s rRNA high-throughput sequencing, and the other samples were sent to the bacteria room for urine culture. A total of 14 postmenopausal women who were examined in the physical examination Center of affiliated Hospital of Qingdao University in the same period were collected. Urine samples were collected and the genomic DNA of bacteria was extracted and 16SrRNA sequenced. The differences of urinary tract bacterial structure between the two groups were analyzed. Results: the results of sequencing showed that the composition of urine flora of the two groups was diverse, but the individual difference was obvious. A total of 385 genera of bacteria were detected in all urine samples, with an average of 25 genera per urine sample. The four most abundant bacteria detected were phylum thunbergiformis, actinomycetes, Proteus and Bacteroides. At the genus level, the top 10 most detected bacteria were Lactobacillus, Gardnebacterium, Corynebacterium, Enterobacteriaceae, Staphylococcus, Streptococcus, Anaerobes, peptone, Platella, The detection rate of Gardnerella in menopausal women with urinary tract infection was significantly higher than that of normal controls, and the detection rate of Lactobacillus was significantly lower than that of normal controls (P 0.05). Other bacteria, such as Enterobacteriaceae, Anaerobes, Actinomycetes, Actinomyces, Cilium, Platinum, Urea-Oligomonas, and so on, were detected more frequently in the experimental group than in the control group. In the control group, there were streptococcus, xanthobacterium, peptone, bifidobacterium and so on. However, the difference between the two groups was not statistically significant because of the small sample size in this experiment. Conclusion: the composition of urine flora of menopausal women is diverse, and the most abundant bacteria detected are phylum thunbergiformis, actinomycetes, Proteus and Bacteroides. Lactobacillus was the dominant bacteria in the sample, followed by Gardner's. The urinary tract flora of menopausal women with urinary tract infection symptoms was significantly decreased, while the number of balloon bacteria increased in Gardner's genus. It is suggested that the changes of urinary tract flora in menopausal women with urinary tract infection symptoms may be an important reason for the increase of urinary tract infection in menopausal women.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R691.3

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