本文選題：膀胱尿路上皮細胞癌 + 半乳糖凝集素-3　； 參考：《安徽醫(yī)科大學》2014年碩士論文

【摘要】：目的通過檢測半乳糖凝集素-3（Galectin-3）和基質金屬蛋白酶-9（MMP-9）在膀胱尿路上皮細胞癌（BUCC）組織中的表達情況，分析兩者與BUCC臨床病理參數(shù)的關系，，探討Galectin-3和MMP-9在BUCC的發(fā)生、發(fā)展及預后的臨床意義。 方法采用免疫組織化學鏈霉菌抗生物素蛋白-過氧化物酶（S-P）方法，對67例BUCC（其中T1期17例，T2期23例，T3期20例，T4期7例）和12例正常膀胱組織中Galectin-3和MMP-9的表達進行檢測，并計數(shù)BUCC腫瘤組織的微血管密度（MVD）。結合臨床病理數(shù)據(jù)，應用統(tǒng)計學方法分析兩者與BUCC的腫瘤大小、病理分級、臨床分期、淋巴轉移、復發(fā)性和MVD等，以及與BUCC患者的年齡、性別和術后生存率的關系。 結果BUCC組織中，Galectin-3和MMP-9陽性表達率為62.69%和73.13%，分別高于在正常膀胱組織的16.67%和0.00%，差異有統(tǒng)計學意義（P0.05）；BUCC臨床分期及病理分級越高，兩者陽性表達率越高，有淋巴結轉移者較無轉移者陽性表達率高，復發(fā)者較初發(fā)者高，差異有統(tǒng)計學意義（P0.05）；Galectin-3和MMP-9陽性表達率在不同性別、不同年齡和不同腫瘤大小之間無明顯差異，無統(tǒng)計學意義（P0.05）。MMP-9在Galectin-3表達陽性的BUCC中的陽性表達率較Galectin-3陰性中的高；相應的，Galectin-3在MMP-9表達陽性的BUCC組織中的陽性表達率較MMP-9陰性中的高。兩者在BUCC中表達呈正相關，存在統(tǒng)計學意義（χ2=22.284，列聯(lián)系數(shù)r=0.500，P 0.05）。MVD在Galectin-3和MMP-9呈現(xiàn)陽性表達的BUCC組織中高于陰性者，差異有統(tǒng)計學意義（P 0.05）。Galectin-3和MMP-9表達陽性的患者術后5年生存率低于表達陰性者，差異有統(tǒng)計學意義（P0.05）。 結論Galectin-3和MMP-9與BUCC病理學分級、臨床分期、淋巴結轉移及復發(fā)密切相關，可能與BUCC的發(fā)生、進展、侵犯及轉移有關，且兩者可能存在某種協(xié)同效應；兩者對BUCC組織腫瘤血管生成可能具有某種上調作用，對BUCC發(fā)生及發(fā)展機制研究可能有科研價值；兩者與患者術后生存率密切相關，可能成為BUCC手術治療的有效預后指標。
[Abstract]:Objective to investigate the expression of Galectin-3 (galectin-3) and matrix metalloproteinase-9 (MMP-9) in bladder urinary tract epithelial cell carcinoma (BUCC), and to analyze the relationship between them and the clinicopathological parameters of BUCC, and to explore the occurrence of Galectin-3 and MMP-9 in BUCC. Clinical significance of development and prognosis. Methods the expression of Galectin-3 and MMP-9 in 67 cases of bucca (17 cases of T 1, 23 cases of stage T 3, 7 cases of phase 4 of T 3) and 12 cases of normal bladder tissues were detected by immunohistochemical streptomyces biotin-peroxidase S-P method. The microvessel density of BUCC tumor tissue was counted. Combined with clinicopathological data, the relationship between tumor size, pathological grade, clinical stage, lymphatic metastasis, recurrence and MVD, and age, sex and postoperative survival rate of BUCC patients were analyzed by statistical method. Results the positive expression rates of Galectin-3 and MMP-9 in BUCC were 62.69% and 73.13, respectively, which were higher than those in normal bladder tissue (16.67% and 0.005%, respectively). The higher the clinical stage and pathological grade were, the higher the positive expression rate was. The positive expression rate of Galectin-3 and MMP-9 in patients with lymph node metastasis was higher than that in patients with lymph node metastasis, and the positive rates of Galectin-3 and MMP-9 in patients with recurrent lymph nodes were higher than those without metastasis. There was no significant difference in the positive expression rates of Galectin-3 and MMP-9 between different genders, different ages and different tumor sizes. The positive expression rate of MMP-9 in Galectin-3 positive BUCC was higher than that in Galectin-3 negative BUCC, and the positive rate of MMP-9 positive BUCC was higher than MMP-9 negative. There was a positive correlation between them in BUCC and there was a significant difference between them (蠂 ~ 2, P = 22.284, r = 0.500 P 0.05).MVD). The positive expression of P 0.05).MVD in Galectin-3 and MMP-9 was higher than that in the negative ones. The 5-year survival rate of patients with positive expression of P 0.05).Galectin-3 and MMP-9 was significantly lower than that of patients with negative expression of P 0.05).Galectin-3 and MMP-9, and the difference was statistically significant (P 0.05). Conclusion Galectin-3 and MMP-9 are closely related to the pathological grading, clinical stage, lymph node metastasis and recurrence of BUCC, and may be related to the occurrence, progression, invasion and metastasis of BUCC, and there may be some synergistic effect between them. Both of them may have some up-regulation effect on tumor angiogenesis in BUCC tissues, and may be of scientific value in the study of the pathogenesis and development of BUCC, and they are closely related to the survival rate of the patients after operation, and may be an effective prognostic index for the treatment of BUCC.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.14

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