本文選題：P凋亡刺激蛋白 + 內(nèi)質(zhì)網(wǎng)應(yīng)激�。� 參考：《廣東醫(yī)學(xué)》2017年16期

【摘要】：目的探討P53凋亡刺激蛋白(ASPP)家族成員ASPP2蛋白及內(nèi)質(zhì)網(wǎng)應(yīng)激在老年大鼠腎臟損傷中的作用機制。方法以6月齡SD大鼠為成年組,18月齡SD大鼠為老年組,蘇木精-伊紅染色(HE)觀察各組大鼠腎臟結(jié)構(gòu)的改變;qRT-PCR檢測內(nèi)質(zhì)網(wǎng)應(yīng)激相關(guān)指標C/EPB同源蛋白(CHOP)、活化轉(zhuǎn)錄因子6(ATF6)、X盒結(jié)合蛋白-1(XBP-1)、葡萄糖調(diào)節(jié)蛋白78(GRP78)的mRNA表達水平;Western blot法檢測各組大鼠ASPP2蛋白的表達水平,并對ASPP2的表達水平與內(nèi)質(zhì)網(wǎng)應(yīng)激相關(guān)指標CHOP、ATF6、XBP-1、GRP78的表達水平作相關(guān)性分析。結(jié)果與成年組SD大鼠相比,老年組SD大鼠腎臟結(jié)構(gòu)明顯改變,CHOP、ATF6、XBP-1、GRP78的mRNA表達水平及ASPP2蛋白表達水平顯著增高,且ASPP2水平與CHOP和XBP-1呈正相關(guān),ASPP2水平與ATF6呈負相關(guān)。結(jié)論在老年SD大鼠中,ASPP2調(diào)控內(nèi)質(zhì)網(wǎng)應(yīng)激可能是引起腎臟損傷的重要機制之一。
[Abstract]:Objective to investigate the role of ASPP2 protein and endoplasmic reticulum stress (ER) in renal injury in aged rats. Methods Sprague-Dawley rats aged 6 months were used as adult group and 18 months old SD rats as aged group. The changes of renal structure in rats were observed by hematoxylin and eosin staining. The expression level of C/EPB homologous protein (C/EPB homologous protein), the activation transcription factor 6 (ATF6) X box binding protein-1 (XBP-1) and the glucose-regulated protein 78 (GRP78) were detected by mRNA blot method in each group. The expression level of ASPP2 protein was detected in each group. Correlation analysis was made between the expression level of ASPP2 and the expression level of the endoplasmic reticulum stress related index CHOPF6 XBP-1GRP78. Results compared with the adult SD rats, the renal structure of SD rats in the aged group was significantly changed. The expression of mRNA and ASPP2 protein were significantly increased in the rat kidney of CHOPP-ATF6, XBP-1, GRP78, and the level of ASPP2 was positively correlated with CHOP and XBP-1 and negatively correlated with ATF6. Conclusion the regulation of endoplasmic reticulum stress by ASPP2 may be one of the important mechanisms of renal injury in aged SD rats.
【作者單位】： 寧夏醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院;四川大學(xué)華西基礎(chǔ)醫(yī)學(xué)與法醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(編號:81360063,81460121)
【分類號】：R692

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