本文選題：腎臟纖維化 + 腎臟鈣化　； 參考：《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年12期

【摘要】：目的·探討胰島素樣因子6(Insl6)對(duì)單側(cè)輸尿管梗阻(UUO)小鼠腎臟纖維化和鈣化的影響。方法·SPF級(jí)C57BL/6雄性小鼠24只,隨機(jī)分為3組,假手術(shù)組(n=8)、UUO+生理鹽水組(n=8)和UUO+Insl6組(n=8)。UUO模型建立后第10日處死小鼠,取術(shù)側(cè)腎組織。采用天狼星紅染色、Masson染色、茜素紅S染色分別檢測(cè)腎臟膠原沉積及鈣化程度,Western blotting檢測(cè)腎臟TGF-β1表達(dá),realtime-PCR檢測(cè)腎臟TGF-β1、BMP2、Col1a1、Col2a1 m RNA表達(dá)。結(jié)果·與假手術(shù)組相比,UUO+生理鹽水組腎臟膠原面積百分比顯著增加,以Ⅰ型膠原為主,鈣鹽沉積,TGF-β1蛋白表達(dá),TGF-β1、BMP2、Col1a1、Col2a1 m RNA表達(dá)均顯著增加(均P0.05)。與UUO+生理鹽水組相比,UUO+Insl6組小鼠腎臟膠原面積,鈣鹽沉積,TGF-β1蛋白,TGF-β1、BMP2、Col1a1、Col2a1 m RNA表達(dá)均顯著降低(均P0.05)。結(jié)論·Insl6具有抗UUO誘導(dǎo)的腎臟纖維化及鈣化作用,其機(jī)制可能與調(diào)控TGF-β1、collagenⅠ、BMP2、collagenⅡ表達(dá)水平有關(guān)。
[Abstract]:Objective to investigate the effects of insulin-like factor 6 (Insl6) on renal fibrosis and calcification in UUO mice with unilateral ureteral obstruction. Methods Twenty-four C57BL/6 male mice of SPF grade were randomly divided into 3 groups: sham operation group (n = 8) and UUO Insl6 group (n = 10). Sirius red staining and alizarin red S staining were used to detect the collagen deposition in kidney and the expression of TGF- 尾 1 in kidney by blotting. The expression of TGF- 尾 1 in kidney was detected by time-PCR. The expression of TGF- 尾 1 BMP2Col1a1 and Col2a1 m RNA in kidney were detected. Results compared with sham operation group, the percentage of collagen area in kidney of UUO saline group was significantly increased, mainly type 鈪，

本文編號(hào)：1911059