本文選題：腎小管上皮細(xì)胞 + 雷洛昔芬　； 參考：《山東醫(yī)藥》2016年30期

【摘要】：目的觀察應(yīng)用雷洛昔芬對(duì)鎘染毒的腎小管上皮細(xì)胞存活情況及1-α羥化酶表達(dá)的影響,探討雷洛昔芬治療鎘中毒導(dǎo)致腎毒性的機(jī)制。方法分離純化SD大鼠腎小管上皮細(xì)胞原代細(xì)胞。分別用濃度為2.5、5.0、10.0、20.0、40.0μmol/L的氯化鎘對(duì)腎小管上皮細(xì)胞染毒24 h,采用MTT法測(cè)算腎小管上皮細(xì)胞的相對(duì)存活率。選取20.0μmol/L的氯化鎘劑量作用于腎小管上皮細(xì)胞,分別用0.05、0.10、0.50、1.00μmol/L的雷洛昔芬干預(yù)24h,采用MTT法測(cè)算腎小管上皮細(xì)胞的相對(duì)存活率。將腎小管上皮細(xì)胞分為空白對(duì)照組、氯化鎘組、雷洛昔芬組,空白對(duì)照組培養(yǎng)于DMEM培養(yǎng)基,氯化鎘組以20.0μmol/L的氯化鎘染毒腎小管上皮細(xì)胞,雷洛昔芬組以20.0μmol/L的氯化鎘染毒后給予雷洛昔芬0.10μmol/L干預(yù),均干預(yù)24 h,在顯微鏡下觀察細(xì)胞形態(tài),采用RT-PCR法檢測(cè)腎小管上皮細(xì)胞1-α羥化酶mRNA表達(dá)。結(jié)果 2.5、5.0、10.0、20.0、40.0μmol/L的氯化鎘干預(yù)24 h后,腎小管上皮細(xì)胞的相對(duì)存活率均降低(P均0.01),且氯化鎘濃度較高時(shí)細(xì)胞相對(duì)存活率較低。對(duì)鎘染毒后的腎小管上皮細(xì)胞以0.05、0.10、0.50、1.00μmol/L的雷洛昔芬干預(yù)24 h后,腎小管上皮細(xì)胞的相對(duì)存活率均高于鎘染毒細(xì)胞(P均0.01)�？瞻讓�(duì)照組腎小管上皮細(xì)胞形態(tài)正常,氯化鎘組腎小管上皮細(xì)胞形態(tài)異常、大量細(xì)胞死亡,雷洛昔芬組細(xì)胞形態(tài)異常較氯化鎘組明顯減輕。氯化鎘組腎小管上皮細(xì)胞1-α羥化酶mRNA相對(duì)表達(dá)量低于空白對(duì)照組,雷洛昔芬組1-α羥化酶mRNA相對(duì)表達(dá)量高于氯化鎘組(P均0.05)。結(jié)論雷洛昔芬對(duì)氯化鎘染毒的腎小管上皮細(xì)胞具有保護(hù)作用,可上調(diào)鎘染毒后腎小管上皮細(xì)胞1-α羥化酶mRNA的表達(dá)。
[Abstract]:Objective to observe the effects of raloxifene on the survival of cadmium-exposed renal tubular epithelial cells and the expression of 1- 偽 -hydroxylase, and to explore the mechanism of raloxifene in the treatment of renal toxicity induced by cadmium poisoning. Methods SD rat renal tubular epithelial cells were isolated and purified. The relative survival rate of renal tubular epithelial cells was measured by MTT assay after exposure to cadmium chloride of 2.5V 5.0 渭 mol/L (10.0 渭 mol/L) for 24 hours. The renal tubular epithelial cells were treated with 20 渭 mol/L cadmium chloride for 24 h. The relative survival rate of renal tubular epithelial cells was measured by MTT method. Renal tubular epithelial cells were divided into blank control group, cadmium chloride group, raloxifene group, blank control group, cultured in DMEM medium. Cadmium chloride group was exposed to 20 渭 mol/L cadmium chloride on renal tubular epithelial cells. Raloxifene group was treated with 20 渭 mol/L cadmium chloride for 24 h. The cell morphology was observed under microscope and the expression of 1- 偽 hydroxylase mRNA in renal tubular epithelial cells was detected by RT-PCR method. Results the relative survival rate of renal tubule epithelial cells was decreased after 24 hours of cadmium chloride treatment with 2.55.010.0 渭 mol/L (20.0 渭 mol/L), and the relative survival rate of renal tubular epithelial cells was lower when cadmium chloride concentration was higher than that of the control group (P < 0.05), and the relative survival rate of renal tubular epithelial cells was lower when the concentration of cadmium chloride was higher. The relative survival rate of renal tubular epithelial cells exposed to cadmium was higher than that of cadmium exposed cells (P < 0.01). The morphology of renal tubular epithelial cells in the blank control group was normal, and that in the cadmium chloride group was abnormal and a large number of cells died. The abnormal morphology of the renal tubular epithelial cells in the raloxifen group was significantly less than that in the cadmium chloride group. The relative expression of 1- 偽 -hydroxylase mRNA in renal tubular epithelial cells in cadmium chloride group was lower than that in control group, and the relative expression of 1- 偽 -hydroxylase mRNA in raloxifene group was higher than that in cadmium chloride group (P < 0.05). Conclusion Riloxifene has protective effect on renal tubular epithelial cells exposed to cadmium chloride and can up-regulate the expression of 1- 偽 -hydroxylase mRNA in renal tubular epithelial cells after cadmium exposure.
【作者單位】： 桂林醫(yī)學(xué)院附屬醫(yī)院;
【基金】：廣西醫(yī)療衛(wèi)生重點(diǎn)科研課題(重2012011)
【分類號(hào)】：R114;R692

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