本文選題：高糖 + 全反式維甲酸�。� 參考：《中國(guó)老年學(xué)雜志》2017年01期

【摘要】：目的探討全反式維甲酸(ATRA)對(duì)高糖誘導(dǎo)HK-2細(xì)胞外基質(zhì)合成的影響及其在延緩糖尿病腎病腎間質(zhì)纖維化中的可能作用機(jī)制。方法將體外培養(yǎng)的HK-2細(xì)胞隨機(jī)分為七組:A組(5.5 mmol/L D-葡萄糖)、B組(30 mmol/L D-葡萄糖)、C組(5.5 mmol/L D-葡萄糖+24.5 mmol/L甘露醇)、D組(10~(-7)mol/L ATRA+30 mmol/L高糖)、E組(10~(-6)mol/L ATRA+30 mmol/L高糖)、F組(10~(-5)mol/L ATRA+30 mmol/L高糖)、G組(10μmol/L Y-27632+30 mmol/L高糖),各組細(xì)胞均培養(yǎng)48 h。應(yīng)用ELISA法檢測(cè)細(xì)胞培養(yǎng)液結(jié)締組織生長(zhǎng)因子(CTGF)和Ⅳ型膠原的表達(dá)水平;RT-PCR法檢測(cè)細(xì)胞Rho A、ROCK1的表達(dá)水平。結(jié)果 B組Rho A mRNA及ROCK1 mRNA的表達(dá)較A組顯著升高(P0.05),D、E、F組Rho A mRNA及ROCK1 mRNA較B組均表達(dá)減少(P0.05),且隨著ATRA濃度的升高呈現(xiàn)劑量依賴性。G組Rho A mRNA的表達(dá)與B組無(wú)明顯差異,但ROCK1 mRNA的表達(dá)較B組顯著減少(P0.05);直線相關(guān)分析顯示高糖組,D、E、F組Rho A mRNA與ROCK1 mRNA表達(dá)呈正相關(guān)(r=0.854,P=0.030;r=0.895,P=0.016;r=0.883,P=0.020;r=0.867,P=0.025);ELISA結(jié)果顯示D、E、F組及G組CTGF、Ⅳ型膠原表達(dá)較B組顯著下降(P0.05),且下降程度與ATRA濃度呈現(xiàn)劑量相關(guān)性。結(jié)論 ATRA在一定濃度范圍內(nèi)可抑制高糖誘導(dǎo)的HK-2細(xì)胞外基質(zhì)的合成,延緩腎間質(zhì)纖維化,其機(jī)制可能與抑制Rho A/ROCK通路有關(guān)。
[Abstract]:Objective to investigate the effect of ATRAA on the synthesis of extracellular matrix of HK-2 induced by high glucose and its possible mechanism in delaying renal interstitial fibrosis of diabetic nephropathy. Methods HK-2 cells cultured in vitro were randomly divided into seven groups: 1: a group (5. 5 mmol/L / L) and 5. 5 mmol/L / D group (n = 30 mmol/L). C group: 5. 5 mmol/L D-glucose 24. 5 mmol/L mannitol group. Group D: 10 mmol/L -7 mol / L ATRA 30 mmol/L high glucose group 10 -6 mol / L ATRA 30 mmol/L high glucose group F group 10 ~ (-5) mol / L ATRA ~ (-30) -5 mol / L / L ATRA 30 mmol/L. In group G, 10 渭 mol/L Y-27632 30 mmol/L high glucose content was observed, and the cells were cultured for 48 h. The expression level of connective tissue growth factor (CTGF) and type 鈪，

本文編號(hào)：1842250