發(fā)布時間：2018-05-02 03:11

  本文選題：氟伐他汀 + 腹膜透析��； 參考：《南京醫(yī)科大學》2014年碩士論文

【摘要】：目的：探討氟伐他汀(fluvastatin, Flu)對腹膜透析大鼠腹膜組織的保護作用。 方法：通過每日規(guī)律腹腔注射2.5%或4.25%濃度的葡萄糖腹透液建立腹膜透析大鼠模型。將42只SD雄性大鼠(體質量180-200g)隨機分為7個組：(1)正常對照組；(2)生理鹽水組：每日往大鼠腹腔注射100ml/kg生理鹽水；(3)單純Flu組：每日灌入10mg/kg氟伐他��；(4)2.5%腹透液組：每日往大鼠腹腔注射100ml/kg2.5%葡萄糖腹透液；(5)4.25%腹透液組：每日往大鼠腹腔注射100ml/kg4.25%葡萄糖腹透液；(6)Flu干預的2.5%腹透液組：每日往大鼠腹腔注射100ml/kg2.5%葡萄糖腹透液并同時灌入10mg/kg氟伐他��；(7)Flu干預的4.25%腹透液組：每日往大鼠腹腔注射100ml/kg4.25%葡萄糖腹透液并同時灌入10mg/kg氟伐他汀。6周后留取大鼠壁層腹膜組織,光鏡觀察各組大鼠壁層腹膜形態(tài)學的變化,同時采用RT-PCR法及免疫組化法(immunohistochemical, IHC)分別測定各組大鼠壁層腹膜分泌的轉化生長因子-β1(transforming growth factor,TGF-β1)及纖維連接蛋白(fibronectin, FN)的mRNA及蛋白表達量。 結果：光鏡下HE染色和Masson染色均顯示4.25%腹透液組與2.5%腹透液組大鼠腹膜較正常對照組明顯增厚；Flu干預的不同濃度腹透液組的壁層腹膜厚度薄于相應未干預的葡萄糖腹透液組,但仍厚于正常對照組；生理鹽水組和氟伐他汀組大鼠腹膜的厚度較正常對照組未見明顯改變。RT-PCR及IHC顯示2.5%腹透液組與4.25%腹透液組的TGF-β1和FN的mRNA及蛋白的表達量均明顯高于正常對照組(均P0.05), Flu干預的2.5%腹透液組的TGF-β1和FN的mRNA及蛋白的表達量較無Flu干預的2.5%葡萄糖腹透液組有顯著減少(均P0.05),Flu干預的4.25%腹透液組的TGF-β1和FN的mRNA及蛋白的表達量較無Flu干預的4.25%腹透液組亦有顯著減少(均P0.05),生理鹽水組和氟伐他汀組大鼠腹膜TGF-β1和FN的mRNA及蛋白表達量較正常組無明顯統(tǒng)計學意義(均P0.05)。 結論：氟伐他汀能改善高糖腹透液環(huán)境下大鼠腹膜的厚度,同時降低高糖腹透液誘導的大鼠腹膜TGF-β1和FN mRNA及蛋白的表達,對腹膜有保護作用。
[Abstract]:Aim: to investigate the protective effect of fluvastatin fluvastatin (Fluvastatin) on peritoneal tissue in peritoneal dialysis rats. Methods: peritoneal dialysis rat model was established by intraperitoneal injection of 2.5% or 4.25% glucose peritoneal dialysis solution. Forty-two Sprague-Dawley male rats (body mass 180-200g) were randomly divided into 7 groups: control group (n = 7) normal control group (n = 2) normal saline group: intraperitoneal injection of 100ml/kg saline to rats daily (n = 3) Flu group: daily administration of 10mg/kg fluvastatin and 42.5% peritoneal dialysate group: Rats were injected intraperitoneally with 100ml / kg 2.5% glucose peritoneal dialysate 54.25% peritoneal dialysate group: rats were injected 100 ml / kg 4.25% glucose peritoneal dialysate group with 2.5% Flu daily intraperitoneal injection of 100ml / kg 2.5% glucose peritoneal dialysate and simultaneously 10mg/kg fluvastation. The rats were given intraperitoneal injection of 100 ml / kg 4.25% glucose peritoneal dialysate daily with 10mg/kg fluvastatin at the same time for 6 weeks, and the peritoneal tissue of the rat wall layer was taken after the injection of fluvastatin at the same time for 6 weeks, in the group of 4.25% peritoneal dialysate, the rats were given intraperitoneal injection of 100 ml / kg 4.25% glucose per day. The morphologic changes of parietal peritoneum were observed under light microscope. The expression of transforming growth factor-尾 1(transforming growth factor- TGF- 尾 1 and fibronectin (FNs) in parietal peritoneum of rats in each group were measured by RT-PCR method and immunohistochemical method respectively. Results: under light microscope, HE staining and Masson staining showed that the peritoneal thickness of peritoneal layer of rats in 4.25% peritoneal dialysate group and 2.5% peritoneal dialysis group was significantly thicker than that in the control group treated with different concentrations of Flu, and the thickness of the parietal peritoneum in the peritoneal dialysate group with different concentrations was thinner than that in the corresponding glucose peritoneal dialysate group. But it was still thicker than the normal control group. The thickness of peritoneal membrane in saline group and fluvastatin group was not significantly changed compared with normal control group. RT-PCR and IHC showed that the expression of TGF- 尾 1 and FN mRNA and protein in 2.5% peritoneal dialysis group and 4.25% peritoneal dialysis group were significantly higher than those in normal control group (P < 0.05). The expression of mRNA and protein of TGF- 尾 1 and FN in 2.5% peritoneal dialysis group treated with Flu was significantly lower than that in 2.5% glucose peritoneal dialysis group without Flu intervention. The mRNA and protein expression of TGF- 尾 1 and FN in peritoneal membrane of rats in saline group and fluvastatin group were significantly lower than those in control group (all P 0.05). Conclusion: fluvastatin can improve the peritoneal thickness and decrease the expression of TGF- 尾 1 and FN mRNA and protein induced by high glucose peritoneal dialysis in rats.
【學位授予單位】：南京醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R692.5

【參考文獻】

相關期刊論文 前1條

1 Wei Cui;Han Zhang;Zhe-Li Liu;;Interleukin-6 receptor blockade suppresses subretinal fibrosis in a mouse model[J];International Journal of Ophthalmology(English Edition);2014年02期

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本文編號：1832130