本文選題：足細(xì)胞 + 上皮細(xì)胞-間充質(zhì)細(xì)胞轉(zhuǎn)分化��； 參考：《青島大學(xué)》2014年碩士論文

【摘要】：目的通過觀察高糖環(huán)境下足細(xì)胞snaill表達(dá)變化及骨形態(tài)發(fā)生蛋白-7(BMP-7)干預(yù)對(duì)高糖刺激足細(xì)胞p-smad2/3、snaill表達(dá)和足細(xì)胞EMT的影響,探討snaill在足細(xì)胞EMT中的作用及BMP-7抑制高糖誘導(dǎo)足細(xì)胞EMT的可能機(jī)制。 方法以體外培養(yǎng)的永生化小鼠足細(xì)胞(MPC5)為研究對(duì)象,分別給予不同時(shí)間(6h、12h、24h、48h)高糖刺激；將足細(xì)胞隨機(jī)分為以下4組：正常糖組(NG組)、高糖刺激組(HG組)、高糖+BMP-7干預(yù)組(BMP-7組)和甘露醇對(duì)照組(MG組),各組培養(yǎng)時(shí)間均為48h；采用倒置相差顯微鏡觀察各組足細(xì)胞形態(tài),采用實(shí)時(shí)熒光定量RT-PCR檢測(cè)足細(xì)胞snaill、nephrin、α-平滑肌肌動(dòng)蛋白(a-SMA) mRNA表達(dá)量,采用western(?)跡法檢測(cè)足細(xì)胞snail、p-smad2/3、nephrin、α-SMA蛋白表達(dá)量。 結(jié)果與NG組相比,高糖刺激可以使足細(xì)胞形態(tài)發(fā)生明顯改變,在轉(zhuǎn)錄水平和蛋白水平誘導(dǎo)snail、α-SMA的表達(dá)、減少nephrin的表達(dá)(P0.05),且隨著刺激時(shí)間的延長(zhǎng),作用更加明顯；與HG組相比,給予BMP-7干預(yù)在一定程度上可以逆轉(zhuǎn)高糖誘導(dǎo)的足細(xì)胞形態(tài)改變及p-smad2/3、snail、α-SMA的表達(dá)異常,增加足細(xì)胞nephrin表達(dá)(P0.05)；NG組與MG組足細(xì)胞各因子表達(dá)水平的差異無統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論高糖刺激可以呈時(shí)間依賴性上調(diào)足細(xì)胞snaill表達(dá),誘導(dǎo)足細(xì)胞轉(zhuǎn)分化；BMP-7可能通過拮抗TGFβ/smad通路調(diào)節(jié)高糖誘導(dǎo)足細(xì)胞snaill的異常表達(dá),抑制足細(xì)胞轉(zhuǎn)分化。
[Abstract]:Objective to observe the changes of snaill expression in podocytes and the effects of bone morphogenetic protein-7 BMP-7 on the expression of p-smad2 / 3nsnaill and podocyte EMT in podocytes stimulated by high glucose.To investigate the role of snaill in podocyte EMT and the possible mechanism of BMP-7 inhibiting high glucose induced podocyte EMT.Methods the immortalized mouse podocytes (MPC5) were cultured in vitro and were stimulated with high glucose at different time (6 h, 12 h, 24 h, 48 h).Podocytes were randomly divided into the following four groups: normal glucose group (NG group), high glucose stimulation group (HG group), high glucose BMP-7 intervention group (BMP-7 group) and mannitol control group (MG group) for 48 h.The expression of snaillonnephrin (偽 -smooth muscle actin) mRNA in podocytes was detected by real-time fluorescence quantitative RT-PCR.The expression of snaila p-smad 2 / 3 nephrin, 偽 -SMA protein in podocytes was detected by trace method.Results compared with NG group, high glucose stimulation could significantly change the morphology of podocyte, induce Snail, 偽 -SMA expression at transcription and protein level, and decrease the expression of nephrin P0.05, and the effect was more obvious with the prolongation of stimulation time, compared with HG group.The intervention of BMP-7 could reverse the morphological changes of podocytes induced by high glucose and the abnormal expression of p-smad2 / 3 snailand 偽 -SMA in podocytes to a certain extent, but there was no significant difference in the expression of podocytes between P0.05 and MG groups.Conclusion High glucose stimulation can up-regulate the expression of snaill in podocyte in a time-dependent manner. BMP-7 may regulate the abnormal expression of snaill in podocyte induced by high glucose by antagonizing the TGF 尾 -smad pathway and inhibiting the transdifferentiation of podocyte.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R692

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 柏鳳;俞偉男;聞萍;王曉華;方麗;曹紅娣;楊俊偉;譚若蕓;;高糖誘導(dǎo)足細(xì)胞發(fā)生上皮-間葉細(xì)胞轉(zhuǎn)分化[J];中華腎臟病雜志;2009年11期

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本文編號(hào)：1771507