本文選題：高脂血癥 + 飲酒��； 參考：《桂林醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的肥胖、高脂血癥、心腦血管疾病等已嚴(yán)重威脅人類(lèi)健康，本研究通過(guò)對(duì)家兔灌酒及高脂喂飼來(lái)制備家兔的高脂血癥模型，用以揭示不同白酒灌服量對(duì)高脂飲食新西蘭兔的血脂、血糖及腎功能的影響。 方法將36只新西蘭兔隨機(jī)分為6組，每組6只。A組：飼以普通顆粒飼料；B組：飼以高脂飼料（配方：1%膽固醇，7.5%蛋黃粉，3%豬油，88.5%基礎(chǔ)飼料）；C組：飼以普通顆粒飼料+白酒1.5g/kg·d；D組：予高脂喂養(yǎng)及白酒0.3g/kg·d；E組：予高脂喂養(yǎng)及白酒0.6g/kg·d；F組：予高脂喂養(yǎng)及白酒1.5g/kg·d。每兔每日進(jìn)食量為120g，酒量均分2次灌服，2次間隔8h，單籠喂養(yǎng)、飲水不限。各組家兔維持飲食和灌酒連續(xù)12周。分別于實(shí)驗(yàn)開(kāi)始前（0周）、第4、8、12周末留取全血、尿標(biāo)本，觀察家兔血清中葡萄糖(GLU)、總膽固醇(TCH)、甘油三酯(TG)、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、尿素氮（BUN）、肌酐（Cr）和尿β2微球蛋白（β2MG）的含量變化。于12周末處死新西蘭兔取腎，通過(guò)HE、Masson染色觀察判斷腎小球和腎小管的病理變化。 結(jié)果1)血脂、血糖水平：不同濃度飲酒均能降低高脂飲食家兔血清甘油三酯TG；低濃度飲酒能輕度降低低密度脂蛋白LDL（P＜0.05）；中濃度飲酒第4周、第8周低密度脂蛋白LDL升高，第12周低密度脂蛋白LDL下降（P＜0.05）；高濃度飲酒的低密度脂蛋白LDL明顯升高，高密度脂蛋白HDL早期升高后期降低（P＜0.05）；大量飲酒和高脂飲食均顯著升高血糖（P＜0.01）。2)腎功能：?jiǎn)渭兏咧嬍澈蛦渭冿嬀萍彝媚颚?微球蛋白均升高（P＜0.05），BUN和Cr未見(jiàn)顯著變化；高脂飲食+不同濃度飲酒使BUN、Cr和β2MG均升高（P＜0.05），，且升高幅度與飲酒濃度和時(shí)間成正相關(guān)（P＜0.05）。3)腎組織病理檢查：各組腎小球硬化系數(shù)比較，C組與A組差別無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05），B、D、E、F組均顯著高于A組（P＜0.05或P＜0.01）；與B組相比較，C組和D組無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05），E組和F組明顯高于B組（P＜0.05或P＜0.01）；與C組比較，D、E、F組顯著增高（P＜0.05或P＜0.01）。各組腎小管損傷分?jǐn)?shù)比較，B、C、D、E、F組均顯著高于A組（P＜0.01），與B組相比較， B、C、D、E、F組均明顯增高（P＜0.01）；與C組比較，D、E、F組顯著增高（P＜0.05或P＜0.01）。 結(jié)論適度飲酒能降低高脂飲食所致高脂血癥，大量飲酒和高脂飲食導(dǎo)致脂質(zhì)代謝紊亂、血糖升高及腎功能損傷。長(zhǎng)時(shí)間單純飲酒與單純高脂飲食也會(huì)導(dǎo)致腎臟組織損傷，在高脂飲食基礎(chǔ)上的不同程度飲酒會(huì)加重腎臟組織損害，而且隨飲酒量增加其腎臟損傷嚴(yán)重程度明顯增加，可導(dǎo)致嚴(yán)重的腎間質(zhì)纖維化和腎小球硬化
[Abstract]:Objective Obesity, hyperlipidemia, cardiovascular and cerebrovascular diseases have seriously threatened human health. In this study, the hyperlipidemia model of rabbits was established by drinking and feeding hyperlipidemia.The effects of different doses of liquor on blood lipids, blood glucose and renal function of New Zealand rabbits with high fat diet were studied.Methods 36 New Zealand rabbits were randomly divided into 6 groups.Group A: normal pellet feed group B: fed with high fat diet (1% 1% cholesterol, 7.5% egg yolk powder, 3% lard), group C: 1.5g/kg D group: high fat diet and white wine 0.3g/kg dai group: high fat feeding and liquor 0.6g/kg d F group: high fat feeding and liquor 1.5g/kg d.The amount of food consumed was 120 g per day, and the average amount of alcohol was divided into 2 times, 2 times interval 8 hours, single cage feeding, drinking water unlimited.The rabbits in each group were fed and perfused for 12 weeks.The whole blood and urine samples were collected at the end of the 4th week and the 12th week before the beginning of the experiment.The changes of serum glucose, TCHG, TGG, LDLX, HDLX, bun, Cr and 尾 2 microglobulin (尾 2MGG) in serum of rabbits were observed.At the end of 12 th week, New Zealand rabbits were sacrificed and the pathological changes of glomeruli and renal tubules were observed by HE Masson staining.Results 1) Serum lipids and blood glucose levels: different concentrations of alcohol drinking could decrease serum triglyceride level in rabbits with high fat diet, low concentration alcohol drinking could slightly reduce LDL(P of LDL < 0.05, moderate concentration of alcohol drinking could increase the level of LDL LDL at the 4th week and the 8th week after drinking.Low density lipoprotein (LDL) decreased at the 12th week (P < 0.05), and the low density lipoprotein (LDL) increased significantly in high alcohol drinking.High density lipoprotein (HDL) HDL decreased P < 0.05 in the early stage and increased significantly in both heavy drinking and high-fat diet (P < 0.01). Renal function was significantly increased in both high fat diet and drinking alone. No significant changes were found in urinary 尾 2-microglobulin (P < 0.05 bun and Cr) in rabbits with high fat diet and drinking alone.Drinking alcohol at different concentrations of high-fat diet increased bun Cr and 尾 2MG (P < 0.05), and the amplitude of increase was positively correlated with drinking concentration and time (P < 0.05) pathological examination of renal tissue: there was no significant difference in glomerulosclerosis coefficient between group C and group A (P > 0.05).There was no significant difference between group C and group D (P > 0.05) and group F (P < 0.05 or P < 0.01), and compared with group C (P < 0.05) and group D (P < 0.01), group D was significantly higher than group C (P < 0.05 or P < 0.01).The scores of renal tubule injury in group B were significantly higher than those in group A (P < 0.01), and those in group B were significantly higher than those in group B (P < 0.05 or P < 0.01) and in group C (P < 0.05 or P < 0.01).Conclusion moderate drinking can reduce hyperlipidemia induced by high fat diet, heavy drinking and high fat diet lead to disorder of lipid metabolism, increase of blood glucose and damage of renal function.Drinking alcohol for a long time and high fat diet for a long time would also lead to renal tissue injury, and different degrees of drinking on the basis of high fat diet would aggravate the renal tissue damage, and the severity of renal injury would increase with the increase of alcohol consumption.Can lead to severe interstitial fibrosis and glomerulosclerosis
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R589.2;R692

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