本文選題：糖尿病　切入點(diǎn)：勃起功能障礙　出處：《吉林大學(xué)》2014年碩士論文

【摘要】：目的：研究代謝記憶對(duì)于陰莖勃起功能障礙（ED）的影響及胰島素聯(lián)合ALT-711對(duì)糖尿病陰莖勃起功能相關(guān)代謝記憶的效果。 方法：將50只成年（8周齡）SD大鼠隨機(jī)分成5組：正常對(duì)照組（C）、糖尿病對(duì)照組（D）、胰島素治療組（D+I）、ALT-711治療組（D+ALT-711）和胰島素聯(lián)合ALT-711治療組（D+I+ALT-711）。一次性腹腔注射60mg/kg鏈脲霉素（streptozotocin,STZ）誘導(dǎo)糖尿病。誘導(dǎo)糖尿病8周后開始治療，ALT-711每天腹腔注射一次（3mg/kg）。2-6個(gè)單位的中效精蛋白胰島素一天兩次皮下注射，將胰島素治療組與胰島素聯(lián)合ALT-711治療組大鼠血糖控制在正常水平。連續(xù)治療6周，通過(guò)電刺激海綿體神經(jīng)測(cè)得海綿竇內(nèi)壓力（intracavernous pressures,ICP）評(píng)估勃起功能。測(cè)定糖基化終末產(chǎn)物（AGEs）及其受體（RAGE）表達(dá)、SOD活性、脂質(zhì)氧化產(chǎn)物。另外測(cè)定陰莖海綿體平滑肌、內(nèi)皮細(xì)胞及凋亡活動(dòng)等組織學(xué)改變。主要指標(biāo)有：ICP/MAP、陰莖海綿體內(nèi)皮細(xì)胞、平滑肌細(xì)胞、nNOS、AGE及其受體RAGE表達(dá)量、MDA含量、SOD活性及凋亡指數(shù)。 結(jié)果：糖尿病大鼠ICP/MAP比例、陰莖海綿體內(nèi)皮細(xì)胞、平滑肌細(xì)胞顯著降低，AGEs與RAGE表達(dá)顯著增高。單純胰島素與ALT-711治療只能使勃起功能與組織學(xué)變化部分改善。然而聯(lián)合治療組可以改善至類似于正常水平。單純ALT-711治療組相對(duì)于單純胰島素治療組更多的減少AGEs及其受體RAGE的沉積。 結(jié)論：研究表明，盡管胰島素可以有效的控制血糖水平，但是它不能完全的改善海綿體組織的病理變化。在嚴(yán)格控制血糖的基礎(chǔ)上聯(lián)合AGEs交聯(lián)蛋白裂解劑ALT-711可以得到更好的預(yù)期結(jié)果。聯(lián)合治療可能是通過(guò)抑制AGEs-RAGE-氧化應(yīng)激軸來(lái)消除代謝記憶。
[Abstract]:Aim: to study the effect of metabolic memory on erectile dysfunction (EDD) and the effect of insulin combined with ALT-711 on diabetic penile erectile dysfunction related metabolic memory. Methods: fifty adult SD rats of 8 weeks old were randomly divided into 5 groups: normal control group, diabetes control group, insulin treatment group, insulin treatment group and insulin combined with ALT-711 treatment group. 60mg/kg chain was injected intraperitoneally once. Diabetes induced by streptozotocinn (STZ). After 8 weeks of diabetes induction, the treatment of ALT-711 was started by intraperitoneal injection of 3 mg / kg 路kg. 2-6 units of midacting protamine insulin twice a day twice a day. The blood glucose of rats in insulin treatment group and insulin combined with ALT-711 group was controlled to normal level. The pressure in cavernous sinus was measured by electrical stimulation of cavernous nerve (ICP) to evaluate erectile function. The expression of superoxide dismutase (SOD) and lipid oxidation products were measured by glycation end product (ages) and its receptor (rage). The smooth muscle of cavernous body of penis was also measured. The main outcome measures were as follows: 1 ICP / MAPP, the expression of RAGE in cavernous endothelial cells and smooth muscle cells, the content of RAGE, the activity of SOD and the index of apoptosis. Results: the ratio of ICP/MAP and the endothelial cells of cavernosum of penis in diabetic rats, Smooth muscle cells decreased the expression of ages and RAGE significantly. Insulin and ALT-711 treatment only partially improved erectile function and histological changes. However, the combined treatment group could be improved to similar to the normal level. In the treatment group, the deposition of AGEs and its receptor RAGE was more reduced than that in the insulin alone group. Conclusion: studies have shown that, although insulin can effectively control blood glucose levels, However, it can not completely improve the pathological changes of cavernous tissue. The combination of AGEs crosslinking protein lysant ALT-711 on the basis of strict blood glucose control can achieve better expected results. The combined therapy may be by inhibiting the AGEs-RAGE-oxidation response. Excites the axis to eliminate metabolic memory.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R587.2;R698

【共引文獻(xiàn)】

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3 許t，

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