本文選題：冰溫　切入點(diǎn)：腎臟器官　出處：《天津商業(yè)大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：冰溫保存技術(shù)是近年來一種新的保存技術(shù)，本文以延長復(fù)雜生物器官保存時(shí)間為背景，利用生物、熱物理、臨床醫(yī)學(xué)等多學(xué)科相結(jié)合的方法，對(duì)生物組織冰溫保存過程中的熱質(zhì)傳遞過程進(jìn)行研究，研究內(nèi)容有組成生物體最基本的單位——細(xì)胞的低溫保存和整體器官的冰溫保存和移植，通過與臨床現(xiàn)行的保存方法進(jìn)行對(duì)比，在微觀層面和宏觀層面揭示冰溫延時(shí)保存技術(shù)應(yīng)用于復(fù)雜生物組織的傳熱傳質(zhì)機(jī)理。 本文以腎表皮細(xì)胞為研究對(duì)象，利用顯微鏡圖像分析方法，研究了其在不同溫度、不同滲透壓下的滲透特性，其中包括細(xì)胞的非滲透體積、細(xì)胞膜對(duì)水和低溫保護(hù)劑的滲透系數(shù)等。結(jié)果表明，在等滲溶液中，細(xì)胞的平均等滲體積Viso=2472.58μm3；非滲透體積Vb=0.22Viso；觀察細(xì)胞在高滲低溫保護(hù)劑溶液中的滲透響應(yīng)，利用兩參數(shù)模型，得到了不同溫度下在二甲基亞砜、乙二醇、甘油溶液中腎表皮細(xì)胞對(duì)水的滲透系數(shù)（Lp）、對(duì)低溫保護(hù)劑的滲透系數(shù)（Ps）及活化能（Ea）；通過對(duì)比細(xì)胞在冰溫和常溫下保存的存活率，得到了針對(duì)腎表皮細(xì)胞的最佳保存溫度。 在生物傳熱模型中，多孔介質(zhì)模型能夠描述腎臟組織的特征，多孔介質(zhì)的骨架由細(xì)胞構(gòu)成，而用于血液等體液流通的通道由毛細(xì)血管及淋巴管等構(gòu)成。在研究多孔介質(zhì)模型的過程中，需要將腎臟的物性進(jìn)行測(cè)定。本文對(duì)腎臟的兩項(xiàng)最基本的特性參數(shù)，即孔隙率和滲透率，進(jìn)行了研究。結(jié)果表明，豬腎臟的孔隙率為Φ=15.64%，滲透率為K=14.5μm2。該結(jié)果為腎臟冷灌注過程的數(shù)值模擬提供了實(shí)驗(yàn)基礎(chǔ)。 離體腎臟的冷灌注過程作為冰溫保存的首要環(huán)節(jié)，對(duì)該過程腎臟溫度場(chǎng)變化的研究具有重要的臨床價(jià)值，，在腎臟冷灌注過程中，對(duì)腎臟表面溫度和內(nèi)部溫度進(jìn)行了全面的監(jiān)控。在此實(shí)驗(yàn)基礎(chǔ)上，利用血管鑄型對(duì)腎臟的動(dòng)靜脈血管和腎臟外形進(jìn)行三維重建，得到腎臟的物理模型，并導(dǎo)入Fluent流體動(dòng)力模擬軟件中，以管內(nèi)層流流動(dòng)模型和腎臟組織多孔介質(zhì)模型相結(jié)合的方式，對(duì)腎臟冷灌注過程的三維溫度場(chǎng)進(jìn)行重構(gòu)。將模擬結(jié)果與實(shí)驗(yàn)結(jié)果對(duì)比，探索冷刺激作用下，灌注作用引起的腎臟溫度變化，并對(duì)溫度場(chǎng)模擬結(jié)果進(jìn)行了應(yīng)用研究。 最終，以上述研究為基礎(chǔ)，對(duì)豬進(jìn)行了自體移植臨床實(shí)驗(yàn)，將腎臟在-0.8℃下進(jìn)行36小時(shí)的冰溫保存后，重新植入體內(nèi)，移植后第四天，各項(xiàng)生理指標(biāo)恢復(fù)正常。該實(shí)驗(yàn)驗(yàn)證了冰溫保存方法可以抑制組織細(xì)胞的基礎(chǔ)代謝率，減少細(xì)胞的能量消耗。與傳統(tǒng)臨床保存方法相比，不僅保存溫度降低4-5℃，保存時(shí)間也從理論上16小時(shí)延長到實(shí)際可行的36小時(shí)，成為未來極具應(yīng)用前景的低溫保存方法。 本論文為國家自然科學(xué)基金資助項(xiàng)目（課題編號(hào):No.51076117）
[Abstract]:Ice temperature preservation technology is a new technology to save, the extension of complex biological preservation time as the background, the use of biological methods, thermal physics, clinical medicine and other multidisciplinary combination of biological tissue, heat and mass transfer during ice temperature preservation process was studied. The research contents are the basic composition of organisms unit cell cryopreservation and whole organ of ice temperature preservation and transplantation, compared with the current clinical preservation method, at the micro level and macro level of ice temperature preservation delaying technology applied to complex biological tissue heat and mass transfer mechanism.
In this paper, the renal epidermal cells as the research object, using the method of analysis of the microscope images, at different temperature, permeability under different osmotic pressure, including non permeable cell volume, cell membrane of water and cryoprotectant permeability coefficient. The results showed that in isotonic solution, the average cell etc. infiltration volume Viso=2472.58 M3; non penetration volume Vb=0.22Viso; observe cell in higher temperature protection penetrant solution in response to the two parameter model used at different temperatures are obtained in two dimethyl sulfoxide, ethylene glycol, the coefficient of permeability of renal epidermal cells in glycerin solution to water (Lp), permeability of the cryoprotective agent the (Ps) and activation energy (Ea); the survival rate by comparing the cells in ice temperature storage, obtained for renal epidermal cells the best storage temperature.
In the bio heat transfer model, porous media model can describe the characteristics of kidney tissue, porous skeleton composed of cells, and for blood and other body fluids circulation by capillary and lymphatic formation. In the process of porous media model, it is necessary to carry out the determination of physical properties of kidney. The two most basic the characteristic parameters of the kidney, the porosity and permeability were studied. The results show that the porosity of pig kidney is phi =15.64%, provides the experimental basis for K=14.5 m2. permeability of the results for the numerical simulation of the kidney cold perfusion process.
The first part from the cold perfusion process body kidney as ice temperature preservation, has important clinical value for studying the change of the temperature field in the process of kidney, kidney on renal cold perfusion process, surface temperature and internal temperature of comprehensive monitoring. On the basis of the experiment, 3D reconstruction of the renal artery and vein and the appearance of kidney using vascular casting, physical model of the kidney, and import the Fluent fluid dynamics simulation software, combined with pipe laminar flow model in porous media and kidney tissue model, to reconstruct the 3D temperature field of kidney cold perfusion process. The simulation results are compared with the experimental results, to explore the cold stimulation. The temperature change caused by the kidney, perfusion, and the simulation result of the temperature field was studied.
Finally, based on the above research, the clinical trials of autologous transplantation of pig kidney, will be at -0.8 deg.c for 36 hours after the ice temperature preservation, re implantation, fourth days after transplantation, normal physiological indexes. The experiment verifies the basic metabolism of ice temperature preservation method can suppress cell rate, reduce cellular energy consumption. Compared with the traditional clinical method of preservation, not only save reduces the temperature of 4-5 DEG C, extend the preservation time is theoretically feasible 16 hours to 36 hours, become the future promising cryopreservation method.
This paper is a project funded by the National Natural Science Foundation (project number: No.51076117)

【學(xué)位授予單位】：天津商業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：TB69;R692

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