本文選題：轉(zhuǎn)化生長因子β　切入點：smad蛋白　出處：《寧波大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：摘要：目的：探討腎移植后患者尿中smad2，4，7蛋白表達與慢性移植腎腎病（CAN）的關(guān)系，以期將檢測尿液中Smads蛋白表達作為一個無創(chuàng)檢測指標用于臨床。方法：實驗分為2組，，CAN組：腎移植術(shù)后發(fā)生CAN的患者30例；對照組：腎移植術(shù)后未發(fā)生CAN患者30例，留取各組研究對象的早晨中段尿液。用酶聯(lián)免疫技術(shù)（Elisa）檢測CAN組和對照組患者尿液中的Smad2、Smad4、Smad7的蛋白表達，用Real-Time PCR檢測CAN組和對照組患者尿液中的Smad2、Smad4、Smad7的mRNA表達。結(jié)果：經(jīng)Elisa檢測，CAN組患者尿液樣本中Smad2、 Smad4、Smad7與非CAN組患者水平的表達存在差異（p0.05）。RT-PCR結(jié)果顯示，CAN組患者尿液樣本中Smad2、Smad4的mRNA表達均明顯高于非CAN組患者，而非CAN組患者尿液樣本中Smad7的mRNA表達量顯著高于CAN組患者。（p0.05，具有統(tǒng)計學(xué)意義）。結(jié)論：通過我們的研究發(fā)現(xiàn)，CAN患者尿液中Smad2、Smad4的表達及含量均顯著高于非CAN患者組，而Smad7的表達及含量則顯著低于另一組，因此我們認為利用尿液Smad2、Smad4及Smad7聯(lián)合診斷CAN具有一定的臨床意義。這為我們找到了一個可以利用并且比較便捷的無創(chuàng)診斷慢性移植腎腎病的方法，從而為臨床醫(yī)生在臨床診斷中提供了非常具有價值的參考資料，也為門診動態(tài)觀察和隨訪腎移植受者提供了一個可靠的生物學(xué)指標。
[Abstract]:Abstract: objective: to investigate the relationship between the expression of Smad2O4M7 protein in urine and chronic renal allograft nephropathy (CAN) after renal transplantation. Methods: the patients were divided into two groups: 30 patients with CAN after renal transplantation, 30 patients without CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients without CAN after renal transplantation. The urine samples of each group were collected in the middle of the morning. The protein expression of Smad2 and Smad4 Smad7 in the urine of CAN group and control group was detected by enzyme-linked immunosorbent assay (Elisa). Real-Time PCR was used to detect the mRNA expression of Smad2, Smad4, Smad4, Smad7 in the urine of CAN group and control group. Results: there was a difference in the expression of Smad2, Smad4, Smad4, Smad7 between the CAN group and the non-#en5# group by Elisa. The results of RT-PCR showed that there was significant difference in urine samples of can group. The mRNA expression of Smad2 + Smad4 was significantly higher than that of non-#en1# patients. However, the mRNA expression of Smad7 in urine samples of patients without CAN was significantly higher than that of patients with CAN (P 0.05). Conclusion: the expression and content of Smad2 Smad4 in urine of patients with can are significantly higher than those of patients without CAN. The expression and content of Smad7 were significantly lower than those in the other group. Therefore, we think that the use of urine Smad2 Smad4 combined with Smad7 in the diagnosis of CAN is of certain clinical significance, which provides us with a more convenient and noninvasive method for the diagnosis of chronic renal allograft nephropathy. It provides a valuable reference for clinicians in clinical diagnosis and a reliable biological index for dynamic observation and follow-up of renal transplant recipients in outpatient clinic.
【學(xué)位授予單位】：寧波大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R699.2

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本文編號：1591090