本文選題：表柔比星(EPI)　切入點(diǎn)：茶多酚　出處：《細(xì)胞與分子免疫學(xué)雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的研究表柔比星(EPI)誘導(dǎo)人膀胱癌T24細(xì)胞發(fā)生自噬的分子機(jī)制,探討茶多酚(TP)聯(lián)合EPI對(duì)T24細(xì)胞的影響及其機(jī)制。方法將T24細(xì)胞分成對(duì)照組、EPI組、TP組、TP聯(lián)合EPI組。分別處理8 h,用透射電鏡觀察各組細(xì)胞自噬體形成情況,Western blot法檢測(cè)T24細(xì)胞中自噬相關(guān)蛋白微管相關(guān)蛋白輕鏈3Ⅱ(LC3Ⅱ)、p62水平。處理24 h后,用異硫氰酸熒光素標(biāo)記的膜聯(lián)素Ⅴ/碘化丙啶(annexinⅤ-FITC/PI)雙染色結(jié)合流式細(xì)胞術(shù)檢測(cè)各組細(xì)胞凋亡情況,Western blot法檢測(cè)T24細(xì)胞中活化的胱天蛋白酶3(c-caspase-3)、活化的多腺苷二磷酸核糖聚合酶(c-PARP)水平。用自噬通路抑制劑氯喹、3-甲基腺嘌呤聯(lián)合EPI處理T24細(xì)胞8 h,Western blot法檢測(cè)T24細(xì)胞LC3Ⅱ水平,再將TP與c-Jun氨基端激酶(JNK)通路抑制劑SP分別聯(lián)合EPI處理8 h后,檢測(cè)2組細(xì)胞LC3Ⅱ及磷酸化的JNK(p-JNK)水平。結(jié)果 TP聯(lián)合EPI組T24細(xì)胞自噬體數(shù)量及LC3Ⅱ含量明顯少于EPI組;JNK通路抑制劑SP能顯著降低EPI引起的LC3Ⅱ表達(dá),p-JNK水平與EPI呈時(shí)間依賴性增強(qiáng);TP聯(lián)合EPI能降低JNK通路活性;聯(lián)合組細(xì)胞凋亡率及c-caspase-3和c-PARP蛋白水平明顯高于EPI組。結(jié)論TP通過(guò)抑制JNK通路活性抑制膀胱癌T24細(xì)胞自噬并增強(qiáng)其對(duì)EPI敏感性。
[Abstract]:Objective to study the molecular mechanism of autophagy induced by epirubicin (EPI) in human bladder cancer T24 cells. To investigate the effect and mechanism of tea polyphenols (TP) combined with EPI on T24 cells. Methods T24 cells were divided into two groups: control group, TP group, TP group and EPI group. After 8 hours of treatment, the formation of autophagy in each group was observed by transmission electron microscope and detected by Western blot. The level of autophagy associated protein microtubule-associated protein light chain 3 鈪，

本文編號(hào)：1565536