發(fā)布時間：2018-03-03 12:03

  本文選題：代謝綜合征　切入點(diǎn)：內(nèi)質(zhì)網(wǎng)應(yīng)激　出處：《腎臟病與透析腎移植雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的:觀察小檗堿對代謝綜合征合并腎損害患者C/EBP同源蛋白(C/EBP homology protein,CHOP)mRNA、糖調(diào)節(jié)蛋白78(glucose-regulated protein 78,GRP78)mRNA表達(dá)和炎癥因子的影響,探討小檗堿對保護(hù)代謝綜合征合并腎損害的分子生物學(xué)機(jī)制。方法:收集2015年2月至2015年12月福州總醫(yī)院腎內(nèi)科臨床診斷符合代謝綜合征合并腎損害的患者20例,隨機(jī)分為治療組10例(給予鹽酸小檗堿300 mg,3次/d);對照組10例(采用常規(guī)西藥治療)。療程8周,觀察兩組治療前、治療4周、8周后尿微量白蛋白/肌酐比值(U-m Alb/C)、血清肌酐(SCr)及尿素氮(BUN)的變化。采用RT-PCR法檢測尿液中CHOP mRNA、GRP78 mRNA的表達(dá);采用ELISA法檢測血清中白細(xì)胞介素8(IL-8)、腫瘤壞死因子α(TNF-α)的含量。結(jié)果:治療8周后,對照組和治療組尿微量白蛋白/肌酐比值分別較治療前下降(P0.05)。與對照組比較,治療組尿微量白蛋白/肌酐比值下降更明顯(P0.05)。兩組治療前后SCr及BUN水平無顯著性差異。治療8周后,治療組尿液CHOP mRNA、GRP78 mRNA的表達(dá)及血清IL-8、TNF-α的含量均較治療前顯著降低(P0.05),對照組治療前后差異無統(tǒng)計(jì)學(xué)意義;與對照組比較,治療組尿液CHOP mRNA、GRP78 mRNA的表達(dá)及血清IL-8、TNF-α的含量均下降更明顯(P0.05)。結(jié)論:小檗堿可減少代謝綜合征合并腎損害患者的白蛋白尿,降低腎臟組織過度激活的內(nèi)質(zhì)網(wǎng)應(yīng)激反應(yīng),改善炎癥狀態(tài)。
[Abstract]:Objective: to observe the effects of berberine on the expression of C / EBP homology protein (C / EBP homology protein) and glucose-regulated protein 78 (GRP78) mRNA and inflammatory factors in patients with metabolic syndrome complicated with renal damage. To explore the molecular biological mechanism of berberine on the protection of metabolic syndrome with renal damage. Methods: from February 2015 to December 2015, 20 patients with metabolic syndrome complicated with renal damage were collected from the Department of Nephrology, Fuzhou General Hospital. They were randomly divided into treatment group (n = 10) and control group (n = 10) received berberine hydrochloride 300 mg / d for 3 times / d, and control group (n = 10) for 8 weeks. The changes of urinary microalbumin / creatinine ratio (U-m Alb / C), serum creatinine creatinine (SCR) and urea nitrogen bun (bun) were measured by RT-PCR method. The expression of CHOP mRNA-GRP78 mRNA in urine was detected by RT-PCR assay. The serum levels of IL-8 and TNF- 偽 in serum were determined by ELISA. Results: after 8 weeks of treatment, the urinary microalbumin / creatinine ratio in the control group and the treatment group was lower than that in the control group (P 0.05). The urinary microalbumin / creatinine ratio decreased more significantly in the treatment group than that in the control group (P 0.05). There was no significant difference in the levels of SCr and BUN between the two groups before and after treatment. After 8 weeks of treatment, there was no significant difference between the two groups. The expression of CHOP mRNA-GRP78 mRNA in urine and the content of serum IL-8 TNF- 偽 in the treatment group were significantly lower than those before and after treatment, but there was no significant difference between the control group and the control group. In the treatment group, the expression of CHOP mRNA-GRP78 mRNA in urine and the content of IL-8 TNF- 偽 in serum decreased significantly (P 0.05). Conclusion: berberine can reduce albuminuria and endoplasmic reticulum stress response in patients with metabolic syndrome and renal damage. Improve inflammation.
【作者單位】： 福州總醫(yī)院腎內(nèi)科福建醫(yī)科大學(xué)�？偱R床醫(yī)學(xué)院;
【基金】：國家自然基金面上項(xiàng)目(81373837) 福建省自然科學(xué)基金(2014J01371)
【分類號】：R589;R692
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本文編號：1560951