本文關(guān)鍵詞： 膀胱尿路上皮癌 缺氧誘導(dǎo)因子-1α E-鈣粘蛋白 波形蛋白 生存率　出處：《南昌大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的： 研究探討缺氧誘導(dǎo)因子-1α（hypoxia-inducible factor-1alpha,HIF-1α）和上皮間質(zhì)轉(zhuǎn)化（epithelial mesenchymal transition,EMT）相關(guān)蛋白在膀胱尿路上皮癌（urothelial carcinoma of the bladder,UCB）組織中的表達(dá)情況，探討相關(guān)蛋白表達(dá)與術(shù)后病理分期分級(jí)關(guān)系及與患者預(yù)后關(guān)系。 方法： 采用免疫組織化學(xué)法檢測(cè)97例膀胱尿路上皮癌組織中缺氧誘導(dǎo)因子-1α（HIF-1α）、E-鈣粘蛋白（E-cadherin）、波形蛋白（Vimentin）的表達(dá)情況。隨訪97例標(biāo)本所對(duì)應(yīng)的術(shù)后患者，主要隨訪術(shù)后患者并發(fā)癥發(fā)生情況包括近期并發(fā)癥、遠(yuǎn)期并發(fā)癥及生存狀況等，調(diào)查膀胱尿路上皮癌患者術(shù)后1年、2年、5年生存率。采用免疫組織化學(xué)法檢測(cè)5例正常膀胱組織中缺氧誘導(dǎo)因子-1α、E-鈣粘蛋白、波形蛋白的表達(dá)情況進(jìn)行對(duì)照。 結(jié)果： 選取97例行膀胱全切術(shù)住院患者手術(shù)切除標(biāo)本，病理證實(shí)為膀胱尿路上皮癌。分為：低級(jí)別尿路上皮癌（low-grade carcinoma,LGC）38例；高級(jí)別尿路上皮癌（high-grade carcinoma,HGC）59例。免疫組織化學(xué)法檢測(cè)結(jié)果示低級(jí)別尿路上皮癌（LGC）：缺氧誘導(dǎo)因子-1α：陽性率52.6%（20/38）、陰性率47.4%（18/38）。E-鈣粘蛋白：陽性率55.3%（21/38）、陰性率44.7%（17/38）；波形蛋白：陽性率36.8%（14/38）、陰性率63.2%（24/38）。高級(jí)別尿路上皮癌（HGC）：缺氧誘導(dǎo)因子-1α：陽性率72.9%（43/59）、陰性率27.1%（16/59）。E-鈣粘蛋白：陽性率32.2%（19/59）、陰性率67.8%（40/59）；波形蛋白：陽性率57.6%（34/59）、陰性率42.4%（25/59）。5例正常膀胱組織中免疫組織化學(xué)法檢測(cè)結(jié)果示：缺氧誘導(dǎo)因子-1α：陽性率0（0/5）、陰性率100%（5/5）；E-鈣粘蛋白：陽性率100%（5/5）、陰性率0（0/5）；波形蛋白：陽性率0（0/5）、陰性率100%（5/5）。 低級(jí)別尿路上皮癌組隨訪結(jié)果：患者38例，1例患者術(shù)后死于腎功能衰竭為非腫瘤死亡；2例患者失隨訪。隨訪結(jié)果統(tǒng)計(jì)如下：1年生存率75.0%（27/36）、2年生存率63.9%（23/36）、5年生存率41.7%（15/36）。高級(jí)別尿路上皮癌隨訪結(jié)果：患者59例，1例患者術(shù)后死于心功能衰竭，2例患者術(shù)后死于腎功能衰竭，1例患者術(shù)后死于感染導(dǎo)致的全身臟器功能衰竭，，均為非腫瘤死亡；7例患者失隨訪。隨訪結(jié)果統(tǒng)計(jì)如下：1年生存率71.2%（37/52）、2年生存率40.4%（21/52）、5年生存率13.5%（7/52）。 結(jié)論： 缺氧誘導(dǎo)因子-1α（HIF-1α）、E-鈣粘蛋白（E-cadherin）、波形蛋白（Vimentin）在膀胱尿路上皮癌的發(fā)生發(fā)展中發(fā)揮重要作用，隨著膀胱尿路上皮癌惡性程度增高，缺氧誘導(dǎo)因子-1α表達(dá)增高、E-鈣粘蛋白表達(dá)下降、波形蛋白表達(dá)增高。隨著膀胱尿路上皮癌惡性程度增高，患者1年、2年、5年生存率逐漸降低。缺氧誘導(dǎo)因子-1α與E-鈣粘蛋白、波形蛋白在膀胱尿路上皮癌發(fā)生發(fā)展及預(yù)后中發(fā)揮重要作用，可作為預(yù)后判斷指標(biāo)。
[Abstract]:Objective:. To investigate the expression of hypoxia-inducible factor-1 偽 hypoxia-inducible factor-1 alpha-HIF-1 偽 (HIF-1 偽) and epithelial mesenchymal transition (EMT-1 偽) in bladder urothelial carcinoma of the bladderderma, and to explore the relationship between the expression of HIF-1 偽 and postoperative pathological stages and prognosis. Methods:. The expression of hypoxia-inducible factor-1 偽 (HIF-1 偽) -E-cadherin (Vimentin) in 97 cases of bladder urothelial carcinoma was detected by immunohistochemical method. The main postoperative complications included short-term complications, long-term complications and survival status. The survival rate of 1 year, 2 years and 5 years after operation in patients with bladder urothelial carcinoma was investigated. The expression of hypoxia inducible factor-1 偽 -E-cadherin and vimentin in 5 normal bladder tissues were detected by immunohistochemical method. Results:. Ninety-seven inpatients with total cystectomy were selected and proved to be bladder urothelial carcinoma by pathology, including 38 cases of low-grade carcinomatous carcinoma of the urinary tract, and 38 cases of low-grade carcinoma of urinary tract. The results of immunohistochemical method showed that the low grade urothelial carcinoma (LUCC): hypoxia-inducible factor-1 偽: positive rate was 52.66% 20% 38%, negative rate was 47.4% and 1838% .E-cadherin: positive rate 55.33% 21 / 38%, negative rate 44.7T = 17 / 38%; vimentin: positive rate 36.88% 143838%; vimentin: positive rate 36.88% / 143838%; positive rate 55.338% .E-cadherin: positive rate 55.33% 21 / 38%, negative rate 44.7% 17 / 38%; vimentin: positive rate 36.88% 143838%; vimentin: positive rate 36.88% 143838%. High grade urothelial carcinoma HGCU: hypoxia inducible factor-1 偽: positive rate 72.9% 43 / 59%, negative rate 27.1%: positive rate 32.22% 1959%, negative rate 67.840% 40% 59%; vimentin: positive rate 57.66% / 3459%; negative rate 42.440% -59%; negative rate 42.440% 59%; negative rate 42.440% 59%; positive rate 37.840% 40% 59%; vimentin: positive rate 57.66% 3459%; negative rate 42.440% 59%; negative rate 42.440% 59%; negative rate 27.1%; positive rate 32.22% 1959%; negative rate 67.840% 40% 59%; vimentin: positive rate 57.66% 3459%; The results showed that the positive rate of hypoxia inducible factor-1 偽: positive rate 0 / 0 / 5%, negative rate 100% / 5 / 5% E-cadherin: positive rate 100% / 5%, negative rate 0% / 5%; vimentin: positive rate 0 / 0 / 5%, negative rate 100% / 5%. Follow-up results of low grade urothelial carcinoma group: one patient died of renal failure and 2 patients died of non-tumor death after operation. The results of follow-up were as follows: 1 year survival rate was 75.0% 27 / 36%, 2 years survival rate was 63.9% 23 / 36%, and 5 year survival rate was 41.775 / 36. Follow-up results of high grade urothelial carcinoma: one patient died of heart failure and two died of renal failure after operation. One patient died of systemic organ failure caused by infection after operation. The results of follow-up were as follows: 1 year survival rate was 71.2%, 37% / 52%, 2 year survival rate was 40.4% 21 / 52%, 5 year survival rate was 13. 5% / 52%. Conclusion:. Hypoxia-inducible factor-1 偽 (HIF-1 偽) plays an important role in the occurrence and development of bladder urothelial carcinoma. The expression of hypoxia inducible factor -1 偽 (HIF-1 偽) increases and the expression of E-cadherin decreases with the increase of malignancy of bladder urothelial carcinoma. The expression of vimentin increased. With the increase of malignant degree of bladder urothelial carcinoma, the survival rate of 1, 2 and 5 years decreased gradually, hypoxia inducible factor-1 偽 and E-cadherin, Ecadherin, hypoxia-inducible factor-1 偽 and E-cadherin, Vimentin plays an important role in the development and prognosis of bladder urothelial carcinoma and can be used as a prognostic index.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.14

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 黃江波;鄒飛燕;申鵬飛;羅志剛;齊琳;;HIF-1α、VEGF在膀胱移行細(xì)胞癌組織中的表達(dá)及其臨床意義[J];醫(yī)學(xué)臨床研究;2006年07期

2 董大海;趙軍;孫立江;張桂銘;;E鈣黏著素與波形蛋白在膀胱癌中的表達(dá)及意義[J];現(xiàn)代泌尿外科雜志;2010年01期

本文編號(hào)：1496827