本文關(guān)鍵詞： 前列腺癌 線粒體DNA 發(fā)病風險 預后　出處：《中南大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的：探索外周血白細胞的線粒體DNA (mtDNA)拷貝數(shù)與前列腺癌發(fā)病風險及預后的相關(guān)性。 方法：采用實時熒光定量PCR法檢測196例前列腺癌患者和196例年齡配對正常對照的外周血白細胞mtDNA拷貝數(shù),分析mtDNA拷貝數(shù)與前列腺癌發(fā)病風險的關(guān)系；隨訪其中89例前列腺癌患者,觀察兩個預后指標即疾病無進展生存時間、總生存時間,分析mtDNA拷貝數(shù)與腫瘤預后的關(guān)系。 結(jié)果：前列腺癌患者的外周血mtDNA拷貝數(shù)較正常對照的顯著升高(中位數(shù)0.91和0.82；P0.001)。mtDNA拷貝數(shù)與人群的前列腺癌發(fā)病風險顯著相關(guān),且兩者呈劑量反應關(guān)系(高四分位數(shù)vs低四分位數(shù)：OR=2.52，95%CI：1.35-4.70；Ptrend=0.011)。前列腺癌患者AJCC分期、Gleason評分越高,mtDNA拷貝數(shù)越高(P=0.002和P=0.012)。Cox單因素和多因素回歸模型提示mtDNA拷貝數(shù)是前列腺癌患者疾病進展和死亡預后的獨立危險因素。Kaplan-Meier生存分析顯示,mtDNA高拷貝數(shù)的前列腺癌患者與低拷貝數(shù)的相比,疾病無進展生存時間、總生存時間要短(P=0.007和P0.001)。 結(jié)論：外周血mtDNA拷貝數(shù)與前列腺癌發(fā)病風險顯著相關(guān)。在前列腺癌患者中,增高的mtDNA拷貝數(shù)與腫瘤的不良預后有關(guān)。量化外周血mtDNA拷貝數(shù)可能對前列腺癌發(fā)病風險評估和預后判斷有重要意義。
[Abstract]:Objective: to investigate the correlation between the mitochondrial DNA mtDNA copy number in peripheral blood leukocytes and the risk and prognosis of prostate cancer. Methods: the mtDNA copies of peripheral white blood leukocytes in 196 patients with prostate cancer and 196 age-matched normal controls were detected by real-time fluorescence quantitative PCR. To analyze the relationship between mtDNA copy number and the risk of prostate cancer. 89 patients with prostate cancer were followed up. Two prognostic indexes were observed: survival time without progression and total survival time. The relationship between mtDNA copy number and tumor prognosis was analyzed. Results: the number of mtDNA copies in peripheral blood of prostate cancer patients was significantly higher than that of normal controls (median 0.91 and 0.82respectively). The copy number of P0.001mtDNA was significantly correlated with the risk of prostate cancer in the population, and there was a dose-response relationship between them (high quartile vs low quartile: OR2.52). 95 CI: 1.35-4.70; AJCC staging and Gleason score were higher in patients with prostate cancer. The higher the copy number of mtDNA is, the higher P0. 002 and P0. 012 are. Cox univariate and multivariate regression models suggest that mtDNA copy number is an independent risk factor for disease progression and death prognosis in patients with prostate cancer. Kaplan-Meier survival analysis showed. Compared with low copy number prostate cancer patients with high copy number of mtDNA had no progressive survival time and the total survival time was shorter than that of P0. 007 and P0. 001. Conclusion: the number of mtDNA copies in peripheral blood is significantly associated with the risk of prostate cancer. The increased mtDNA copy number is related to the poor prognosis of the tumor. Quantifying the peripheral blood mtDNA copy number may play an important role in the risk assessment and prognosis of prostate cancer.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.25

【參考文獻】

相關(guān)期刊論文 前1條

1 韓蘇軍;張思維;陳萬青;李長嶺;;中國前列腺癌發(fā)病現(xiàn)狀和流行趨勢分析[J];臨床腫瘤學雜志;2013年04期

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本文編號：1467534