本文關(guān)鍵詞：C3a-C3aR在尿酸促進(jìn)人腎小管上皮細(xì)胞MCP-1表達(dá)中的作用　出處：《福建醫(yī)科大學(xué)學(xué)報(bào)》2015年06期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 腎小管上皮細(xì)胞 單核趨化蛋白- 尿酸

【摘要】：目的探討尿酸(UA)對(duì)人腎小管上皮細(xì)胞單核趨化蛋白-1(MCP-1)表達(dá)的影響及其機(jī)制。方法體外培養(yǎng)人腎皮質(zhì)近曲小管上皮細(xì)胞(HK-2),以不同濃度UA分別作用不同時(shí)間,利用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)檢測(cè)MCP-1的mRNA水平。在UA誘導(dǎo)下,通過(guò)針對(duì)補(bǔ)體C3的小干擾RNA(C3siRNA)轉(zhuǎn)染下調(diào)HK-2細(xì)胞內(nèi)源性C3的表達(dá)、小分子C3aR阻斷劑SB290157阻斷C3a-C3aR作用、外源性C3a刺激促進(jìn)C3aR激活3種方式抑制或激活C3aR的信號(hào)通路,并通過(guò)實(shí)時(shí)熒光定量PCR(Q-PCR)檢測(cè)其對(duì)于MCP-1mRNA表達(dá)的影響。結(jié)果 150μmol/L UA干預(yù)HK-2細(xì)胞12h可上調(diào)MCP-1mRNA轉(zhuǎn)錄水平;C3siRNA轉(zhuǎn)染或C3aR阻斷能夠抑制被UA上調(diào)的MCP-1mRNA的表達(dá);C3a與UA共同進(jìn)行干預(yù)時(shí),顯著增強(qiáng)UA對(duì)MCP-1mRNA的誘導(dǎo)作用。結(jié)論 UA可上調(diào)HK-2細(xì)胞MCP-1mRNA的表達(dá),其機(jī)制可能與UA激活C3或C3aR有關(guān)。
[Abstract]:Objective to investigate the serum uric acid (UA) on human renal tubular epithelial cells of monocyte chemoattractant protein -1 (MCP-1) expression and its influence mechanism. In HK-2 cell culture method in vitro (HK-2), with different concentrations of UA for different time respectively, using reverse transcriptase polymerase chain reaction (RT-PCR) to detect the level of mRNA MCP-1. Induced by UA, RNA by small interfering C3 (C3siRNA) to complement the down-regulation of HK-2 cell transfection and expression of endogenous C3, small molecule C3aR inhibitor SB290157 blocked C3a-C3aR effect of exogenous C3a stimulation C3aR signaling pathway activation in 3 ways to inhibit or activate C3aR, and real-time PCR (Q-PCR) detection and its effect on the expression of MCP-1mRNA. Results 150 mol/L UA intervention HK-2 cells 12h can upregulate the transcription level of MCP-1mRNA; C3siRNA or C3aR block can inhibit transfection upregulates the expression of UA MCP-1mRNA and C3a UA to intervene; The induction of MCP-1mRNA was significantly enhanced by UA. Conclusion UA can up regulate the expression of MCP-1mRNA in HK-2 cells, and the mechanism may be related to UA activation of C3 or C3aR.

【作者單位】： 福建醫(yī)科大學(xué)附屬第一醫(yī)院腎內(nèi)科;
【基金】：福建省衛(wèi)生廳青年科研課題(2012-1-25)
【分類(lèi)號(hào)】：R692
【正文快照】： (2015)06-0355-05慢性腎臟疾病中,趨化因子如單核趨化蛋白-1(monocyte chemotactic protein-1,MCP-1)在腎組織中的表達(dá)增加常常伴隨著腎臟纖維化的進(jìn)展[1],尿液中的MCP-1可以早期預(yù)測(cè)腎臟疾病[2]。補(bǔ)體C3片段C3a受體(compleneut 3areceptor,C3aR)是一個(gè)55kDa大小的蛋白質(zhì),主要

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