The type Ⅰ interferon and IFNAR play an important role in hepatitis B virus (HBV) infection and anti-HBV therapy. However, its mechanism of action is still poorly understood. To gain more insights into the role of typeⅠinterferon and typeⅠinterferon receptor (IFNAR) in HBV infection, we established an HBV persistent replication IFNAR knockout (IFNAR-/) mouse model and preliminarily applied this model. At first, the progeny of IFNAR-/mouse was reproduced. Then hydrodynamic injection with pAAV/HBV...

【文章頁數(shù)】：6 頁

【文章目錄】：
1 MATERIALS AND METHODS
    1.1 Animals
    1.2 Materials and Reagents
    1.3 Reproduction and Genotype Identification of IFNAR-/-Mice
    1.4 Establishment of Persistent HBV Replication Model in IFNAR-/-Mouse and Treatment with ETV
    1.5 Detection of Serum HBs Ag and HBe Ag
    1.6 Detection of Serum HBV DNA by Real-time PCR
    1.7 Immunohistochemical Analysis of Intrahepatic Hepatitis B Core Antigen (HBc Ag)
    1.8 Statistical Analysis
2 RESULTS
    2.1 Genotype Identification of the Offspring of IF-NAR-/-Mice
    2.2 The Persistence of the Serum HBs Ag, HBe Ag, HBV DNA and the Intrahepatic HBc Ag in HBV Replication IFNAR-/-Mice Model
    2.3 Effect of ETV Treatments on Serum HBs Ag andHBe Ag Expression, and HBV DNA Replication in Persistent HBV Replication IFNAR-/-or C57BL/6Mice Model
    2.4 Effect of ETV Treatments on HBc Ag Expressionin Liver Tissue in Persistent HBV Replication IF-NAR-/-or C57BL/6 Mice Model
3 DISCUSSION



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