【摘要】：目的探討妊娠期糖尿病(GDM)患者胎盤組織胰島素受體底物1(IRS-1)、蛋白酪氨酸磷酸酶1B(PTP1B)表達及意義。方法選擇GDM孕婦49例(GDM組),正常足月妊娠孕婦50例(正常組)。兩組均于孕24~28周檢測空腹胰島素(FINS)、空腹血糖(FPG),采用胰島素穩(wěn)態(tài)模型評估法(HOMA)計算胰島素抵抗指數(shù)(HOMAIR)、胰島β細胞功能(HOMA-β%)、胰島素敏感指數(shù)(HOMA-ISI);兩組分娩后取胎盤組織,采用免疫組化ABC法檢測胎盤組織IRS-1、PTP 1B。結(jié)果 GDM組FPG、FINS、HOMA-IR均高于正常組(P均0.01),HOMA-ISI、HOMA-β%均低于正常組(P均0.01)。正常組胎盤組織IRS-1陽性表達率為100%,GDM組為63.26%,兩組比較P0.01。正常組胎盤組織PTP 1B陽性表達率為56.00%,GDM組為91.83%,兩組比較P0.01。GDM患者胎盤組織PTP 1B與IRS-1表達呈負相關(r=-4.85,P0.01);IRS-1與HOMA-IR呈負相關(r=-0.552,P0.01),與HOMA-β%和HOMA-ISI無相關性(P均0.05);PTP 1B與HOMA-IR呈正相關(r=0.563,P0.01),與HOMA-β%和HOMA-ISI無相關性(P均0.05)。結(jié)論 GDM患者胎盤組織IRS-1表達降低,PTP 1B表達升高,且均與HOMA-IR相關;胰島素信號傳導系統(tǒng)中信號蛋白表達異�？赡芘cGDM發(fā)病有關。
[Abstract]:Objective to investigate the expression and significance of insulin receptor substrate 1 (IRS-1) and protein tyrosine phosphatase 1B (PTP1B) in placenta of patients with gestational diabetes mellitus (GDM). Methods 49 GDM pregnant women (GDM group) and 50 normal term pregnant women (normal group) were selected. Fasting insulin (FINS), fasting blood glucose (FPG), was measured at 24 脳 28 weeks of gestation. Insulin homeostasis model (HOMA) was used to calculate insulin resistance index (HOMAIR), islet 尾 cell function (HOMA- 尾%) and insulin sensitivity index (HOMA-ISI). Placental tissues were taken after delivery and placental tissue IRS-1,PTP 1B was detected by ABC method. Results FPG,FINS,HOMA-IR in GDM group was higher than that in normal group (P < 0.01), and HOMA-ISI,HOMA- 尾% was lower than that in normal group (P < 0.01). The positive expression rate of IRS-1 in placenta was 100% in normal group and 63.26% in GDM group. The positive expression rate of PTP 1B in placenta of normal group was 56.00%, and that of GDM group was 41.83%. There was a negative correlation between PTP 1B and IRS-1 expression in placenta of P0.01.GDM patients (r 鈮，

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