發(fā)布時(shí)間：2019-06-16 17:38

【摘要】：目的探討h TERC、ASPP2 mRNA在子宮內(nèi)膜癌變過(guò)程中的表達(dá)變化,并探討其臨床意義。方法收集正常子宮內(nèi)膜、子宮內(nèi)膜增生(單純?cè)錾�、�?fù)合增生、不典型增生)、子宮內(nèi)膜癌組織,分別采用熒光原位雜交法、半定量RT-PCR法檢測(cè)h TERC、ASPP2 mRNA,分析二者相關(guān)性及其與子宮內(nèi)膜癌臨床病理特征的關(guān)系。結(jié)果在正常子宮內(nèi)膜、子宮內(nèi)膜單純?cè)錾�、�?fù)合增生、不典型增生及子宮內(nèi)膜癌中h TERC mRNA表達(dá)依次升高、ASPP2mRNA表達(dá)依次降低,除h TERC在正常子宮內(nèi)膜與單純?cè)錾g、ASPP2 mRNA在不典型增生與子宮內(nèi)膜癌之間的表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義(P均0.05)外,其余兩兩比較差異均有統(tǒng)計(jì)學(xué)意義(P均0.05);不同子宮內(nèi)膜組織中h TERC、ASPP2 mRNA的表達(dá)呈負(fù)相關(guān)(r=-0.625,P0.05);h TERC、ASPP2 mRNA表達(dá)與子宮內(nèi)膜癌組織學(xué)分級(jí)、癌浸潤(rùn)深度、淋巴結(jié)轉(zhuǎn)移有關(guān)(P均0.05),h TERC mRNA表達(dá)與臨床分期無(wú)關(guān)(P0.05),ASPP2 mRNA表達(dá)與臨床分期有關(guān)(P0.05)。結(jié)論在子宮內(nèi)膜癌變過(guò)程中,h TERC mRNA表達(dá)上調(diào)、ASPP2 mRNA表達(dá)下調(diào)或缺失,二者有助于子宮內(nèi)膜癌的早期診斷和預(yù)后判斷。
[Abstract]:Objective to investigate the expression of h TERC,ASPP2 mRNA in endometrial carcinogenesis and its clinical significance. Methods the correlation between normal endometrium, intimal hyperplasia (simple hyperplasia, compound hyperplasia, atypical hyperplasia) and endometrial carcinoma was detected by fluorescence in situ hybridization and semi-quantitative RT-PCR, respectively. The correlation between them and the clinicopathological features of endometrial carcinoma was analyzed by semi-quantitative PCR. Results the expression of h TERC mRNA increased and the expression of ASPP2mRNA decreased in normal endometrium, simple hyperplasia, compound hyperplasia, atypical hyperplasia and endometrial carcinoma. Except for h TERC, there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and endometrial carcinoma, but there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and intimal carcinoma (P < 0.05), but there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and endometrial carcinoma (P < 0.05), but there was no significant difference in the expression of ASPP2mRNA between normal and simple hyperplasia (P < 0.05), but there was no significant difference between normal and simple hyperplasia (P < 0.05). The expression of h TERC,ASPP2 mRNA in different endometrial tissues was negatively correlated (r 鈮，

本文編號(hào)：2500703