【摘要】：目的檢測(cè)子宮肌瘤及瘤旁正常平滑肌組織中microRNA-18a(miR-18a)及雌激素受體α(ERα)的表達(dá)差異,探討microRNA-18a及ERα與子宮肌瘤的關(guān)系。方法用原位雜交法和免疫組化法分別檢測(cè)microRNA-18a和ERα在子宮肌瘤及瘤旁正常平滑肌組織石蠟切片中的表達(dá),并評(píng)價(jià)microRNA-18a及ERα的表達(dá)之間的關(guān)系。結(jié)果子宮肌瘤組織中microRNA-18a表達(dá)顯著低于瘤旁正常平滑肌組織(P0.05),而ERα表達(dá)顯著高于瘤旁正常平滑肌組織(P0.05)。子宮肌瘤組織及瘤旁正常平滑肌組織中microRNA-18a及ERα的表達(dá)量呈負(fù)相關(guān)。結(jié)論 microRNA-18a可能通過(guò)影響ERα在子宮肌瘤中的表達(dá),從而在子宮肌瘤發(fā)生發(fā)展過(guò)程中起到重大作用;microRNA-18a可能成為治療子宮肌瘤的新靶標(biāo)。
[Abstract]:Objective to detect the expression of microRNA-18a (miR-18a) and estrogen receptor 偽 (ER 偽) in uterine myoma and adjacent normal smooth muscle tissues, and to explore the relationship between microRNA-18a and ER 偽 and uterine myoma. Methods the expression of microRNA-18a and ER 偽 in paraffin sections of uterine myoma and normal smooth muscle tissue adjacent to tumor was detected by in situ hybridization and immunohistochemistry, respectively, and the relationship between the expression of microRNA-18a and ER 偽 was evaluated. Results the expression of microRNA-18a in uterine myoma was significantly lower than that in normal smooth muscle tissue adjacent to tumor (P 0.05), while the expression of ER 偽 in uterine myoma was significantly higher than that in normal smooth muscle tissue adjacent to tumor (P 0.05). There was a negative correlation between the expression of microRNA-18a and ER 偽 in uterine myoma and normal smooth muscle tissues adjacent to the tumor. Conclusion microRNA-18a may play an important role in the occurrence and development of uterine myoma by affecting the expression of ER 偽 in uterine myoma, and microRNA-18a may be a new target for the treatment of uterine myoma.
【作者單位】： 電子科技大學(xué)附屬醫(yī)院四川省人民醫(yī)院婦產(chǎn)科;電子科技大學(xué)附屬醫(yī)院四川省人民醫(yī)院藥學(xué)部;
【基金】：四川省衛(wèi)生廳基金資助項(xiàng)目(100491,120111) 四川省科技廳基金資助項(xiàng)目(2014FZ0103,2015JQO027,015ZR0160) 四川省人民醫(yī)院青年基金資助項(xiàng)目(30305030606,30305030859)
【分類(lèi)號(hào)】：R737.33
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本文編號(hào)：2499124