【摘要】：目的檢測血管內(nèi)皮生長因子(VEGF)、基質(zhì)金屬蛋白酶(MMP)-2和腫瘤壞死因子(TNF)-α在人子宮肌瘤中的表達,探討其在子宮肌瘤發(fā)生、發(fā)展的作用。方法取2013年1~12月該院收治的54例子宮肌瘤患者的子宮肌瘤組織和正常子宮肌組織,分別采用免疫組化法測定雌激素受體(ER)和孕激素受體(PR)的表達,采用RT-PCR法測定VEGF、MMP-2和TNF-αmRNA的表達,比較子宮肌瘤組織和正常子宮肌組織表達的差異,并且分析ER、PR與VEGF、MMP-2、TNF-α表達的相關性。結(jié)果 (1)子宮肌瘤組織ER和PR的陽性表達率分別為68.52%和75.93%,均顯著高于正常子宮肌組織(P0.05或P0.01);(2)子宮肌瘤組織中VEGF、MMP-2和TNF-αmRNA表達水平均較正常子宮肌組織顯著升高(P0.01);(3)ER(+)和PR(+)子宮肌瘤組織VEGF、MMP-2和TNF-αmRNA表達較ER(-)和PR(-)子宮肌瘤組織均顯著升高(P0.01);(4)相關性分析顯示,ER、PR表達評分均與VEGF、MMP-2和TNF-αmRNA表達水平呈正相關(P0.01)。結(jié)論 VEGF、MMP-2和TNF-α在子宮肌瘤均高表達,在其發(fā)生、發(fā)展中起重要作用,并且ER和PR的高表達相關。
[Abstract]:Objective to detect the expression of vascular endothelial growth factor (VEGF), matrix metalloprotease (MMP)-2 and tumor necrosis factor (TNF)-偽 in human uterine myoma and to explore the role of vascular endothelial growth factor (MMP)-2 and tumor necrosis factor (TNF)-偽 in the occurrence and development of uterine myoma. Methods from January to December 2013, the expressions of estrogen receptor (ER) and progesterone receptor (PR) were measured in 54 patients with uterine myoma and normal uterine muscle tissues. The expression of VEGF,MMP-2 and TNF- 偽 mRNA was detected by RT-PCR method, and the difference of expression between uterine myoma tissue and normal uterine muscle tissue was compared, and the correlation between ER,PR and VEGF,MMP-2,TNF- 偽 expression was analyzed. Results (1) the positive expression rates of ER and PR in uterine myoma were 68.52% and 75.93%, respectively, which were significantly higher than those in normal uterine muscle (P 0.05 or P 0.01). (2) the expression of VEGF,MMP-2 and TNF- 偽 mRNA in uterine myoma was significantly higher than that in normal uterine muscle (P 0.01). (3) the expression of VEGF,MMP-2 and TNF- 偽 mRNA in) ER () and PR () hysteromyoma tissues was significantly higher than that in ER (-) and PR (-) hysteromyoma tissues (P 0.01). (4) correlation analysis showed that ER,PR expression score was positively correlated with the expression of VEGF,MMP-2 and TNF- 偽 mRNA (P 0.01). Conclusion both VEGF,MMP-2 and TNF- 偽 are highly expressed in uterine myoma and play an important role in its occurrence and development, and the high expression of ER and PR is related.
【作者單位】： 唐山市開灤(集團)有限責任公司唐家莊醫(yī)院;開灤總醫(yī)院;
【基金】：河北省科學技術廳課題(20132924)
【分類號】：R737.33

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