【摘要】：目的： 通過在正常宮頸上皮組織、宮頸上皮內(nèi)瘤變(cervical intraepithelial neoplasia, CIN)以及宮頸鱗狀細胞癌(cervical squamous cell carcinoma, CSCC)中檢測Klotho和Wnt10b表達,旨在探索宮頸癌發(fā)生、發(fā)展過程中兩者所發(fā)揮的作用及二者之間的相關(guān)性。 方法： (1)采用免疫組化SP法檢測Klotho及Wnt10b在20例正常宮頸上皮組織、41例CIN組織及106例CSCC組織中的表達。 (2)使用實時熒光定量法(real-time PCR;RT-PCR)檢測5例正常宮頸組織、3例CIN組織以及7例CSCC組織中Klotho與Wnt10b的mRNA含量。 結(jié)果： (1)Klotho在正常宮頸上皮組織中的表達明顯高于CIN組織及CSCC組織,其在正常宮頸上皮組織、CIN組織及CSCC組織中的陽性表達率依次減低,差異有統(tǒng)計學意義(P0.05)。Wnt10b在CSCC組織中的表達明顯高于正常宮頸組織、CIN組織,其在正常宮頸上皮組織、CIN組織及CSCC組織中的陽性表達率依次上調(diào),差異有統(tǒng)計學意義(P0.05) (2) Klotho表達與宮頸鱗癌患者年齡無相關(guān)性(P0.05),與宮頸癌FIGO分期、組織學分化程度以及淋巴結(jié)轉(zhuǎn)移有關(guān),差異有統(tǒng)計學意義(P0.05)；Wnt10b表達與患者年齡無相關(guān)性(P0.05),與分化程度、FIGO分期及淋巴結(jié)轉(zhuǎn)移有關(guān),差異有統(tǒng)計學意義(P0.05)。 (3)Klotho mRNA在正常宮頸上皮組織中的表達顯著高于CSCC組織,Wnt10b mRNA在CSCC組織中的表達顯著高于正常宮頸組織。 (4)在CSCC組織中,Klotho蛋白與Wnt10b的蛋白表達呈負相關(guān)(rs=-0.416,P0.01)。 結(jié)論： (1) Klotho蛋白在正常宮頸組織、CIN組織及CSCC組織中的陽性表達率逐步降低,與宮頸鱗癌的分化程度、FIGO分期以及淋巴結(jié)轉(zhuǎn)移密切相關(guān),這表明Klotho在抑制宮頸癌的發(fā)生發(fā)展過程中發(fā)揮重要作用。 (2) Wnt10b在正常宮頸組織、CIN及CSCC組織中的陽性表達率依次增高,且與宮頸鱗癌的分化程度、FIGO分期以及淋巴結(jié)轉(zhuǎn)移等具有相關(guān)性,提示W(wǎng)nt10b在宮頸鱗癌的發(fā)生、發(fā)展、侵襲以及轉(zhuǎn)移中有著重要的作用。 (3)與正常宮頸粘膜組相比較,CSCC組織中Klotho mRNA含量減少,而Wnt10b mRNA含量增多,并且Klotho與Wnt10b蛋白表達呈負相關(guān),提示Klotho和Wnt10b可能在宮頸癌的發(fā)生、發(fā)展過程中起重要作用,聯(lián)合檢測Klotho和Wnt10b有助于宮頸癌的早期診斷。
[Abstract]:Objective: to detect the expression of Klotho and Wnt10b in normal cervical epithelium, cervical intraepithelial neoplasia (cervical intraepithelial neoplasia, CIN) and cervical squamous cell carcinoma (cervical squamous cell carcinoma, CSCC) in order to explore the carcinogenesis of cervical cancer. The role of the two in the process of development and the correlation between the two. Methods: (1) Immunohistochemistry SP method was used to detect the expression of Klotho and Wnt10b in 20 cases of normal cervical epithelium, 41 cases of CIN and 106 cases of CSCC. (2) the mRNA contents of Klotho and Wnt10b in 5 cases of normal cervical tissues, 3 cases of CIN tissues and 7 cases of CSCC tissues were detected by real-time fluorescence quantitative analysis (real-time PCR;RT-PCR). Results: (1) the expression of Klotho in normal cervical epithelial tissues was significantly higher than that in CIN and CSCC tissues, and the positive expression rates in normal cervical epithelial tissues, CIN tissues and CSCC tissues decreased in turn. The expression of Wnt10b in CSCC tissue was significantly higher than that in normal cervical tissue, CIN tissue, and the positive rate of WNT 10b in normal cervical epithelial tissue, CIN tissue and CSCC tissue was up-regulated in turn, and the expression of Wnt10b in normal cervical epithelial tissue, CIN tissue and CSCC tissue was up-regulated. The difference was statistically significant (P0.05) (2) the expression of Klotho was not correlated with the age of cervical squamous cell carcinoma (P0.05), but correlated with FIGO stage, histological differentiation and lymph node metastasis of cervical cancer. The difference was statistically significant (P0.05); The expression of Wnt10b was not correlated with age (P0.05), but correlated with the degree of differentiation, FIGO stage and lymph node metastasis (P0.05). (3) the expression of) Klotho mRNA in normal cervical epithelial tissues was significantly higher than that in CSCC tissues, and the expression of Wnt10b mRNA in CSCC tissues was significantly higher than that in normal cervical tissues. (4) in CSCC tissues, the expression of Klotho protein was negatively correlated with the expression of Wnt10b protein (rs=-0.416,P0.01). Conclusion: (1) the positive expression rate of Klotho protein in normal cervical tissues, CIN tissues and CSCC tissues decreased gradually, which was closely related to the differentiation degree, FIGO stage and lymph node metastasis of cervical squamous cell carcinoma. These results suggest that Klotho plays an important role in inhibiting the occurrence and development of cervical cancer. (2) the positive expression rate of Wnt10b in normal cervical tissues, CIN and CSCC tissues was increased in turn, and it was correlated with the differentiation degree, FIGO stage and lymph node metastasis of cervical squamous cell carcinoma, which suggested the occurrence and development of Wnt10b in cervical squamous cell carcinoma. Invasion and metastasis play an important role. (3) compared with the normal cervical mucosa group, the content of Klotho mRNA in CSCC tissue decreased, while the content of Wnt10b mRNA increased, and the expression of Klotho was negatively correlated with the expression of Wnt10b protein, suggesting that Klotho and Wnt10b might play an important role in the development of cervical cancer. The combined detection of Klotho and Wnt10b is helpful for the early diagnosis of cervical cancer.
【學位授予單位】：蘭州大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.33

【參考文獻】

相關(guān)期刊論文 前3條

1 樂飛;鄭民華;;Wnt/β-catenin信號轉(zhuǎn)導(dǎo)通路與結(jié)直腸癌[J];國際病理科學與臨床雜志;2006年03期

2 黃曙;湯小偉;林潔;范志寧;季國忠;;Klotho基因在胃癌組織中的表達及意義[J];世界華人消化雜志;2011年30期

3 許良中，，楊文濤;免疫組織化學反應(yīng)結(jié)果的判斷標準[J];中國癌癥雜志;1996年04期

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