紫花牡荊素對SKOV3卵巢癌干細(xì)胞樣細(xì)胞FoxO3a磷酸化的影響
發(fā)布時間：2019-04-19 09:13
【摘要】：背景與目的:腫瘤干細(xì)胞的存在是卵巢癌高復(fù)發(fā)率和高死亡率的原因。本文研究紫花牡荊素(casticin,CAS)對SKOV3細(xì)胞系卵巢癌干細(xì)胞樣細(xì)胞(ovarian cancer stem cell like cells,OCSLCs)自我更新能力的影響及其機(jī)制。方法:體外培養(yǎng)SKOV3細(xì)胞系細(xì)胞,干細(xì)胞培養(yǎng)基懸浮培養(yǎng)SKOV3得到和擴(kuò)增卵巢癌球形成細(xì)胞,即OCSLCs。蛋白[質(zhì)]印跡法(Western blot)分析FoxO3a磷酸化水平。FoxO3a特異性si RNA轉(zhuǎn)染抑制FoxO3a蛋白表達(dá),并測定腫瘤球形成率。結(jié)果:與親本細(xì)胞相比,OCSLCs高表達(dá)磷酸化FoxO3a蛋白,具有更高的腫瘤球形成率[(3.1±0.3)%vs(34.8±6.8)%,P0.05]。CAS顯著抑制OCSLCs腫瘤球形成能力,并降低FoxO3a磷酸化水平。FoxO3a特異性si RNA轉(zhuǎn)染抑制FoxO3a蛋白表達(dá),拮抗CAS抑制OCSLCs腫瘤球形成作用。結(jié)論:降低FoxO3a磷酸化水平參與CAS抑制SKOV3細(xì)胞系OCSLCs腫瘤球形成作用。
[Abstract]:Background & objective: the existence of tumor stem cells is the cause of high recurrence rate and high mortality of ovarian cancer. In this study, we studied the effect of casticin,CAS on the self-renewal ability of ovarian cancer stem cell-like cell line (ovarian cancer stem cell like cells,OCSLCs in SKOV3 cell line and its mechanism. Methods: SKOV3 cell line was cultured in vitro, and SKOV3 in suspension culture medium of stem cells was used to obtain and amplify ovarian cancer bulb forming cells, I. E., OCSLCs.. The phosphorylation level of FoxO3a was analyzed by (Western blot). The expression of FoxO3a protein was inhibited by FoxO3a-specific si RNA transfection, and the formation rate of tumor globules was determined. Results: compared with parental cells, OCSLCs expressed phosphorylated FoxO3a protein with a higher tumor globular formation rate [(3.1 鹵0.3)% vs (34.8 鹵6.8)%, P0.05]. Cas significantly inhibited the formation of OCSLCs tumor globules. FoxO3a-specific si RNA transfection inhibited the expression of FoxO3a protein and antagonized the inhibitory effect of CAS on the formation of OCSLCs tumor globules. Conclusion: lowering the phosphorylation level of FoxO3a is involved in the inhibitory effect of CAS on the formation of tumor globules in SKOV3 cell line OCSLCs.
【作者單位】： 湖南師范大學(xué)附屬第一醫(yī)院婦產(chǎn)科;湖南師范大學(xué)醫(yī)學(xué)院病理學(xué)教研室;
【基金】：湖南省教育廳科學(xué)研究一般項目(13C524)
【分類號】：R737.31
[Abstract]:Background & objective: the existence of tumor stem cells is the cause of high recurrence rate and high mortality of ovarian cancer. In this study, we studied the effect of casticin,CAS on the self-renewal ability of ovarian cancer stem cell-like cell line (ovarian cancer stem cell like cells,OCSLCs in SKOV3 cell line and its mechanism. Methods: SKOV3 cell line was cultured in vitro, and SKOV3 in suspension culture medium of stem cells was used to obtain and amplify ovarian cancer bulb forming cells, I. E., OCSLCs.. The phosphorylation level of FoxO3a was analyzed by (Western blot). The expression of FoxO3a protein was inhibited by FoxO3a-specific si RNA transfection, and the formation rate of tumor globules was determined. Results: compared with parental cells, OCSLCs expressed phosphorylated FoxO3a protein with a higher tumor globular formation rate [(3.1 鹵0.3)% vs (34.8 鹵6.8)%, P0.05]. Cas significantly inhibited the formation of OCSLCs tumor globules. FoxO3a-specific si RNA transfection inhibited the expression of FoxO3a protein and antagonized the inhibitory effect of CAS on the formation of OCSLCs tumor globules. Conclusion: lowering the phosphorylation level of FoxO3a is involved in the inhibitory effect of CAS on the formation of tumor globules in SKOV3 cell line OCSLCs.
【作者單位】： 湖南師范大學(xué)附屬第一醫(yī)院婦產(chǎn)科;湖南師范大學(xué)醫(yī)學(xué)院病理學(xué)教研室;
【基金】：湖南省教育廳科學(xué)研究一般項目(13C524)
【分類號】：R737.31
【共引文獻(xiàn)】
相關(guān)期刊論文 前10條
1 羅海清;劉付梅;崔仲宜;李祥勇;;鼻咽癌組織中WWOX基因和蛋白的表達(dá)及其臨床意義[J];廣東醫(yī)學(xué)院學(xué)報;2013年05期
2 厲碧榮;李R，
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