陰道內(nèi)乳桿菌對陰道加德納菌生物膜影響的初步研究
發(fā)布時間：2019-03-06 20:49
【摘要】：目的:研究陰道加德納菌(GV)生物膜體外生長過程及陰道來源乳桿菌對GV生物膜的影響。方法:選取GV標(biāo)準(zhǔn)菌株(ATCC14018)體外培養(yǎng)生物膜,在成膜的不同時期檢測生物膜生成量及觀察生物膜生成形態(tài)。選取陰道來源乳桿菌1#(鼠李糖乳桿菌)、2#(乳酪乳桿菌),分別在GV生物膜生成后的0h、24h、48h加等量乳桿菌共培養(yǎng)24h,用Live/Dead熒光染色劑染色,觀察GV生物膜生成情況。結(jié)果:(1)同一培養(yǎng)條件下,GV生物膜生成不同時期的OD值有明顯差異(P0.05)。電鏡掃描圖片顯示,培養(yǎng)4~20h時見散落的單菌落,24h時單獨菌落開始連接,48h時形成連接的菌落外面包裹一層胞外聚合物,48h后形態(tài)無變化。(2)GV生物膜的熒光染色顯示,陰道內(nèi)乳桿菌對抗生長后0h、24h時,GV生物膜厚度減少,形成松散的菌落,以乳桿菌為主;對抗生長后48h,菌落密度無變化,死菌面積增大,活菌以乳桿菌為主。結(jié)論:(1)陰道GV生物膜形成較緩慢,開始成膜時間約為培養(yǎng)后24h,約在48h后形成成熟的生物膜。(2)陰道內(nèi)乳桿菌可干擾GV生物膜的形成,不同乳桿菌對不同時期GV生物膜的作用不同。
[Abstract]:Aim: to study the growth process of Gardnerella vaginalis (GV) biofilm in vitro and the effect of Lactobacillus vaginalis on GV biofilm. Methods: GV standard strain (ATCC14018) was selected to culture biofilm in vitro. The biofilm formation and morphology of biofilm formation were observed at different stages of biofilm formation. Lactobacillus vaginalis 1 # (rhamnose Lactobacillus) and 2 # (Lactobacillus casei) were co-cultured for 24 hours at 0 h, 24 h, 48 h after the formation of GV biofilm respectively, and stained with Live/Dead fluorescent staining agent. The formation of GV biofilm was observed. Results: (1) under the same culture conditions, the OD values of GV biofilm formation at different stages were significantly different (P0.05). The results of scanning electron microscope showed that the isolated colony began to join at 24 h after 4 h culture, and a layer of extracellular polymer was formed at 48 h after culture. (2) the fluorescence staining of GV biofilm showed that there was no change in morphology after 48 h of culture. (2) the fluorescence staining of the biofilm showed that the colony was formed at 24 h and formed a layer of extracellular polymer after 48 h. Lactobacillus vaginalis inhibited the growth of Lactobacillus vaginalis at 0 h and 24 h after growth, the thickness of GV biofilm decreased, and formed loose colony, mainly Lactobacillus. 48 hours after growth, the colony density did not change, the area of dead bacteria increased, and the living bacteria were mainly Lactobacillus. Conclusion: (1) the formation of vaginal GV biofilm is slow, the beginning time of biofilm formation is about 24 hours after culture, and the mature biofilm is formed 48 hours later. (2) Lactobacillus vaginalis can interfere with the formation of GV biofilm. Different Lactobacillus had different effects on GV biofilm at different stages.
【作者單位】： 北京大學(xué)第一醫(yī)院婦產(chǎn)科;清華大學(xué)長庚醫(yī)院婦產(chǎn)科;
【基金】：國家自然基金面上項目(No:81170535) 國家自然青年科學(xué)基金項目(No:81200411) 教育部高等學(xué)校博士學(xué)科點專項科研基金(No:20120001120037)
【分類號】：R711.34
[Abstract]:Aim: to study the growth process of Gardnerella vaginalis (GV) biofilm in vitro and the effect of Lactobacillus vaginalis on GV biofilm. Methods: GV standard strain (ATCC14018) was selected to culture biofilm in vitro. The biofilm formation and morphology of biofilm formation were observed at different stages of biofilm formation. Lactobacillus vaginalis 1 # (rhamnose Lactobacillus) and 2 # (Lactobacillus casei) were co-cultured for 24 hours at 0 h, 24 h, 48 h after the formation of GV biofilm respectively, and stained with Live/Dead fluorescent staining agent. The formation of GV biofilm was observed. Results: (1) under the same culture conditions, the OD values of GV biofilm formation at different stages were significantly different (P0.05). The results of scanning electron microscope showed that the isolated colony began to join at 24 h after 4 h culture, and a layer of extracellular polymer was formed at 48 h after culture. (2) the fluorescence staining of GV biofilm showed that there was no change in morphology after 48 h of culture. (2) the fluorescence staining of the biofilm showed that the colony was formed at 24 h and formed a layer of extracellular polymer after 48 h. Lactobacillus vaginalis inhibited the growth of Lactobacillus vaginalis at 0 h and 24 h after growth, the thickness of GV biofilm decreased, and formed loose colony, mainly Lactobacillus. 48 hours after growth, the colony density did not change, the area of dead bacteria increased, and the living bacteria were mainly Lactobacillus. Conclusion: (1) the formation of vaginal GV biofilm is slow, the beginning time of biofilm formation is about 24 hours after culture, and the mature biofilm is formed 48 hours later. (2) Lactobacillus vaginalis can interfere with the formation of GV biofilm. Different Lactobacillus had different effects on GV biofilm at different stages.
【作者單位】： 北京大學(xué)第一醫(yī)院婦產(chǎn)科;清華大學(xué)長庚醫(yī)院婦產(chǎn)科;
【基金】：國家自然基金面上項目(No:81170535) 國家自然青年科學(xué)基金項目(No:81200411) 教育部高等學(xué)校博士學(xué)科點專項科研基金(No:20120001120037)
【分類號】：R711.34
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相關(guān)期刊論文 前1條
1 林思瑤;羅新;蔣學(xué)風(fēng);;加德納菌生物膜——細(xì)菌性陰道病新治療靶點[J];國際婦產(chǎn)科學(xué)雜志;2011年06期
【共引文獻(xiàn)】
相關(guān)期刊論文 前6條
1 余z衙，
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