【摘要】：目的探討慢速冷凍和玻璃化冷凍方法對人卵裂期冷凍胚胎移植周期妊娠結局的影響。方法回顧性分析2011年1月~2015年12月在北京大學第一醫(yī)院進行卵裂期冷凍胚胎移植的1331個周期,包括慢速冷凍周期431個和玻璃化冷凍周期900個,比較兩種不同冷凍方法的胚胎復蘇率、完整胚胎率、臨床妊娠率、種植率、流產率等各項指標,并分析卵裂球損傷對胚胎發(fā)育潛能的影響。結果玻璃化冷凍的胚胎復蘇率(92.53%)、完整胚胎率(75.43%)、臨床妊娠率(45.72%)、種植率(27.41%)高于慢速冷凍(76.93%、48.46%、39.52%、19.88%,P0.05);而流產率分別為12.20%、12.56%(P0.05);周期取消率分別為3.71%、1.33%(P0.05)。慢速冷凍移植0、1、2、3個無卵裂球損傷胚胎的臨床妊娠率分別為:36.2%、37.7%、42.0%、44.3%(P0.05);玻璃化冷凍移植0、1、2、3個無卵裂球損傷胚胎的臨床妊娠率分別為:20.5%、42.3%、48.8%、54.1%(P0.05)。結論玻璃化冷凍法更適合于人卵裂期胚胎冷凍保存,其冷凍胚胎移植周期的妊娠結局要優(yōu)于慢速冷凍法;卵裂球損傷對玻璃化冷凍復蘇胚胎的發(fā)育潛能影響較大。
[Abstract]:Objective to investigate the effects of slow freezing and vitrification on pregnancy outcome in human cleavage cryopreserved embryo transfer cycle. Methods from January 2011 to December 2015, 1331 cycles of cryopreservation embryo transfer were performed in the first Hospital of Peking University, including 431 cycles of slow freezing and 900 cycles of vitrification. The embryo resuscitation rate, intact embryo rate, clinical pregnancy rate, implantation rate and abortion rate of two different freezing methods were compared, and the effects of blastomere injury on embryo development potential were analyzed. Results the embryo resuscitation rate (92.53%), complete embryo rate (75.43%), clinical pregnancy rate (45.72%), implantation rate (27.41%) in vitrification group were higher than that in slow freezing (76.93%) (48.46%). 19.8810 (P0.05); The abortion rate was 12.20% (P0.05) and the cycle cancellation rate was 3.71% (P0.05). The clinical pregnancy rate of the embryos without blastocyst injury were 36.2% and 42.0% (P0.05) respectively. The clinical pregnancy rate of vitrified cryopreserved embryos was 20.5% (P 0.05) and 48.3% (P 0.05). Conclusion vitrified cryopreservation is more suitable for cryopreservation of human embryos at cleavage stage, the pregnancy outcome of cryopreserved embryo transfer cycle is better than that of slow freezing method, and cleavage ball injury has great influence on the development potential of vitrified cryopreservation embryos.
【作者單位】： 北京大學第一醫(yī)院婦產科;北京大學第一醫(yī)院泌尿科;
【分類號】：R714.8

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