【摘要】：目的:觀察基質(zhì)成纖維細(xì)胞活化促進(jìn)子宮肌瘤細(xì)胞的增殖作用,探討基質(zhì)成纖維細(xì)胞在子宮肌瘤發(fā)病機(jī)制中的作用。方法:采用免疫磁珠法分選子宮肌瘤組織和平滑肌組織的成纖維細(xì)胞。用免疫熒光及Western blot法檢測成纖維細(xì)胞活化標(biāo)記物(FAP)的表達(dá);觀察肌瘤基質(zhì)成纖維細(xì)胞培養(yǎng)上清對(duì)平滑肌基質(zhì)成纖維細(xì)胞(Fib)、平滑肌細(xì)胞(SMC)、肌瘤細(xì)胞(UFC)的活化作用及增殖活力的影響。采用RNAi技術(shù)沉默肌瘤基質(zhì)成纖維細(xì)胞的FAP基因,觀察細(xì)胞增殖活力及ECM、生長因子的表達(dá)變化,同時(shí)觀察細(xì)胞增殖通路相關(guān)信號(hào)分子的改變。結(jié)果:肌瘤組織成纖維細(xì)胞中FAP表達(dá)高于Fib;肌瘤基質(zhì)成纖維細(xì)胞培養(yǎng)上清能增加Fib的FAP表達(dá)強(qiáng)度,并促進(jìn)細(xì)胞增殖活力;沉默F(xiàn)AP基因,可抑制肌瘤基質(zhì)成纖維細(xì)胞ECM(collgen I、fibronectin、laminin)、生長因子(TGF-β、IGF-1)及細(xì)胞增殖通路相關(guān)信號(hào)分子(Akt、ERK、c-Fos、MEK)的表達(dá)。結(jié)論:子宮肌瘤基質(zhì)成纖維細(xì)胞的活化程度高于子宮平滑肌組織基質(zhì)成纖維細(xì)胞;活化的基質(zhì)成纖維細(xì)胞具有促細(xì)胞增殖效應(yīng),提示活化的基質(zhì)成纖維細(xì)胞在子宮肌瘤發(fā)生過程可能具有重要作用。
[Abstract]:Aim: to investigate the role of stromal fibroblasts in the pathogenesis of uterine leiomyoma. Methods: fibroblasts from uterine leiomyoma and smooth muscle tissue were separated by immunomagnetic beads. The expression of fibroblast activation marker (FAP) was detected by immunofluorescence and Western blot. To observe the effect of the supernatant of fibroblast culture on the activation and proliferation of (UFC) in (Fib), smooth muscle cell (SMC),) leiomyoma cells of smooth muscle matrix fibroblasts (SMC). RNAi technique was used to silence the FAP gene in fibroblasts of fibroblasts, to observe the changes of cell proliferation activity and ECM, growth factor expression, and to observe the changes of signal molecules associated with cell proliferation pathway. Results: the expression of FAP in fibroblasts of fibroblasts was higher than that in the culture supernatants of Fib; fibroblasts, which could increase the FAP expression of Fib and promote the proliferation of fibroblasts. Silencing FAP gene could inhibit the expression of ECM (collgen Ifibronectin laminin, TGF- 尾 (IGF-1) and Akt,ERK,c-Fos,MEK. Conclusion: the activation of stromal fibroblasts in uterine leiomyoma is higher than that of stromal fibroblasts in uterine smooth muscle tissue. The activated stromal fibroblasts have the effect of promoting cell proliferation, suggesting that activated stromal fibroblasts may play an important role in the development of uterine leiomyoma.
【作者單位】： 同濟(jì)大學(xué)附屬楊浦醫(yī)院婦產(chǎn)科;同濟(jì)大學(xué)醫(yī)學(xué)院婦科微創(chuàng)醫(yī)學(xué)研究所;
【分類號(hào)】：R737.33

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