【摘要】：[目的]研究毒蕈堿受體激動(dòng)劑乙酰膽堿(ACh, acetylcholine)對正常人臍帶血管的舒縮功能的影響及其相應(yīng)機(jī)制并與其對大鼠和羊血管舒縮功能的作用進(jìn)行比較研究。 [方法](1)臨床收集正常足月新生兒臍帶；(2)收集孕21天胎鼠臍帶,分別分離活性離體動(dòng)靜脈臍帶血管；(3)收集正常成年雄性大鼠胸主動(dòng)脈、腸系膜動(dòng)脈,孕晚期胎羊頸動(dòng)脈,腸系膜動(dòng)脈和股動(dòng)脈。分別將分離后的血管剪成3-5mm長度的血管環(huán),置于5ml Krebs37℃恒溫水浴槽內(nèi),進(jìn)行大血管和微血管功能實(shí)驗(yàn)測定ACh對血管張力的影響。同時(shí),本實(shí)驗(yàn)采用RT-PCR技術(shù)檢測了人臍血管中M受體亞型的分布。 【結(jié)果】ACh、卡巴膽堿cabarchol (10-11-10-3mol/L)對臍帶血管表現(xiàn)為較強(qiáng)的劑量依賴性收縮作用,而且在低濃度時(shí)也并未觀察到其對臍帶血管的舒張作用。ACh、cabarchol對臍帶血管的收縮作用可被阿托品(10-6mo1/L)完全阻斷,在基礎(chǔ)血管張力下,尼古丁(nicotine,10-11-10"3mol/L)對臍帶血管張力沒有明顯影響,但通過抑制血管內(nèi)皮NO合成可使臍帶血管對ACh的收縮反應(yīng)增強(qiáng),而PKC通路阻斷劑和L-Ca通道阻斷劑則可降低ACh的收縮反應(yīng)。RT-PCR結(jié)果顯示,除M1受體在臍帶血管中表達(dá)量較少以外,M2-M5的rn-RNA表達(dá)水平基本相似。 【結(jié)論】ACh對正常人臍帶血管表現(xiàn)為較強(qiáng)的收縮作用而不是舒張作用,實(shí)驗(yàn)表明ACh對臍帶血管的收縮作用與M受體下游的L-Ca、PKC通路有關(guān)。
[Abstract]:[objective] to study the effects of muscarinic receptor agonist acetylcholine (ACh, acetylcholine) on the systolic and diastolic function of normal umbilical cord blood vessels in rats and sheep. [methods] (1) normal umbilical cord of full-term newborns was collected clinically, (2) umbilical cord of 21 day pregnant rats was collected, and active isolated umbilical cord blood vessels were isolated. (3) the thoracic aorta, mesenteric artery, fetal goat carotid artery, mesenteric artery and femoral artery were collected from normal adult male rats. The isolated blood vessels were cut into 3-5mm long vascular rings and placed in the 5ml Krebs37 鈩，

本文編號：2409465