【摘要】：目的觀察過表達(dá)微小RNA124(miR-124)對宮頸癌Siha細(xì)胞放射敏感性的影響,并探討其機(jī)制。方法將宮頸癌Siha細(xì)胞分為觀察組和對照組,觀察組轉(zhuǎn)染miR-124模擬物,對照組不轉(zhuǎn)染。采用實時熒光定量PCR法檢測兩組細(xì)胞miR-124和信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子3(STAT3)mRNA表達(dá)量。采用克隆形成實驗檢測兩組細(xì)胞放射敏感性。采用流式細(xì)胞術(shù)檢測兩組細(xì)胞周期分布和放射后細(xì)胞凋亡率。結(jié)果觀察組、對照組D0分別為1.062、1.542Gy,Dq分別為1.605、1.970 Gy,SF2分別為0.527、0.685。觀察組相對于對照組的放射增敏比為1.451。轉(zhuǎn)染后24h觀察組、對照組miR-124相對表達(dá)量分別為89.3±13.6、1.0±0.1,STAT3 mRNA相對表達(dá)量分別為0.3±0.03、1.0±0.1,兩組相比,P均0.05。觀察組G0/G1期細(xì)胞比例為82.49%±1.97%、S期細(xì)胞比例11.87%±1.38%、G2/M期細(xì)胞比例為5.64%±0.72%;對照組分別為74.58%±1.28%、19.88%±0.26%、5.54%±1.05%。觀察組G0/G1期比例高于對照組組,S期比例低于對照組(P均0.05),兩組G2/M期細(xì)胞比例相比P0.05。觀察組、對照組細(xì)胞X線照射后細(xì)胞凋亡率分別為45.87%±3.16%、37.27%±0.87%,兩組相比,P0.05。結(jié)論過表達(dá)miR-124可能提高宮頸癌Siha細(xì)胞的放射敏感性。其機(jī)制可能是過表達(dá)miR-124會抑制宮頸癌Siha細(xì)胞STAT3表達(dá),進(jìn)而阻滯細(xì)胞周期于G0/G1期,促進(jìn)細(xì)胞凋亡,從而提高宮頸癌Siha細(xì)胞的放射敏感性。
[Abstract]:Objective to investigate the radiosensitivity of cervical cancer Siha cells induced by overexpression of small RNA124 (miR-124) and its mechanism. Methods Cervical carcinoma Siha cells were divided into observation group and control group. The miR-124 mimics were transfected in the observation group, but not in the control group. The expression of miR-124 and signal transduction and transcription activator 3 (STAT3) mRNA was detected by real-time fluorescence quantitative PCR. The radiosensitivity of two groups of cells was detected by clone forming assay. Cell cycle distribution and apoptosis rate after radiation were detected by flow cytometry. Results in the observation group, the D0 of the control group was 1.062 (1.542Gy) and the DQ was 1.6051.970 Gy,SF2 (0.527) 0.685respectively. The radiosensitization ratio of the observation group was 1.451compared with that of the control group. 24 hours after transfection, the relative expression of miR-124 in the control group was 89.3 鹵13.6N 1.0 鹵0.1 鹵0.1, respectively. The relative expression of STAT3 mRNA in the control group was 0.3 鹵0.03 鹵1.0 鹵0.1, respectively, compared with that in the two groups (P < 0.05). In the observation group, the percentage of cells in G0/G1 phase was 82.49% 鹵1.97% and the proportion of cells in S phase was 11.87% 鹵1.38% in G _ 2 / M phase, 5.64% 鹵0.72% in G _ 2 / M phase. The control group was 74.58% 鹵1.28% 鹵19.88% 鹵0.26% and 5.54% 鹵1.05% respectively. The ratio of G0/G1 phase and S phase in the observation group was higher than that in the control group (P 0.05), and the ratio of G 2 / M phase cells in the two groups was lower than that in the control group (P 0.05). In the observation group, the apoptosis rate of the cells in the control group was 45.87% 鹵3.16% and 37.27% 鹵0.87%, respectively, compared with that in the control group (P 0.05). Conclusion overexpression of miR-124 may enhance the radiosensitivity of cervical cancer Siha cells. The mechanism may be that overexpression of miR-124 can inhibit the expression of STAT3 in cervical cancer Siha cells, thus block cell cycle in G0/G1 phase, promote cell apoptosis, and thus enhance the radiosensitivity of cervical cancer Siha cells.
【作者單位】： 蚌埠醫(yī)學(xué)院研究生院;泰興市人民醫(yī)院;
【基金】：蚌埠醫(yī)學(xué)院研究生科研創(chuàng)新計劃(Byycx1624)
【分類號】：R737.33

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