【摘要】：目的：卵巢癌是最常見(jiàn)的婦科惡性腫瘤，其發(fā)病率和死亡率居?jì)D科惡性腫瘤之首，5年生存率僅為30%~40%。過(guò)繼細(xì)胞免疫治療越來(lái)越成為卵巢癌治療的重要武器，本研究通過(guò)觀察DC-CIK(dendriticcell-cytokineinducedkillercell)過(guò)繼細(xì)胞免疫治療對(duì)卵巢癌患者外周血淋巴細(xì)胞亞群及相關(guān)細(xì)胞因子的變化，評(píng)價(jià)DC-CIK細(xì)胞免疫治療對(duì)卵巢癌臨床療效。 方法： 選取2010年3月至2013年3月在吉林大學(xué)第二醫(yī)院腫瘤生物治療中心治療的22例卵巢癌患者，有明確的組織病理學(xué)檢查。取外周血分離單個(gè)核細(xì)胞(PBMC)，在體外誘導(dǎo)出DC和CIK細(xì)胞，分別行7個(gè)周期的DC-CIK細(xì)胞免疫治療，采用流式細(xì)胞術(shù)和酶聯(lián)免疫法檢測(cè)接受DC-CIK免疫治療不同周期后，，其外周血中淋巴細(xì)胞亞群（包括T淋巴細(xì)胞亞群（CD3+）、輔助性T細(xì)胞亞群（CD3+/CD4+）、殺傷性T細(xì)胞亞群（CD3+/CD8+）、NKT細(xì)胞亞群（CD3+/CD16+56+）等）的變化，及相關(guān)因子白介素-2、腫瘤壞死因子-α、干擾素-γ的表達(dá)水平。同時(shí)對(duì)變態(tài)反應(yīng)、血液系統(tǒng)、心血管系統(tǒng)、肝臟、腎臟、胃腸道、全身癥狀等進(jìn)行監(jiān)測(cè)，并進(jìn)行安全性評(píng)價(jià)。利用SPSS13.0軟件包進(jìn)行數(shù)據(jù)的分析和處理。 結(jié)果： 1.與治療前相比，4周期及7個(gè)周期DC-CIK細(xì)胞免疫治療組患者外周血T淋巴細(xì)胞亞群無(wú)顯著變化，即T淋巴細(xì)胞亞群（CD3+）、輔助性T細(xì)胞亞群（CD3+/CD4+）、殺傷性T細(xì)胞亞群（CD3+/CD8+）、NKT細(xì)胞亞群（CD3+/CD16+56+）等與治療前相比略有升高，但無(wú)顯著統(tǒng)計(jì)學(xué)差異（P0.05）。 2.4周期治療后，患者外周血白介素-2、腫瘤壞死因子-α、干擾素-γ水平較治療前提高，但差異并不顯著（P0.05），而當(dāng)7個(gè)周期治療后，IL-2、TNF-α、IFN-γ水平較治療前明顯提高且具有統(tǒng)計(jì)學(xué)差異（P0.05）； 3.DC-CIK過(guò)繼性細(xì)胞免疫治療后，患者軀體功能、情緒方面較治療前改善明顯。 4.患者顯示出良好的耐受性，共計(jì)154周期的治療中，不良反應(yīng)發(fā)生率較低，發(fā)熱，蕁麻疹，心慌，肝臟損害均為一過(guò)性。 結(jié)論： 1.DC-CIK細(xì)胞免疫治療可以明顯提高卵巢癌患者免疫狀態(tài)，提高機(jī)體抗腫瘤能力，機(jī)體免疫力提升，生活質(zhì)量顯著提高。 2.臨床應(yīng)用無(wú)明顯不良反應(yīng),是卵巢癌患者重要的輔助治療手段。
[Abstract]:Objective: ovarian cancer is the most common gynecologic malignant tumor, its morbidity and mortality are the first among gynecological malignancies, and the 5-year survival rate is only 30%. Adoptive cellular immunotherapy has become an important weapon in ovarian cancer treatment. The changes of lymphocyte subsets and related cytokines in peripheral blood of patients with ovarian cancer were observed by DC-CIK (dendriticcell-cytokineinducedkillercell) adoptive cellular immunotherapy. To evaluate the clinical effect of DC-CIK cell immunotherapy on ovarian cancer. Methods: from March 2010 to March 2013, 22 patients with ovarian cancer treated in tumor biotherapy center of the second Hospital of Jilin University were examined by histopathological examination. DC and CIK cells were induced from peripheral blood mononuclear cells (PBMC),) in vitro and were treated with 7 cycles of DC-CIK cell immunotherapy respectively. Flow cytometry and enzyme-linked immunosorbent assay (Elisa) were used to detect the different cycles of DC-CIK immunotherapy. The changes of lymphocyte subsets (including T lymphocyte subsets (CD3), helper T cell subsets (CD3 / CD4), CD3 / CD8), NKT cell subsets (CD3 / CD16 56) in peripheral blood. The expression level of Interleukin-2, tumor necrosis factor-偽 and interferon-緯. Allergy, blood system, cardiovascular system, liver, kidney, gastrointestinal tract, systemic symptoms were monitored and safety was evaluated. SPSS13.0 software package is used to analyze and process the data. Results: 1. There were no significant changes in peripheral blood T lymphocyte subsets (CD3), helper T cell subsets (CD3 / CD4) in patients with 4 cycles and 7 cycles of DC-CIK cell immunotherapy compared with those before treatment. The lethal T cell subsets (CD3 / CD8), NKT cell subsets) (CD3 / CD16 56) were slightly higher than those before treatment, but there was no significant difference (P0.05). The levels of interleukin-2, tumor necrosis factor- 偽 and interferon- 緯 in peripheral blood were increased after 2. 4 cycles treatment, but the difference was not significant (P0.05). However, after 7 cycles of treatment, the levels of IL-2,TNF- 偽, TNF- 偽 and IFN- 緯 in peripheral blood of the patients were significantly higher than those before treatment (P0.05). The level of IFN- 緯 was significantly higher than that before treatment (P0.05). After adoptive cellular immunotherapy with 3.DC-CIK, the somatic function and mood of the patients improved significantly. 4. In 154 cycles of treatment, the incidence of adverse reactions was low, fever, urticaria, palpitation, and liver damage were transient. Conclusion: 1.DC-CIK cell immunotherapy can significantly improve the immune status of patients with ovarian cancer, improve the ability of anti-tumor, enhance the immunity of the body, and improve the quality of life. 2. There is no obvious adverse reaction in clinical application and it is an important adjuvant therapy for ovarian cancer patients.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.31

【參考文獻(xiàn)】

相關(guān)期刊論文 前9條

1 陳斯?jié)?陳雪梅;丁穎;王希成;張帆;莫?jiǎng)P嵐;;DC方案與PF方案化療與同步放療治療中晚期食管鱗癌[J];南方醫(yī)科大學(xué)學(xué)報(bào);2011年04期

2 應(yīng)敏剛;鄭秋紅;陳奕貴;謝云青;龔福生;陳路川;吳君心;鄭天榮;;CIK與DC細(xì)胞聯(lián)合治療145例晚期惡性實(shí)體瘤[J];福建醫(yī)科大學(xué)學(xué)報(bào);2007年03期

3 葉韻斌;彭峰;李潔羽;林麗平;鄭雄偉;周東;力超;黎巧連;蔡曉雯;;肝細(xì)胞肝癌組織浸潤(rùn)性淋巴細(xì)胞及其因子的表達(dá)[J];細(xì)胞與分子免疫學(xué)雜志;2011年10期

4 魏緒倉(cāng);翟欣輝;韓秀蕊;楊娣娣;趙文理;;DC-CIK細(xì)胞的生物學(xué)活性及體外抗白血病作用的研究(英文)[J];中國(guó)實(shí)驗(yàn)血液學(xué)雜志;2008年05期

5 朱建平;朱壽興;朱美萍;邵力正;徐鳴;;CIK細(xì)胞輔助治療卵巢癌的臨床療效[J];江蘇醫(yī)藥;2008年04期

6 李芳,朱懷仕,賈平,高慶蕾,徐茜,劉德艷,王世宣,盧運(yùn)萍,馬丁;卵巢癌患者自體腫瘤細(xì)胞疫苗的臨床應(yīng)用[J];中華婦產(chǎn)科雜志;2004年01期

7 李慧;任秀寶;張澎;安秀梅;劉虹;郝希山;;樹(shù)突狀細(xì)胞對(duì)CIK細(xì)胞中CD_4~+CD_(25)~+T細(xì)胞數(shù)量及免疫調(diào)節(jié)作用的影響[J];中華醫(yī)學(xué)雜志;2005年44期

8 楊葳;徐銘寶;;CIK細(xì)胞治療惡性腫瘤研究進(jìn)展[J];中國(guó)實(shí)用醫(yī)藥;2009年29期

9 李敏;鄧海峰;陸明洋;徐斌;鄭曉;劉檢;吳駿;季枚;周怡;孫青;吳昌平;蔣敬庭;;CIK治療前后腫瘤患者外周血CD4~+ CD25~+調(diào)節(jié)型T細(xì)胞變化研究[J];中國(guó)實(shí)驗(yàn)診斷學(xué);2011年06期

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