【摘要】：目的：研究Wnt-1蛋白與Sfrp-1蛋白在宮腔粘連患者子宮內(nèi)膜中的表達(dá)情況,初步探討其與宮腔粘連的相關(guān)性。 方法：采用免疫組織化學(xué)(IHC)方法,檢測(cè)60例宮腔粘連(Intrauterin adhesions,IUA)患者子宮內(nèi)膜組織(輕、中、重度IUA各20例)及20例子宮縱隔患者正常子宮內(nèi)膜組織中的Wnt-1蛋白與Sfrp-1蛋白的表達(dá)差異,分析宮腔粘連患者子宮內(nèi)膜中Wnt-1蛋白和Sfrp-1蛋白的表達(dá)與宮腔粘連程度的相關(guān)性。 結(jié)果：1.Wnt-1蛋白在子宮內(nèi)膜腺上皮細(xì)胞及間質(zhì)細(xì)胞中的胞膜和胞漿表達(dá),Wnt-1蛋白在IUA組中表達(dá)高于非IUA組(P0.05),且Wnt-1蛋白在重度IUA組表達(dá)高于中度IUA組,中度IUA組高于輕度IUA組(P0.05).2.Sfrp-1蛋白主要在子宮內(nèi)膜腺上皮細(xì)胞及間質(zhì)細(xì)胞的胞漿中表達(dá),部分在胞膜表達(dá),Sfrp-1蛋白在非IUA組中表達(dá)高于IUA組(P0.05),且Sfrp-1蛋白在輕度IUA組高于中度IUA組,中度IUA組高于重度IUA組(P0.05)。3.Wnt-1蛋白和Sfrp-1蛋白在IUA患者子宮內(nèi)膜中的表達(dá)呈負(fù)相關(guān)(相關(guān)系數(shù)r=-0.713)。 結(jié)論：1.Wnt-1可能與宮腔粘連的形成及嚴(yán)重程度有關(guān)。2.Sfrp-1可能在宮腔粘連的形成中起抑制作用。3.Wnt-1和Sfrp-1可能相互作用參與宮腔粘連的形成。 圖片9幅,表7個(gè),文獻(xiàn)58篇
[Abstract]:Aim: to investigate the expression of Wnt-1 protein and Sfrp-1 protein in endometrium of patients with intrauterine adhesions. Methods: the endometrial tissues of 60 patients with intrauterine adhesion (Intrauterin adhesions,IUA) were detected by immunohistochemical (IHC) method. The expression of Wnt-1 protein and Sfrp-1 protein in normal endometrium of 20 cases of mediastinum and 20 cases of severe IUA were different. To analyze the correlation between the expression of Wnt-1 protein and Sfrp-1 protein and the degree of intrauterine adhesion in endometrium of patients with intrauterine adhesion. Results: the expression of 1.Wnt-1 protein in the membrane and cytoplasm of endometrial glandular epithelial cells and stromal cells was higher in IUA group than in non-IUA group (P0.05), and the expression of Wnt-1 protein in severe IUA group was higher than that in moderate IUA group. The expression of 2.Sfrp-1 protein was mainly in the cytoplasm of endometrial glandular epithelial cells and interstitial cells in moderate IUA group than in mild IUA group (P0.05). The expression of Sfrp-1 protein in non-IUA group was higher than that in IUA group (P0.05). The expression of Sfrp-1 protein in the mild IUA group was higher than that in the moderate IUA group, and in the moderate IUA group was higher than that in the severe IUA group (P0.05). The expression of 3.Wnt-1 protein and Sfrp-1 protein in the endometrium of IUA patients was negatively correlated (r = -0.713). Conclusion: 1.Wnt-1 may be related to the formation and severity of intrauterine adhesions. 2.Sfrp-1 may play an inhibitory role in the formation of intrauterine adhesions. 3.Wnt-1 and Sfrp-1 may interact in the formation of intrauterine adhesions. 9 pictures, 7 tables, 58 documents
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R711.74

【參考文獻(xiàn)】

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1 鄭穎;張t，

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