【摘要】：目的探討順鉑對(duì)人子宮內(nèi)膜癌RL-952細(xì)胞系Matriptase,uPA-mRNA表達(dá)的影響。方法體外培養(yǎng)人子宮內(nèi)膜癌RL-952細(xì)胞,采用反轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)檢測(cè)順鉑對(duì)人子宮內(nèi)膜癌RL-952細(xì)胞Matriptase,uPA-mRNA表達(dá)的影響。結(jié)果 Matriptase,uPA-mRNA在人子宮內(nèi)膜癌RL-952細(xì)胞系呈陽性表達(dá)。順鉑可下調(diào)Matriptase,uPA-mRNA在人子宮內(nèi)膜癌RL-952細(xì)胞系的表達(dá),具有濃度依賴性。8mg/L順鉑溶液作用不同時(shí)間后,4h后RL-952細(xì)胞Matriptase-mRNA表達(dá)量即明顯下降(P0.05),而uPA-mRNA表達(dá)量于作用8h后才開始下降(P0.05),具有時(shí)間依賴性。結(jié)論順鉑可抑制人子宮內(nèi)膜癌RL-952細(xì)胞系Matriptase,uPA-mRNA的表達(dá),并具有濃度依賴性。8 mg/L的順鉑作用于人子宮內(nèi)膜癌RL-952細(xì)胞后,Matriptase-mRNA比uPA-mRNA更早出現(xiàn)表達(dá)下調(diào)的變化。
[Abstract]:Objective to investigate the effect of cisplatin on the expression of Matriptase,uPA-mRNA in human endometrial carcinoma RL-952 cell line. Methods Human endometrial carcinoma RL-952 cells were cultured in vitro. The effect of cisplatin on the expression of Matriptase,uPA-mRNA in human endometrial carcinoma RL-952 cells was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results the expression of Matriptase,uPA-mRNA was positive in human endometrial carcinoma RL-952 cell line. Cisplatin could down-regulate the expression of Matriptase,uPA-mRNA in human endometrial carcinoma RL-952 cell line in a concentration-dependent manner. The expression of Matriptase-mRNA in RL-952 cells decreased significantly 4 hours after treatment with 8mg/L cisplatin solution (P0.05). However, the expression of uPA-mRNA began to decrease after 8 h treatment (P0.05), which was time-dependent. Conclusion Cisplatin can inhibit the expression of Matriptase,uPA-mRNA in human endometrial carcinoma RL-952 cell line in a concentration-dependent manner. 8 mg/L cisplatin can inhibit the expression of Matriptase,uPA-mRNA in human endometrial carcinoma RL-952 cells. The down-regulation of Matriptase-mRNA was earlier than that of uPA-mRNA.
【作者單位】： 福建醫(yī)科大學(xué)附屬第二醫(yī)院婦產(chǎn)科;福建省婦幼保健院婦科腫瘤實(shí)驗(yàn)室;廈門市中醫(yī)院婦產(chǎn)科;福建醫(yī)科大學(xué)附屬協(xié)和臨床醫(yī)學(xué)院;
【基金】：福建省自然科學(xué)基金(2012J01310)
【分類號(hào)】：R737.33

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