【摘要】：目的： 分析子宮內膜腺癌組織中CD68和YKL-40蛋白的表達水平，探討二者與子宮內膜癌臨床病理特征之間的關系及其臨床意義。方法： 1.采用免疫組織化學Elivision方法檢測CD68和YKL-40蛋白分別在20例正常子宮內膜（normal endometrium，NE）、20例子宮內膜不典型增生（endometrialatypical hyperplasia，EAH）和56例子宮內膜腺癌（endometrial adenocarcinoma，EAC）中的表達。2.運用SPSS17.0統(tǒng)計軟件對所有研究的數據進行統(tǒng)計學分析和處理。 結果： 1.在NE、EAH、EAC三組組織中均可見CD68蛋白的陽性表達。CD68在NE、EAH、EAC組織中的陽性表達率分別為30%、55%、80.4%。EAC組織中CD68蛋白的陽性表達率明顯高于EAH組織（X2=4.887，P=0.0270.05），且也顯著高于NE組織（X2=16.929，P=0.0000.05）；EAH組織中CD68蛋白的陽性表達率與NE組織差異無統(tǒng)計學意義（X2=2.558，P=0.110.05）。在EAC組織中CD68蛋白的表達與腫瘤的組織學分級、肌層浸潤、及淋巴結轉移有關(P=0.019，P=0.047，P=0.005，P均0.05)，而與患者年齡及臨床分期無關(P=1.0，P=0.697，P均0.05)。 2.YKL-40蛋白在NE、EAH、EAC三組組織中均可見陽性表達。YKL-40蛋白在NE、EAH、EAC組織中的陽性表達率分別為25%、40%、76.8%。EAC組織中YKL-40蛋白的陽性表達率明顯高于EAH組織（X2=9.034， P=0.0030.05），且也同樣顯著高于NE組織（X2=16.984，P=0.0000.05）；EAH組織中YKL-40蛋白的陽性表達率與NE組織差異無統(tǒng)計學意義（X2=1.026，P=0.3110.05）。在EAC組織中YKL-40蛋白的表達與腫瘤的組織學分級有關(P=0.008，P0.05)，而與患者年齡、臨床分期、肌層浸潤和淋巴結轉移無關(P=0.186，P=0.852，，P=0.511，P=0.752，P均0.05)。 3.經Spearman相關分析顯示：CD68與YKL-40蛋白在EAC組織中的表達有相關性，且呈正相關（rs=0.367，P=0.005）。 結論： 1. CD68和YKL-40蛋白在NE、EAH、EAC三組組織中的陽性表達率呈逐步升高的趨勢。 2.隨著EAC患者的組織分化程度降低、肌層浸潤加深和淋巴結轉移，CD68蛋白的陽性表達率更高。EAC患者中YKL-40蛋白隨組織分化程度降低而陽性表率也更高。 3. CD68和YKL-40蛋白在EAC組織中過表達且呈正相關。
[Abstract]:Objective: to analyze the expression of CD68 and YKL-40 protein in endometrial adenocarcinoma and to explore the relationship between them and the clinicopathological features of endometrial carcinoma. Methods: 1. Immunohistochemical Elivision was used to detect the expression of CD68 and YKL-40 protein in 20 cases of normal endometrium (normal endometrium,NE), 20 cases of endometrial atypical hyperplasia (endometrialatypical hyperplasia,EAH) and 56 cases of endometrial adenocarcinoma (endometrial adenocarcinoma,EAC). The data of all the studies were analyzed and processed by SPSS17.0 software. Results: 1. The positive expression of CD68 protein was found in all three groups of NE,EAH,EAC, and the positive expression rate of CD68 in NE,EAH,EAC tissue was 30% and 55%, respectively. The positive expression rate of CD68 protein in 80.4%.EAC tissues was significantly higher than that in EAH tissues (X2O4.887Pu 0.0270.05), and also higher than that in NE tissues (X2O16.929PU 0.0000.05). The positive expression rate of CD68 protein in EAH was not significantly different from that in NE (X2 + 2.558 P < 0. 110.05). The expression of CD68 protein in EAC was related to histological grade, myometrial infiltration and lymph node metastasis (P0.019, P0.047, P0. 005, P 0. 05), but not to the patient's age and clinical stage (P0. 0. 0 P0. 697, P 0. 697, P 0. 019, P 0. 047, P 0. 005, P 0. 05). P 0.05). Positive expression of 2.YKL-40 protein was found in all three groups of NE,EAH,EAC. The positive expression rates of YKL-40 protein in NE,EAH,EAC tissues were 25% and 40%, respectively. The positive expression rate of YKL-40 protein in 76.8%.EAC was significantly higher than that in EAH (X2 + 9.034, P0. 0030. 05), and was also significantly higher than that in NE (X2 + 16. 984, P0. 0000. 05). The positive expression rate of YKL-40 protein in EAH was not significantly different from that in NE (X _ 2 ~ (2 +) ~ (1.026) P ~ (0.3110.05). The expression of YKL-40 protein in EAC was correlated with the histological grade of the tumor (P0. 008P 0.05), but not with age, clinical stage, myometrial invasion and lymph node metastasis (P0. 186 P0. 852P0. 511 P0. 752, P = 0. 752). P 0.05). 3. Spearman correlation analysis showed that there was a positive correlation (rs=0.367,P=0.005) between CD68 and YKL-40 protein expression in EAC tissues. Conclusion: 1. The positive expression rate of CD68 and YKL-40 protein in three groups of NE,EAH,EAC increased gradually. 2. With the decrease of tissue differentiation, myometrial infiltration and lymph node metastasis, the positive expression rate of CD68 protein was higher in EAC patients, and the positive rate of YKL-40 protein was higher with the decrease of tissue differentiation degree in EAC patients. 3. The overexpression of CD68 and YKL-40 protein in EAC tissues was positively correlated.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.33

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