發(fā)布時(shí)間：2018-10-19 07:21

【摘要】：目的:觀察楊梅素對人卵巢癌SKOV3細(xì)胞的生長抑制及凋亡誘導(dǎo)作用,并探討其可能機(jī)制。方法:體外培養(yǎng)人卵巢癌SKOV3細(xì)胞,按楊梅素給藥濃度將細(xì)胞分為對照組和低、中、高濃度楊梅素組,楊梅素給藥濃度分別為0、20、40和60mg·L-1。MTT法檢測各組SKOV3細(xì)胞存活率;激光共聚焦顯微鏡觀察各組SKOV3細(xì)胞核形態(tài)變化并計(jì)算異常細(xì)胞核比率;免疫熒光法檢測各組SKOV3細(xì)胞中凋亡相關(guān)蛋白-活化半胱氨酸蛋白酶3(active Caspase-3)的表達(dá);Western blotting法檢測各組SKOV3細(xì)胞中DNA損傷相關(guān)蛋白γ-H2AX的表達(dá)水平。結(jié)果:與對照組比較,低、中和高濃度楊梅素組SKOV3細(xì)胞存活率明顯下降(t=-12.35,t=-11.42,t=-10.21,P0.05);激光共聚焦顯微鏡下各濃度楊梅素組細(xì)胞出現(xiàn)核皺縮等凋亡形態(tài)學(xué)改變,且細(xì)胞中異常細(xì)胞核比率高于對照組(t=6.87,t=9.35,t=7.22,P0.05),高濃度楊梅素組異常細(xì)胞核比率高于低、中濃度楊梅素組(t=9.55,t=7.25,P0.05);隨著楊梅素給藥濃度的增加,SKOV3細(xì)胞中active Caspase-3的表達(dá)水平有升高趨勢,各濃度楊梅素組SKOV3細(xì)胞中γ-H2AX的表達(dá)水平高于對照組(t=5.41,t=3.21,t=6.58,P0.05)。結(jié)論:楊梅素可抑制人卵巢癌SKOV3細(xì)胞增殖并誘導(dǎo)其凋亡,DNA雙鏈斷裂可能是其促凋亡的機(jī)制之一。
[Abstract]:Aim: to investigate the effect of myricetin on the growth inhibition and apoptosis induction of human ovarian cancer SKOV3 cells and its possible mechanism. Methods: human ovarian cancer SKOV3 cells were cultured in vitro. The cells were divided into two groups according to the concentration of myricetin: control group and low concentration group. The survival rate of SKOV3 cells was detected by 60mg L-1.MTT method. The morphologic changes of SKOV3 nuclei were observed by laser confocal microscope and the abnormal nuclear ratio was calculated. The expression of apoptosis-related protein-activated cysteine protease 3 (active Caspase-3) in SKOV3 cells was detected by immunofluorescence assay. The expression of DNA damage associated protein 緯 -H2AX in SKOV3 cells was detected by; Western blotting method. Results: compared with the control group, the survival rate of SKOV3 cells in the myricetin group was significantly lower than that in the control group (P 0.05), and the apoptotic morphologic changes such as nuclear shrinkage were observed in the myricetin group with laser confocal microscope, and the survival rate of the cells in the myricetin group was significantly lower than that in the control group (P 0.05). The percentage of abnormal nuclei in the cells was higher than that in the control group (t = 6.87), and the ratio of abnormal nuclei in the high concentration group was higher than that in the control group (P 0.05). The expression of active Caspase-3 in SKOV3 cells showed a tendency to increase with the increase of the administration concentration of myricetin, and the ratio of abnormal nuclei in the medium concentration group was higher than that in the control group. The expression level of 緯-H2AX in SKOV3 cells of myricetin group was higher than that in control group. Conclusion: myricetin can inhibit the proliferation and induce apoptosis of human ovarian cancer SKOV3 cells, and DNA double strand break may be one of its mechanisms.
【作者單位】： 吉林醫(yī)藥學(xué)院基礎(chǔ)醫(yī)學(xué)院實(shí)驗(yàn)中心;吉林醫(yī)藥學(xué)院檢驗(yàn)學(xué)院;吉林大學(xué)基礎(chǔ)醫(yī)學(xué)院病理生理學(xué)系;吉林省吉林中西醫(yī)結(jié)合醫(yī)院體檢中心;吉林醫(yī)藥學(xué)院臨床醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金面上項(xiàng)目資助課題(81372793) 吉林省教育廳十二五科技項(xiàng)目資助課題(2013361) 吉林省大學(xué)生創(chuàng)新創(chuàng)業(yè)項(xiàng)目資助課題(20141027)
【分類號】：R737.31

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