【摘要】：目的探討孕中期熱暴露對大鼠胎盤和子代宮內(nèi)生長發(fā)育的影響。方法 24只健康清潔級SD大鼠受孕后隨機(jī)分為對照組和熱暴露組,每組12只。對照組孕鼠孕期全程在23±1℃的環(huán)境中飼養(yǎng),熱暴露組大鼠受孕后先在23±1℃的環(huán)境中飼養(yǎng),妊娠第8~13天飼養(yǎng)溫度調(diào)高至35±1℃,妊娠第14天回至23±1℃的環(huán)境中飼養(yǎng)。測量孕鼠不同妊娠時段的的體質(zhì)量變化,在孕末期行剖腹取胎,檢測仔鼠的數(shù)量、體質(zhì)量、身長、尾長、外觀、活/死胎數(shù)、胎盤質(zhì)量及胎盤組織HSP70、Bax、Bcl-2等蛋白表達(dá)。結(jié)果熱暴露組孕鼠孕末期體質(zhì)量及孕期體質(zhì)量增加值均小于對照組,且差異均有統(tǒng)計(jì)學(xué)意義(均P0.05);熱暴露組仔鼠的體質(zhì)量、身長、尾長均小于對照組,且差異均有統(tǒng)計(jì)學(xué)意義(均P0.05);兩組仔鼠胎盤質(zhì)量的比較差異無統(tǒng)計(jì)學(xué)意義;熱暴露組胎盤HSP70表達(dá)(HSP70/actin)和Bax/Bcl-2均高于對照組,且差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論孕中期熱暴露會影響大鼠胎盤及子代宮內(nèi)的生長發(fā)育,會誘發(fā)胎盤組織的熱休克反應(yīng)及加速胎盤組織細(xì)胞的凋亡。
[Abstract]:Objective to investigate the effect of heat exposure in the second trimester of pregnancy on the growth and development of rat placenta and offspring. Methods 24 healthy clean grade SD rats were randomly divided into control group and heat exposure group, 12 rats in each group. The pregnant rats in the control group were fed at 23 鹵1 鈩，

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