【摘要】：目的:探討Notch信號通路配體Jagged1、Dll4在子癇前期胎盤組織中的表達及意義。方法:選取輕度子癇前期患者30例(輕度組)、重度子癇前期患者30例(重度組)、正常妊娠孕婦30例(對照組),采用免疫組化法檢測其胎盤組織中Jagged1、Dll4的定位表達,采用熒光定量PCR技術(shù)和Western blot法分別檢測胎盤組織中Jagged1、Dll4 mRNA及蛋白的表達。結(jié)果:Jagged1主要表達于血管內(nèi)皮細胞和絨毛滋養(yǎng)層細胞,Dll4主要表達于血管內(nèi)皮細胞。輕、重度組胎盤組織中Jagged1、Dll4 mRNA及蛋白的表達水平均低于對照組(P0.05),且重度組低于輕度組(P0.05);對照組胎盤組織中Jagged1與Dll4在mRNA和蛋白水平上的表達均呈正相關(guān)(r=0.806和0.808,P0.05),輕度組中兩者表達的相關(guān)性減弱(r=0.675和0.560,P0.05),而在重度組中未發(fā)現(xiàn)兩者的相關(guān)性。結(jié)論:Jagged1、Dll4在子癇前期胎盤組織中表達失衡,與子癇前期的發(fā)病關(guān)系密切。
[Abstract]:Aim: to investigate the expression and significance of Notch signal pathway ligand Jagged1,Dll4 in placenta of preeclampsia. Methods: 30 cases of mild preeclampsia (mild group), 30 cases of severe preeclampsia (severe group) and 30 cases of normal pregnant women (control group) were selected. The expression of Jagged1,Dll4 in placenta was detected by immunohistochemical method. The expression of Jagged1,Dll4 mRNA and protein in placenta was detected by fluorescence quantitative PCR and Western blot. Results Jagged1 was mainly expressed in vascular endothelial cells and chorionic trophoblast cells, and Dll4 was mainly expressed in vascular endothelial cells. Light, The expression of Jagged1,Dll4 mRNA and protein in placenta of severe group was lower than that of control group (P0.05), and that of severe group was lower than that of mild group (P0.05), and the expression of Jagged1 and Dll4 in placenta tissue of control group were positively correlated with mRNA and protein levels (r = 0.806 and 0.808%, P 0.05), respectively, and the expression of Jagged1 and Dll4 in placenta of severe group was significantly lower than that of mild group (P 0.05). The correlation between the two groups was decreased (r 0.675 and 0.560 P 0.05), but no correlation was found in the severe group. Conclusion the expression of Dll4 in preeclampsia placenta is out of balance, which is closely related to the pathogenesis of preeclampsia.
【作者單位】： 鄭州大學第一附屬醫(yī)院產(chǎn)科;
【分類號】：R714.244

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